CAZyme Information: MGYG000001750_01425

Basic Information

• Species: Phocaeicola ilei
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola ilei
• CAZyme ID: MGYG000001750_01425
• CAZy Family: GH63
• CAZyme Description: Glucosidase YgjK

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
640	MGYG000001750_29|CGC1	72913.98	5.1217

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001750	2839699	MAG	Sweden	Europe

• Gene Location: Start: 20041; End: 21963 Strand: +

Full Sequence      

MKTNIIKTLLLSGIISGFAACTTTSPKDMSYDRNRYEFNDILDIRLTPDSTVSRVGCFTD
AGSWMGFTLPQSDRWVNGFCGPFSIDNRIWFAQAAVEVGLSGKKDVMTPDSVSYFPGEVY
MASSSAEGRIFQRMNFIDANTALLQVETDCKAGLSFTARQWNSHIRLEAERNTVTAYHPN
GEIITLSFGNDITLTCDKHNYMAQTQPGCKKAVVAISFFTSEKELLANRQHIQFLLNNPK
EELAQNTARWNGYLQNVLRDDMKPEYDRIAVKSVVTLISNWRIHREGLLHEGVIPSHAIG
YFIGFWAWDSWRFSAALASFAPELAKNNIRAMFDYQLPDGMIIDCIYTNSAENNARDSKP
PLVCWAVDKIFTHTQDTAFVKEMYPQLLAYYKWWYVKRDHNHNGICELGSTDGTLEAAAW
ESGMDNAIRFDHTGMLKNAEDAWSMDQESVDLNAYLAYECRLLKKFASLAGTTFDGPDYS
DKIADYFFDEQTGWFCDRRLSDSSFIAEPGCEGYTPFWVGIADQRQMNKALVLLTDTTKF
STYIPFPTVAADNPKCDSNGYWRGPIWLDQTYQAITGLRNYGYKELADRYTEQVFERCQG
LKEDDPIHENYGTHDGKRLQAPHFSWSASHLLMMYEDYNK

Enzyme Prediction     

No EC number prediction in MGYG000001750_01425.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH63	195	638	3.9e-109	0.5701754385964912

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10137	PRK10137	4.71e-117	1	638	1	783	alpha-glucosidase; Provisional
COG3408	GDB1	1.74e-14	292	635	271	603	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
pfam01204	Trehalase	1.21e-12	293	639	119	509	Trehalase. Trehalase (EC:3.2.1.28) is known to recycle trehalose to glucose. Trehalose is a physiological hallmark of heat-shock response in yeast and protects of proteins and membranes against a variety of stresses. This family is found in conjunction with pfam07492 in fungi.
pfam03200	Glyco_hydro_63	1.69e-08	377	636	185	494	Glycosyl hydrolase family 63 C-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the C-terminal catalytic domain.
COG1626	TreA	3.35e-04	418	617	324	529	Neutral trehalase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY37311.1	0.0	1	640	1	638
QJR62245.1	0.0	1	640	1	644
QJR74523.1	0.0	1	640	1	644
QJR66502.1	0.0	1	640	1	644
QJR61716.1	0.0	1	640	1	644

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W7S_A	2.81e-75	54	638	28	756	Escherichiacoli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7S_B Escherichia coli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7T_A Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7T_B Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7U_A Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12],3W7U_B Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12]
3W7X_A	1.44e-74	54	638	28	756	Crystalstructure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],3W7X_B Crystal structure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],5CA3_A Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5CA3_B Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
3W7W_A	2.00e-74	54	638	28	756	Crystalstructure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],3W7W_B Crystal structure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],5GW7_A Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5GW7_B Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
3D3I_A	2.69e-71	54	638	29	757	Crystalstructural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12],3D3I_B Crystal structural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12]
7PQQ_B	4.93e-33	400	638	21	305	ChainB, Anti-RON nanobody,Megabody 91,Glucosidase YgjK [Lama glama]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P42592	2.28e-74	54	638	51	779	Glucosidase YgjK OS=Escherichia coli (strain K12) OX=83333 GN=ygjK PE=1 SV=1
D8QTR2	4.14e-17	306	629	98	479	Mannosylglycerate hydrolase MGH1 OS=Selaginella moellendorffii OX=88036 GN=MGH PE=1 SV=1
D8T3S4	9.72e-17	306	629	98	479	Mannosylglycerate hydrolase MGH2 OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_447962 PE=3 SV=1
O43280	1.73e-08	290	638	160	550	Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2
P53008	1.43e-07	377	628	538	824	Mannosyl-oligosaccharide glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CWH41 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000044	0.009063	0.990909	0.000006	0.000009	0.000006

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001750_01425.