CAZyme Information: MGYG000001748_00630

Basic Information

• Species: CAG-56 sp900762665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900762665
• CAZyme ID: MGYG000001748_00630
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1558	MGYG000001748_5|CGC1	174865.08	5.5952

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001748	4218051	MAG	Sweden	Europe

• Gene Location: Start: 56106; End: 60782 Strand: +

Full Sequence      

MQGKKKSLALLLAAAMVIGIPSPAVYGKTSEELAKGVQAQESSKVETTKCFNESWTEVTG
DGWRDGMISGNGENGVVTTGSPYGDRLIYQYMYFSFPSAATRFTPDETGYLDEARQAVFN
LDDSWNVHGRSPEYSYAFHPGHQLALNMAEKNSYSNYQRWTDYETAEVGVKYSDKNGDWQ
RKTFVSREDNVVITRIDKSSTGEKVNMTVSIDDIQSMSKSWDGMSTISKMQYKKLVDENA
DYIAEVGHYPAYDNSELKEGGFVGLTRVVTVGGQKERVLLQDTNEAINVGEDQNPAIKIT
DADAVYLVTKSKRTHTMGTYEEFRNQTQYDLLDELLNHINGVIDKEEYQTDGHFDYDKAL
APHAKLHGDQFNAVKFKLDGDEADKLLDNETLINKQKGERDRLNHAFIERAYNAGRYAQL
CCAGISAPRLYGMWTGEWQPGWRGIYTLDANVNIQVSPMNTGNMEDSALGYITFFLRNAP
DFEVNAEKIYGMHDAIQVPVNSDGERGILVEYYRDYPFQYWNSGASWTLLPIFEYWQCYG
NRQIPLTDMLDYQSLKSVLCVEDGGYTEEEMEAVIAKGYLDLEQDILLPLLTKQANFWEQ
FLTPEYYIDENGKACYQKGKTELEPGETYMLIPTYSPENHPRGYNSTITANSTMDIAAAR
DGLSMVIEMEKAVGRPGSKAAIEKWENLKSLLPKYKFDNTDSGKGALREWAMEEYIENND
HRHLSHLYLAWPGYETQNDEALFKAAKQAVANRNDYNYGDTTASHGWMHKALVNARLKDG
EGIITALKPLMTDNLYYTSMMGDHDTNRRNGTYCTDTIIGTVGVIQEAMVFSNTGEIEII
PALPEDWKSGSMDGILARTRAEVEKMSWNTEKGTANVQIRSDIDQTIRLKCGMEWSDAVV
SEPAKAEIEYSDYITLNLKQGDVVTVDFYNGDVTNGSLAEMIDAANSLLQQNKPDSQLYN
KRANEELQAVINAAKDAYFGENSDSKAIREAIWKLQDAMRQYEDSYRYQLVGSLQPGIYQ
KRQKLAIEHTQNDRMEVRYTLDGSEVTKDSCLYSGAVTLPTGKVSVRAAQFIKDTGVPVG
DSFEADYLIYANKNEALNKAAAINRNIYNGQGADKAVDGNAGTRLSVSGNYSDYILSVDL
GEPTVINQAYLDEYVETVDRETNRIQTFELQYQNLETGEWQTAYNFDGNNVGADMYLSRP
NPGGSSAHAYYGIKFDEITARQVRLVMTATKEISIWEFQLFNQREELGLASAIAKAESLL
ADEQEYDTQSWADMVAAYQKAKELFERTDVSQAELDTAVKELNSKVSALKRKEPETKKDF
TVSAPSSQVYTGKAITPAVTVKDQGKVLKAGSDYSLTYQNNVNVGSATIQVNGLNGYKDV
KKSVSFTITAYPAANLKIKADSKKVYTGKMIKPVPVVTHDNTKLREGRDYTVSYSGNKMI
GTAKITIRGIGNYAGSKAVTFTIIPKAPEKLKLKKSGANGITLRWKKVKGASGYYVYRST
AKNGKYRKVKTLNKAGMVSYRAANLKKNKKYYYYVKAFKKVKNKKIESNRSKVVIRKK

Enzyme Prediction     

No EC number prediction in MGYG000001748_00630.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	55	892	9.6e-127	0.9750692520775623

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	4.20e-15	55	308	2	224	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
pfam00754	F5_F8_type_C	1.22e-05	1106	1238	7	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam13290	CHB_HEX_C_1	8.22e-04	1016	1068	3	53	Chitobiase/beta-hexosaminidase C-terminal domain.
pfam07554	FIVAR	0.005	1249	1309	3	68	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
pfam13287	Fn3_assoc	0.007	1030	1071	5	45	Fn3 associated.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFH60346.1	0.0	51	932	20	903
QYR23103.1	0.0	51	929	16	902
AIQ45759.1	0.0	51	926	16	900
AIQ51360.1	0.0	51	933	16	907
QDH23244.1	0.0	59	928	28	901

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4UFC_A	1.18e-21	55	890	25	742	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]
7KMQ_A	1.75e-20	55	868	44	737	ChainA, Glyco_hyd_65N_2 domain-containing protein [Xanthomonas citri pv. citri str. 306],7KMQ_B Chain B, Glyco_hyd_65N_2 domain-containing protein [Xanthomonas citri pv. citri str. 306]
2RDY_A	2.19e-17	156	921	111	784	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
2EAB_A	2.20e-12	155	874	154	835	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAE_A	4.97e-12	155	874	153	834	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	2.65e-20	63	858	63	783	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	9.90e-11	107	869	86	757	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000243	0.999084	0.000171	0.000171	0.000153	0.000135

TMHMM  Annotations     

start	end
7	26