CAZyme Information: MGYG000001748_00462

Basic Information

• Species: CAG-56 sp900762665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900762665
• CAZyme ID: MGYG000001748_00462
• CAZy Family: GH101
• CAZyme Description: Chromosome partition protein Smc

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2157		235526.81	4.5625

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001748	4218051	MAG	Sweden	Europe

• Gene Location: Start: 141911; End: 148384 Strand: +

Full Sequence      

MKKRCVTKYLSIVLAASMIATSAVPATALAAEVSVTADEETTGEDLGIEEDATEETEVTE
PVTETAAESALSEASASLVGASRAVYEDGAKDNWTQDEGTVLGEDAVNELVDGKRHLKAG
SVNGNAHNNPAQWPAVFVNPNEFDFTAEGFFEFTVHTKENPEHNRFGIFLGYSDPGNGMF
IGYDNYSKWYWQQYKDGGGTYTPIGGTNYPKENSSVHVKVSWTADKKVSVTVDDQKIIDA
LDCADFIVTNKIGFKCSTFNASSGKEITEVWLSDIHYTGQKEAAAYAVSGKVKDSKGNAV
AGAAVKLGNLSATTDNEGAYSFPQVADGSYTIQVTKYGYEPASTNVEVSGAAVSVPDITL
TETAPIETEILSTNDMDVYVAKHFPSVVKYQMKGNLDGKTFYGQQEELNTIVVNGVSFSI
DKADLTAVVDKTNKTATYVMNLKKEDATNPVDCVITAVLTAVDNTVSFDITEVKNNLTET
ELVAQATGDSIEQEKYPVQKIEIPNHSLISVNSTQTNANLKGAVTSSDTGVSGDSYYDVT
DDMTFKSGDYMYAFVSNDELSAGLWSNSEHTGSYAASPVRAAGGAGNTRIMATVTSNGDV
NTLGLASTEWYYNRKITTRLNQKTGDQISYVIEHTDMPNAKVAIAGDLNGDKSIDWQDGA
IAYRDIMKVPQGSEDVPNLVNQRIAENFASHAAEPFLMTLDGVKKVNLNTDGLGQSIILK
GYASEGHDSGHPDYGHVGERIGGVEDMNTLIAEGQKLGALFGVHINASEMYTEAKAFSND
LSAGNYGWNWLDQGIGINSYYDLGSGARWTRLNELKDAVGAGDFKTEPGLNYIYLDVWGN
NTTTNPEDSWESRQIAKQINDLGWRFTTEWGSTMEYDSTLQHWAADLTYGDASAKGQNSQ
VIRFLRNHLKDSWIADYQAYGGEAEAPLLGGLTMTDFEGWSGRTDYDNYITKMFRHNVIT
KFLQHYQVVKWENAPESDRYTFNHTLSGGGVHTHTWLPSKEITLRNTEDTSDDTELVVTR
KTTDHSNLADYRTRIITMNGVEISKGSVTSGDDTNPGTETYLIPWNWAADGTELSEADQK
LYHWNTKGGTTTWTLTDDWQNQASVVVYRLTEDGKTDKQTVNVVDKKITLTAEAETPYVI
YRGEKANTEMNWKSTKYITDTGFNDSAALTKYWTKTGTGTAGIAENMDANRMLKLDGEVA
VTSEKLTNLEAGEKYAVYVGVDNRSDARAYMSIKSGNKTLAYNYTGKSIVQNIVATDQHH
RASWSNEGGTTGDGYSYFQNMYVFFTAPEDTSNVTLVLGREAGKDGDKGSSYLDDIRVVP
TAMDADVEKDENGIITRLNQDFENSVQGIWPFVISGPGTPIYAVADNRIHLSEYNGEFTK
AGYRDKRVDDVLDGNWSVKINGLSSSDSMIYQTIPQNFHFEPGVTYHVSFDYQTGSDGAY
AVKVGEGSEIVDTLNMRGTTNLDESTGEYVAKTARYGFTFTAGESGQGWFGIYSTDVAPD
MKDAKAKPDDAQNFSGYRDFILDNLVIEKADFELSDTEISVTSAKDTVELKATVVNDVTL
DGPIEWTSDDESIAGVDENGIVTFYGYGTTMIKATASVNGVKQSLGCTVTLVKEVQSSGK
FVDVWANTEEPSGEPNGNGVAKTIIDGSGATYWHSNWASTGFEVSESNPAIVTVALSEGD
DISNYDTLTFQQRPSGKNGIIQKYECVVGDTFDQAKNTITGGTATGAVTVSDEGAKAGSV
VPVVLPEGTTGRYVQIRVLQGSGGFAAMAEITASYSLSYETPEEKVYLEKNRLTKQVKEQ
TAKAEELQAIIDNPEATEAEKEKAQEELSTLTPELEAAKTKLEEYTEYANLYQELEEAKA
DLEKAEGEKAELNKQLQQAQERLDKLTGEKAALEEELNKVKQELQDAKDQIASLKKEAED
AKKDAEDAKKEAEEAEQAKKEAEEALKKKEEEQKALEEALKRKEEEQKLQAEELKKKEAE
KKAAEEAAKKAEADAEQLKKQLQDAQDKLDKLNKGDNAIQKGDSVVYKNVTYKVLDADKK
TVAAVKAKNVASITIYGTVKVNGVTCSVTEIASNAFKGAKKLKKAVIGANVTKIGKRAFY
GAKKLRTVSIKTTKLKSVGSQAFGKIYVKAKITVPKSKKAAYKKLLKNKVLKKAVVK

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	374	1142	3.6e-272	0.9957567185289957

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	4.29e-142	657	950	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	5.57e-116	670	973	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam17974	GalBD_like	1.96e-72	1314	1516	1	190	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam18080	Gal_mutarotas_3	1.46e-71	371	656	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	3.78e-22	956	1116	1	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOL33684.1	0.0	94	1729	81	1636
BBK63058.1	0.0	85	1705	50	1598
BBK23316.1	0.0	85	1705	50	1598
QHQ54151.1	0.0	90	1720	66	1639
QUI44700.1	0.0	90	1658	66	1581

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEV_B	0.0	366	1531	7	1117	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
2ZXQ_A	0.0	366	1658	22	1263	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]
6QEP_A	0.0	366	1531	7	1117	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QFO_A	0.0	366	1531	7	1117	EngBFDARPin Fusion 9b 3G124 [Bifidobacterium longum]
6QFK_A	0.0	366	1531	7	1117	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	3.68e-256	114	1710	142	1568	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	3.68e-256	114	1710	142	1568	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	4.33e-101	348	1576	30	1081	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000341	0.998850	0.000230	0.000201	0.000183	0.000155

TMHMM  Annotations     

start	end
9	31