CAZyme Information: MGYG000001761_00213

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger
• CAZyme ID: MGYG000001761_00213
• CAZy Family: GH3
• CAZyme Description: Xylan 1,4-beta-xylosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
867		96049.2	4.6911

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001761	2495136	MAG	Finland	Europe

• Gene Location: Start: 9373; End: 11976 Strand: -

Full Sequence      

MFPYQDAKLPIEERIDDLLGRMTLREKILQTDQYFSGDFTAQDENGQVTAMDLDRLDALL
QGCSVGSVQLRGMDAAQANTVQRYAMEKTRLGIPFLFSEEALHGLMTNRATSFPQQIGLA
ATFDPELGRRMGHAIATETRAMGIHETYSPVMDLIRDPRYGRGEESYGEDTHLCGEFARE
VVRGMQGDDLTAPDAIAAEPKHYVGYGNPVGGLNCAPCTMGRHDVFSDCLPVFEAAFRDG
GAVDAMCSYNSIDSIPVSMDHELLTDVLRGQYGMRGFVRTDLTAVSRLYDWHFIAPTPEE
AIRLGVEAGVDLQLYDFPHDVWQDSLQHLVESGRMDVSVVDTACRRVLRVKFMLGLFEHP
YTDEHRAETVLRCPDHLELARRIARESVVLLKNDDLLPLRKDLDCIAVIGPGADEAALGD
YSEARGRTGQVTVLDGIKAAVSPKTKVLHARGCNFLGQALHPFDPGMLQDEEGNAGLTGR
YYNGPRPEGTPVQTRNDRTVNFNWIFALPHPELDAHCFSVAWTGYVCMPKTMDGCIGLST
QDSMRLYIDDELVIDGWGPDKSADQALDFHFEAGHRYKVRIEFTNDRRGARVIFGYSAGR
EDFDAAVAAARQADVAILCMGDNEETSGENFDRTSLDLPGRQLELVKAVYETGTPVVLLL
QSGRPVSANWENEHLPAILEAWFPGEQGGAGIADVLFGDAGPSGRLPITFPRSVGQVPCH
YSRRPGGGKRYVEMDWLPLYPFGYGLSYTTFAYRDLTLDRTEMDPGDTVQVSFTVENTGT
RPGVAVPQVYVRDYFSSVVKPERQLAAFTRVELAPGESRRVSLPVGTRALRTLGPDYVWR
VEPGDFEIQLGDNAENILLRTDLTVRG

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.37

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	86	314	1.6e-60	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.08e-170	3	851	23	751	beta-glucosidase BglX.
COG1472	BglX	1.92e-79	22	454	1	396	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	4.77e-67	2	852	40	770	Probable beta-xylosidase; Provisional
pfam00933	Glyco_hydro_3	1.40e-58	23	350	1	316	Glycosyl hydrolase family 3 N terminal domain.
pfam01915	Glyco_hydro_3_C	2.24e-52	388	748	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCJ98992.1	0.0	2	865	9	869
QAY68112.1	5.94e-297	4	865	9	876
BBI31696.1	3.16e-289	4	865	11	879
AXP81467.1	5.54e-239	3	865	25	879
APA00260.1	3.01e-234	3	858	30	878

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XXL_A	3.28e-130	5	857	4	744	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	1.79e-129	5	857	4	744	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5YOT_A	6.43e-127	4	857	7	748	Isoprimeverose-producingenzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]
7EAP_A	4.89e-126	4	857	7	748	ChainA, Fn3_like domain-containing protein [Aspergillus oryzae RIB40]
5TF0_A	9.90e-125	6	857	2	743	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56078	3.41e-121	15	857	38	757	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	8.34e-117	15	857	38	757	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
T2KMH9	1.30e-116	4	854	29	744	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
T2KMH0	7.49e-111	90	866	62	723	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
P27034	1.35e-89	90	857	31	802	Beta-glucosidase OS=Rhizobium radiobacter OX=358 GN=cbg-1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000090	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001761_00213.