CAZyme Information: MGYG000001748_00583

Basic Information

• Species: CAG-56 sp900762665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900762665
• CAZyme ID: MGYG000001748_00583
• CAZy Family: GH39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1738	MGYG000001748_4|CGC3	191879.03	4.8852

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001748	4218051	MAG	Sweden	Europe

• Gene Location: Start: 142946; End: 148162 Strand: +

Full Sequence      

MKKQSIKKHSIKRWIAGILTAALVLPGAGVVPAGTAQAAESVPGLTVNMAPDEKRELYHG
ATGWLYGLGDDEVPTANTITALKPNTAVQKAPRGMQHPNGDVLDMANTFKNAGGKHIQIY
VPDYYALWFYEFTGTDYYLDILRMQAEECIKAGIAEDAVWVLYNEPNENWVGEYHDDQGN
KVSGWNSLYWFWKDMVEELREVYRENGITAPPKTAGLNLAVYNEGVMRDYMTFCKNHDCL
PDIISWHDLSTWQYNIFDREYNHYRSLEKSLGIEPREIVINEYADRAECASPGNLVRWIG
LWEDYQVAGCLPFWHLSNNLNGLAADTNEGNGAWWLYKWYGDMSGNYLPVSTSGAAQADF
YGAASLDENKKSANVIFGGQSGSSEIVLDQIDRTGTFKDTDKVHIKVEATDFTGFHGTAE
EPYVVKEGAVQVVDGRVSVPMSDMNALSAYRITVTQAAEDENAGLLSSTWKEMYEAEKGV
LAGNAKIVNADWQFACSNRQQVQNHNTGDSFTIDVKAPKEGYYRYDMLYGAATGVNTADP
ANHFPKTAVQKLIIDGGLAEEMVLQNTVNWSMEDMYSTYIYLTAGNHKITVEGSDSEGKA
VVDCIYLTYKGKTSYDTAFDKTYEAELGEFNEIKGETTKLTTKRDGAAGYITGLEARSVV
EGGGVRFNVVVPDNGMYRLALRYQAEEDAKANIYLDNDMVNLDNLRTTMELSAAGNTWRS
VYQNLFLQKGVNVVDIDTEGAVKLDSMSVSALEEESTPVVSVEAEDGTLAGTAAIGKSTG
TKTFASGNSYVAGMKAANGVELIEEEDPDFTILGLGRQKNEGRAVDANSLTLTVEAPEDG
TYKMVVYQSNGELFGKHDYNAQMTERFASFRVNDQEAQKVVFRNTYSDESFRPQVVNVNL
RAGTNTIKVYNDNSRVITNGVLKPGTSAHRPENIDYAALENYTPNFDKFEFYRVAGEAGE
VLEESSFAVNVNYTEGGIVDSDKETVNSGEDVRLTFVPNDGCVLVQAVANGQDITESLSA
AGGIYTLRNVRSTVNVEAYFTVSEESTELQREKDYEYAVNAGDVNPATLSEGDTFGVRNS
VTDQFYGGDPETGARWGVVDKYVSNNQYPDLLTGEKTWPCENDGATDTSPKGKSFRYARN
QPTTDVGVVYQFMLEPGQTYKAELGFYVPGSWTNASNPRTMKLVVNDTVVSGYEKFEASN
NSDAPYVISTQVTADENGDLKIQIGHADNAKWGPVVSYINIFRPVDTTSLENVLKDCEAY
HPGDYPKTEWDAFDQAYKAAEALVQDSSSLTDMVQINDAQYNLQTAVEKMVNAKNKVKLD
TLCSQYGDYAEEKGQGLTSEDDWYEFQDALLNAVYINGLEKISADQIARAEERLAKAAEK
LNELVNLEVKAPSKITYYVGEGLDITGMEVRAYGSQGGDPVILESGEYTIGDFDFSAPGT
KTIAVSYRGKTAEFTVRVAEAPVPTPELQGITATAVKTIYEQGEKFDPSTLYVTAHYSDG
SSKSVTDYQVSGFDSTKPGSIQITVTYQGKTASVSLTVKEKSAVEAKDIRQVKPSVKVTG
SSYSTVQIGWRKVEGAEGYQIYRAVSKNGTYRKVKTVKGGNTLSFTDQKKKFNKTYYYKV
RAYRTEKGKAVTSKFSNILAGKVKLEKPVVTLRKKTTTGMSVTWKRVAGASGYQVRYSLK
KKSGYKTATVNKGSRTKYMRKGLKKGKTYYVKVRAYRTVKGKKVYSPWSALQALSLKK

Enzyme Prediction     

No EC number prediction in MGYG000001748_00583.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04081	CBM35_galactosidase-like	6.62e-13	761	914	2	115	Carbohydrate Binding Module family 35 (CBM35); appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. This family includes carbohydrate binding module family 35 (CBM35); these are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. Examples of proteins which contain CBM35s belonging to this family includes the CBM35 of an exo-beta-1,3-galactanase from Phanerochaete chrysosporium 9 (Pc1,3Gal43A) which is appended to a GH43 domain, and the CBM35 domain of two bifunctional proteins with beta-L-arabinopyranosidase/alpha-D-galactopyranosidase activities from Fusarium oxysporum 12S, Foap1 and Foap2 (Fo/AP1 and Fo/AP2), that are appended to GH27 domains. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). Some CBM35s bind their ligands in a calcium-dependent manner. In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates, while family GH27 includes alpha-galactosidases, alpha-N-acetylgalactosaminidases, and isomaltodextranases.
pfam07523	Big_3	2.95e-10	1477	1538	4	67	Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
pfam07523	Big_3	8.28e-08	1392	1458	2	67	Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
cd04086	CBM35_mannanase-like	1.91e-07	762	912	3	107	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.
cd04086	CBM35_mannanase-like	6.00e-07	474	608	3	119	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBE97772.1	3.42e-187	6	1538	2	1719
QKE72773.1	4.71e-138	11	950	2	876
QKS45143.1	3.90e-127	39	985	673	1573
ASS97004.1	2.41e-120	45	954	35	973
QYR21026.1	5.14e-119	45	1033	35	1058

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U22_A	2.18e-50	29	457	13	423	Structureof N2152 from Neocallimastix frontalis [Neocallimastix frontalis]
2WZ8_A	2.87e-13	800	941	8	151	ChainA, Cellulosome Protein Dockerin Type I [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A401ETL2	4.18e-08	1384	1469	1373	1463	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000546	0.998435	0.000227	0.000311	0.000247	0.000208

TMHMM  Annotations     

start	end
13	35