CAZyme Information: MGYG000001748_00463

Basic Information

• Species: CAG-56 sp900762665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900762665
• CAZyme ID: MGYG000001748_00463
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1992		217975.04	4.8171

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001748	4218051	MAG	Sweden	Europe

• Gene Location: Start: 148732; End: 154710 Strand: +

Full Sequence      

MKKRSVTKLLSIVLAASMIATSAAPATALAAEVSVTSDDENPGEGLGVAEETTVETEPAA
EIAEEQNVSEPSERIDRASRAVYEDGVIDNWTQDKNTVIGDEAVNELVDGTRYLKAGAQN
GNNHGNSEEWPAVFVNPNEFDFAAEGFFEFTVNTKENPKNNRFGIFLGYKDPGNGMFIGY
DDYSQWYWQQYKDGTGTYTPISGTSYPKENTSVHVRVSWTADKKVTVTVDENKIVDALEC
ADFIYTNKIGFKCSGFGSGSSKQLTEVWLSDIHYTGQKEVATYAVSGKVTDAAGAAIAGA
TVELNGNKATTAADGSYTLLEVPAGTHTVKVYKDGYETASQSITVQNAPLGNVVISLAEE
SSVGVQKLSTAEMDVFVAEDFPSVVKYLMKKGDLKDKVFYGQTEKIDTIKINGEAIKVTS
DNLKATFDAATGTYVMSLKKDSADNPIDCVITAVITAKDNTASFDITKVENKLTETEEVA
QADGSTKKQEKYPVQRIEIPNHSLLSVNSAQNNANMKGARTSSDTGVSGDSYVDVTDSMT
FKTGDYVYAFVSNDSMSAGLWSNSEHTGSYVASPVGAAGGAGNTRVQATVTDKGTAKTLG
LASAEWYYDRKISTRLNQKTGESVSYVVEHTDMPSAKVALAGEMNGDDTVNWQDGAIAFR
DIMNTPQGSEEVPELVNQRIAMNFGSQAANPFLSTLDGVKRVYLNTEGLGQAIILKGYGS
EGHDSGHPDYGDIGKRIGGADDMNTLIAEGQKLGAIFGVHINASEMYTEAKAFNNDLSAG
NYGWNWLDQGIGINSYYDLGSGARASRLKSLHDQVGDGNLTSETPGLNYIYLDVWGNGTT
TNGEDSWESRQIAKQINDLGWRFTTEWGSTMEYDSTLQHWAADLKYGNAAAKGQNSEVMR
FLRNHQKDSWIGDYGSYGGEAEAPLLGGLTMTDFEGWSSRTDYDEYITKMFRHNVITKFL
QHYQVVKWEDAPESDAYSFTHQINENTHTHSWVPTKEITLENTEDAADDTKIVVTRKSTD
HSNLNDYRSRTITMNGVEISKGAVTSGDDGIVGNAFQGDETYLIPWNWATDGSELSQNDQ
KLYHWNTKGGTTTWTLTDDWKGLSNVVVYKLTADGKTDKQTVNVVNNKITLKAEAEVPYV
VYKGEKANLTVDWQSTQYIYDTGFNDSAVLTNYWTTTGSGKAEIAENISANRMLKLNGEV
AVSSKALTGLTPKQRYAVYVGVDNRSSADAYMEIKSGGKRIAYNYTGTSYIANMVSSDQH
HTGSGGVEGRISYFQNMFVFFTAPETGEVTLTLGRRAGGDEGAVYFDDIRVVPTEMDADV
ETNADGIITKLNQNFENNVQGIWPFVISGPQQTEGWVTDNRIHLSEYNGEYTKAGYKDKR
VDDVLDGNWSVKINGLTGNRSMIYQTIPQNFRFEPGVTYNVSFDYQAGSDGTYELRVGDG
KNNNVTALNMPGTSQGQGEDAVTKTTRCSFTVTGSASGESWFGIYSTGNAADLKEFTGLD
DPKYNGKYNGQINFSGYKDFILDNLTIEKAGFTLSEDKVIARTAKDPIYLSVKALPGVEL
GKVTWSSENEDVAVVDEDGKVLFYNFGTTLIKATAIVNGQKQTVSCPVTLLEEETREARF
EKAWANTGAESVANIIDGELSTNWQSEYKTEDAVDADQFMVSTENPAVVTVQLPEGEDFS
NYNKLVFHERSDGLKGIVLYYECVFGSEFDENTHTIKNGVTTGRRQVESYAQSGVEKVEI
PEGANGRYAQIRVSGAYLEAAVIAEVYATGEFYYKTEEEEKNLIRNQMQRQLDDLQKQLD
AAKKEQEEAKAELERLKNASEAEKAAAQLQLKKAQEEAEKLRKELNDLKNKDMLKKGDTV
VYKNVTYKVLDPVKKTAAAVKAKNVTSIAIAKTVKVNGVTCSVTEIASNAFKGAKKLKKA
VIGANVTKIGKRAFYGAKKLRTVSIKTTKLKSVGSQAFGKIYVKAKITVPKSKKAAYKKL
LKNKVPKKAIIK

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	371	1143	9.1e-272	0.9957567185289957

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	1.37e-144	653	947	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	3.75e-118	666	970	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam17974	GalBD_like	7.31e-76	1307	1516	1	190	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam18080	Gal_mutarotas_3	9.74e-68	368	652	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	1.70e-22	953	1117	1	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK63058.1	0.0	63	1648	33	1558
BBK23316.1	0.0	63	1648	33	1558
QOL33684.1	0.0	91	1603	81	1499
QSY58793.1	0.0	66	1529	27	1430
QHQ54151.1	0.0	66	1529	45	1448

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEV_B	0.0	361	1529	5	1115	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
2ZXQ_A	0.0	366	1529	25	1130	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]
6QEP_A	0.0	349	1529	2	1115	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QFO_A	0.0	349	1529	2	1115	EngBFDARPin Fusion 9b 3G124 [Bifidobacterium longum]
6QFK_A	0.0	361	1529	5	1115	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	9.60e-260	110	1652	144	1528	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	1.34e-259	110	1648	144	1524	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	7.96e-103	368	1531	52	1038	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000300	0.998964	0.000185	0.000203	0.000179	0.000151

TMHMM  Annotations     

start	end
9	31