CAZyme Information: MGYG000001738_00312

Basic Information

• Species: Duncaniella sp001701225
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; Duncaniella; Duncaniella sp001701225
• CAZyme ID: MGYG000001738_00312
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
877	MGYG000001738_2|CGC4	99658.11	5.5713

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001738	3129928	MAG	Sweden	Europe

• Gene Location: Start: 125130; End: 127763 Strand: +

Full Sequence      

MKKIPAITAFAMLALGSLWVEAAVSSRTLHDNWRFRQGRSDIWYPATVPGTVHTDLMANE
IIEDPFFRLNERGVQWVDKEDWMYETTFKATPEEVASENQQIVFKGLDTYADVYLNHECI
LRADNMHRTWTCDVKGKLREGDNLLEVYFNSPIKVDLPKYDKYDYTFNTGPDQSQNGGIF
NKTLSIFARKAGYHYGWDWGPRLVTSGIWRDIDLVTWNGLKIDNVQYVQKDVTAARANLS
TVVRVNSDTDVADATVTITADGKTVATKKTPLVKGLNTVTLDYTIKKPRLWWTNGLGEPY
LYGFSTSVKAGDDEVSQDEEIGIRSLRLITEDDEHGRSLAFELNGKPVFMKGVDMIPLDN
FLPRITRERYEKHVLDAKDVNMNMIRVWGGGVYEDDYFYQLCDRNGILVWQDFMFACSTY
PADSAFLANIRQEAIDNVERLRNHCSIALWCGNNECQDVYYGWGGRYNYYKEKGVEELTT
KQFKDMYFRTLPEVVEQCGGGISYRPSSPYAMEDTPSDGIHGDAHYWGVWHGRDSIGHYN
IEKARFFSEYGFQSFPEFESVKIYAPQERDWAINSEVMMAHQRAGSYANNLIKEYMDEEF
RTPEDFPSFLYVGSILQGDAIKTAVEAHRRDMPHCMGTLVWQHNDCWPVASWAGRDYYGR
WKAQQYYSKAAYDDILVSPVVINDTLSVNIVTDRRTPARGKFTLTAMTLDGKTVMSKTFD
FTAKPLTSTKVFSAGVAGLLDGRKKGDVIFHTTFETGGRTYENVGFSTKQKFMNYTAPDY
QIEVAAAGDGYDVTVGSDVFARGVFLSLDGIDNFFSDNYFNVMPGGKRTIHVTTPLGEKD
FRNQLKVISMGDIHARTEAGAAKGMKRDANFKPLGAN

Enzyme Prediction     

EC	3.2.1.25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	26	739	6.1e-104	0.6968085106382979

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.10e-101	27	681	13	638	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	8.57e-20	778	852	2	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10340	ebgA	9.61e-16	35	455	64	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam17786	Mannosidase_ig	1.65e-12	684	771	1	90	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam00703	Glyco_hydro_2	9.35e-11	222	324	3	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK63191.1	0.0	17	847	2	834
QUT31677.1	0.0	20	852	17	854
QGT72566.1	0.0	20	852	28	865
ALJ60279.1	0.0	27	852	31	863
QRM99606.1	0.0	20	852	28	865

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	8.36e-207	29	835	11	819	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	8.90e-207	29	835	13	821	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	9.18e-207	29	835	13	821	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.43e-206	29	835	11	819	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	1.05e-204	29	835	11	819	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B8NW36	6.84e-125	22	833	2	828	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1
Q2TXB7	2.63e-124	22	833	2	828	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
A2QYN2	1.12e-121	22	833	2	829	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
A1D911	1.60e-121	29	666	9	647	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
A1CGA8	6.13e-121	22	666	2	647	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000318	0.998962	0.000184	0.000186	0.000163	0.000155

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001738_00312.