CAZyme Information: MGYG000001748_02984

Basic Information

• Species: CAG-56 sp900762665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900762665
• CAZyme ID: MGYG000001748_02984
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2342	MGYG000001748_44|CGC1	254873.49	4.5242

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001748	4218051	MAG	Sweden	Europe

• Gene Location: Start: 10913; End: 17941 Strand: +

Full Sequence      

MKKRLVNAGKKTAAVILSAAMAVSMVPAHLSFASETSDSNVSAKESTLELWYDDAAADSY
DGWMKSSLPLGNSAIGATVFGGVDRERIQLTEKSLWSGGPSDSRPDYNGGNLENYGQNGA
IMKQIQEAFANGETAKAQSLCNYLTGQSDDNGTEGYGYFLSYGNMYLDFSGVDSTKAENY
VRKLDLNTAVSTVEYDIDGVHYTREYFVSYPANVLVTKVSADKKGALDVDVSVLPDNEMG
NAVSSGGGKPTNSAYQRTWETEVKDGRITIAGQLTDNQMKFNSQTQVLNEGGTRADNDAD
GKVSVADADSITIITSIGTDYKNDYPKYRTGESDAQVSEKVAKYVDAAVKESYDALKQEH
IDDYYEIFGRVDLDLGQEIPQKDTQDLLKAYNEGKATEAERRYLEVLLFQYGRFLTVESS
RDNGGDEDRTTLPSNLQGIWVGANNSPWHSDYHMNVNLQMNYWPTYSTNMAECAEPLIDY
VDSLREPGRVTAKVYAGIESTDENPENGFMAHTQNTPFGWTSPGWVFTWGWSPAAVPWIL
QNCWDYYDFTGDVDYLKENIYPMMKEEATLYSQMLVEDEDGKLISSPAYSPETGPITNGN
TYEQSLIWQLFTDTITAGELVGEDEAILKEWQDILDNLKYPIEIGKDGQIKEWYIEEEFN
KDADGNKLGEGFTHRHMSHLLGLFPGDLVSVENQELMDAAIVSLNKRQDHSTGWGMGQRI
NTWARTGDGNRAYALITDLFNGGIYNNLWDSHAPFQIDGNFGMTSGVAEMLLQSNVGYIN
LLPALPDVWSEGQVEGLVARGNFEVDMAWSNGSLDNAVLKSNNGGEAVVQCDNAAYATVT
TDNGEYVSFDVLSNDRISFETTKGKTYYLGSLPKTQTISAPNGLAAERLTDDKVELSWNP
VEEENVTYTIYRQADNGSLQMIKEGVTETHFTDTSAYDFLGAMKYQIKAVAGEMESKLCD
QVEVNDLRNMAGMIDDRDSRLIYSSGWSTYAEPGLYNETSHFIENPVGNETVTFRFNGTG
LKVYATQNADRGIAEVWIDGVNKGDADSYAAGKKNFAEIYSITDLGKGIHTVELKVANRR
SNDAHTKTKFEFDAFEVLDDEATSVESVTVTSKTGMTVLSAANSTLQMQADVVTTGTNKK
VSWKVTDVNGVNTDLASIDDNGVLTVGDTNGTVRVTAVAKEDGRTSGYVDITIAIAGTET
ESNGTLVEFTRDGGLNTTDMTFSSDWYMWPEDGYLNGDKAESSTEGGTVTYTFVGTQFEI
YGGLNSTFGDFTVTIDDGTPIEATSWGEEDIKNTLLYRSSVLENKEHTIVITAKKREANN
KAKVALDYMMVYGAELVEFAVNNTANKEMGWTGNWSPYNADGPGDNEKNHHNGDKIDGTK
AGSTVSYSFTGTKFEIYGAKNKNFGEFTVTIDGNTSDKISTMVDGDYKDVLLYASDELSN
GRHSMVITTLSDKTVALDYMLIYKAPGEQVEIPDKSALQEAIAQQYKENYFKPSDAYTAG
WDAYQQAYVSAVEIMNKADAAEAEITGAIQALNTAAGNLMLRDDLPTPDLQNKEVSAGTV
ERDFIVLSWDVPEDADSILYYEVYEGDGTEPVQVTANNYCVLNGLEENHAYNYTVKAVNK
YGMISDYPAIQVKTKEAPDTTPPTPLEGLKRSGNTLSWNASTDESGSVHYEIWENGAMLE
RVQDTSYDITSLPEGSHTLSVAAVDGSGNRSQPASITFLKLNGKKDIASIFEPTALTVEK
GTEFSALELPKTVQAALGTADSGIVLSLGVTWDGTGYQKDQAGTYTLSGTLTLPEDTNIV
NTGNKKAAIKVTVAGEAEKNAIVAVQAVAPITVKYGTAFEKLSLPDQVEVTLESGNTQQV
NVQWAKGNYDASKTGTYTLSGTLKTTGNITNPDNKTASVTVKVEEYKITEVAASDKLTVE
EGTSFEDLVLPQTAKVTLDTGDTRDLAVEWVKGNYDGSKAGEYTLNGTVQVESGISNPNN
KTAAIVVKVTEKPVTVRKVVSVAEMDAVSAAYGTTFEQLTLPKTVKVTLDDGSSMDVNVE
WTNAGYDGTKEGEYTLTGKLKVPNNIDSNGKTATIVVKVSQKEVKAPEITAVASLGTISV
KKGTAFEQLALPKTVKATLDTGSTVDLSVQWAKGSYNANAAGSYTLTGTLVLANGITNPG
GKTASVIVKVTDDTQTIQKGKTYTSGNYRYKVTSTEKRTVTVMGPKSRNLKVIKVYSTVK
LGGKPYKVTAVAALAFKNCKKATSISIGANVKDIGKNAFYNCSKVKKAAISSKVLTKIGS
KAFYKCRKLGSITIKSTKLKSVGKQAFTGTAKKLVVKVPAKKRVAYRKLLKNKGLSKYAK
IK

Enzyme Prediction     

EC	3.2.1.63

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	45	835	3.2e-250	0.9875346260387812

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	6.70e-61	50	323	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
sd00036	LRR_3	1.50e-17	2235	2309	9	81	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
sd00036	LRR_3	2.78e-17	2235	2310	55	128	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
pfam13306	LRR_5	1.66e-16	2225	2310	19	101	Leucine rich repeats (6 copies). This family includes a number of leucine rich repeats. This family contains a large number of BSPA-like surface antigens from Trichomonas vaginalis.
sd00036	LRR_3	4.69e-16	2225	2321	22	116	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHB24557.1	0.0	1	1726	1	1570
QEI32060.1	0.0	1	1726	1	1570
QRT30709.1	0.0	1	1726	1	1570
ASM69030.1	0.0	10	1718	7	1557
QUO31342.1	2.09e-308	48	825	9	793

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	4.24e-216	63	827	47	847	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	3.04e-215	63	827	47	847	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	5.69e-215	63	827	46	846	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
2RDY_A	1.48e-119	63	814	14	737	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	1.53e-118	48	824	22	736	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	1.12e-95	67	814	66	797	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	1.25e-83	50	811	23	758	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	2.68e-78	58	818	34	784	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	2.34e-67	50	817	18	703	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2
P20533	1.30e-09	1541	1720	447	643	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000752	0.997980	0.000314	0.000380	0.000309	0.000235

TMHMM  Annotations     

start	end
13	32