CAZyme Information: MGYG000001763_00453

Basic Information

• Species: Prevotella sp000434975
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp000434975
• CAZyme ID: MGYG000001763_00453
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
777	MGYG000001763_6|CGC1	86937.05	6.3716

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001763	2771387	MAG	Denmark	Europe

• Gene Location: Start: 30128; End: 32461 Strand: -

Full Sequence      

MKKLFSSIFMLALAYTASAKVPVIKSDPKIEAQVEQTLKKLTLEEKVGQMMELVTDLFGA
NDKNGVFYIDEHKTDSILSRYKIGSILNAPNTCAPTAKQWEKYIAQIQKISMKRIGIPCV
FGLDQNHGSTYTQDGTLFPQNINVAATFNREIARRSAEATAYETRAVSVPWTYSPTVDLG
RDARWPRIWENFGEDCYLSSEMGKAMVYGFQGEDPNNIDQYHIATSMKHFMGYGVPWTGK
DRTPAYISPSDLREKHFAPFLAGLQAGALTVMVNSASVNGLPMHANKEILTGWLKEQTGW
DGVLVTDWADVNNLYTREMIAKDKKDALRIAINAGIDMIMEPYSCDACGYLIELVKEGKI
PMSRIDDACRRVLRMKYRLDLFKNPTQKLKNYPKFGGEEFAKLALEGATESMVLLKNEKL
QDGNPVLPLAKGKKILLTGPNANQMRCLDGGWSYTWQGHRTDEFAGKYNTIYEAFCNQYG
KENMVLNQGVTYNEKGKYWEENEPQIQGAVDAAKNVDVIVACIGENSYTETPGNLTDLWL
SENQRNLVKELSKTGKPVILVLNEGRPRLIADIEPLAQGIIDILIPGNMGGDALVNLVSG
KSNFSGKMPYTYPKEINSLANYDFKKSEEVGTMEGAYDYNAKITQQWGFGHGLSYTSYKY
SNLKVSQSDFRHGDIIKVSVDVKNTGKVAGKESVLLFSSDLIASMVPDGRRLRAFDKIEL
QPGETKTVTFNLNADDLAFVGYDGKWVLEEGDFKLMIADQSADIHCDKTYQWQSANR

Enzyme Prediction     

EC	3.2.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	112	340	3.3e-65	0.9768518518518519

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.37e-141	1	765	1	758	beta-glucosidase BglX.
COG1472	BglX	7.48e-84	41	455	1	374	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	1.14e-72	42	375	1	316	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	1.53e-55	101	759	64	770	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.53e-42	412	655	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNT65078.1	0.0	1	777	1	772
QUB87534.1	0.0	1	777	1	772
AKU68581.1	0.0	1	777	1	772
QKH87834.1	0.0	1	777	1	772
QUB63923.1	0.0	1	777	1	772

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JP0_A	0.0	22	777	5	759	Bacteroidesovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus],5JP0_B Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus]
5TF0_A	3.54e-102	25	764	4	743	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]
5XXL_A	6.33e-101	25	764	5	744	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	3.36e-100	25	764	5	744	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
6R5I_A	1.06e-96	82	762	45	723	ChainA, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5I_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXU3	0.0	14	777	22	781	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
Q23892	9.94e-136	34	765	80	819	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
P33363	3.31e-111	31	764	35	757	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
Q56078	9.58e-110	31	764	35	757	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
T2KMH0	1.68e-96	113	761	61	710	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000394	0.998833	0.000207	0.000189	0.000184	0.000173

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001763_00453.