CAZyme Information: MGYG000001769_00628

Basic Information

• Species: CAG-488 sp000434915
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; CAG-488; CAG-488 sp000434915
• CAZyme ID: MGYG000001769_00628
• CAZy Family: GT2
• CAZyme Description: Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
879		104685.15	6.54

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001769	2247196	MAG	Denmark	Europe

• Gene Location: Start: 102078; End: 104717 Strand: +

Full Sequence      

MDFEYQASVIVPVYNVEQYLRICLDSLVNQTIDKKRMEVLVINDGSPDNSWDICREYSEK
YDFIKIFSKENEGLSATRNFGIKHAKGKYMFFLDSDDYFTPETVKKVTDFFDKVYDEVDL
VTYNETRYRDDVILKPHFRFKMLDHKGVYDLNEYPYLTQTRVNVCVKNMGEKNVLFNTTP
GFKLEDQEYCNRVLMAKMKMGYCPHGTYMYNKGNETSIMKMYFHAFYLFETSMEYFENLF
ATFPDKVPPYFQAMFVNDINWRLSDDILYPYHYEGEKFEKAMRRIVDLLNRVDSKTILEH
PMVDVYRACYWIKKKENAEIAFDCTDDGILLTVDGEEVYSQEDLVVIGHKPRVEGDIFRL
TAFMKSPIFTFLNDDEYAIYAVENGKRKKLDLFTSVHSNYKAKTITNPFPAFHYSCDLKE
NNKIRFEIDIKGHTYSSHYWFMQYSGFGLRFIRKINRGKYMIKNKKKYLSVKPATQREID
FNAIFNTARFAIHPRTAMLRLKSIDYRKKHRIWLYYDFYTVEKDNGYYQFINDLKHDDGV
ERYYVLTHEYDDLNKLFTAEQQKHLVKAGSKEHRLLFICAERIFTAYYGLAPVNPFETAK
MEYQYNDLIKSRTIYLQHGVLHASLRLLNSEERSQAEEIVVSSPFEVKNYMQNYAYEKRE
LITTGMARYDHIDRNHKPQNRILFAPSWRKYLTTEVKASNWEANVEKIQKSDYYKNFYKF
LSDKNLHKKLESSKVYLDIKLHPIIANLTDLFDIDCEYVNMVGDVEVADYKAFVTDFSSF
VFDFAYLNRPVMYFVPDYPQFKSGMNHYRDLDLPFEDAFGHMFTDAHKAAECLCDIIDRD
FVPEEPFGERMKNFYFDFDGNCAENLYKFVTEQDKEFSK

Enzyme Prediction     

No EC number prediction in MGYG000001769_00628.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	8	138	1.1e-34	0.7647058823529411

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00535	Glycos_transf_2	1.08e-33	8	140	1	130	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
cd00761	Glyco_tranf_GTA_type	1.37e-30	9	122	1	112	Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.
PRK10073	PRK10073	1.95e-24	8	131	9	128	putative glycosyl transferase; Provisional
COG0463	WcaA	4.75e-21	8	291	6	273	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
cd02525	Succinoglycan_BP_ExoA	1.02e-20	8	132	3	126	ExoA is involved in the biosynthesis of succinoglycan. Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANE23153.1	1.80e-172	8	870	8	878
QUN13088.1	1.82e-170	2	872	3	873
QEO63827.1	1.48e-164	1	874	1	870
QCU20573.1	2.08e-164	1	874	1	870
QFK72898.1	1.98e-162	3	867	2	864

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HEA_A	3.09e-22	1	98	1	96	CgTstructure in hexamer [Streptococcus parasanguinis FW213],5HEA_B CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEA_C CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEC_A CgT structure in dimer [Streptococcus parasanguinis FW213],5HEC_B CgT structure in dimer [Streptococcus parasanguinis FW213]
3BCV_A	4.17e-18	6	98	6	96	Crystalstructure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3BCV_B Crystal structure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5TZE_C	3.12e-14	8	121	4	118	Crystalstructure of S. aureus TarS in complex with UDP-GlcNAc [Staphylococcus aureus],5TZE_E Crystal structure of S. aureus TarS in complex with UDP-GlcNAc [Staphylococcus aureus],5TZI_C Crystal structure of S. aureus TarS 1-349 [Staphylococcus aureus],5TZJ_A Crystal structure of S. aureus TarS 1-349 in complex with UDP-GlcNAc [Staphylococcus aureus],5TZJ_C Crystal structure of S. aureus TarS 1-349 in complex with UDP-GlcNAc [Staphylococcus aureus],5TZK_C Crystal structure of S. aureus TarS 1-349 in complex with UDP [Staphylococcus aureus]
5TZ8_A	7.84e-14	8	121	4	118	Crystalstructure of S. aureus TarS [Staphylococcus aureus],5TZ8_B Crystal structure of S. aureus TarS [Staphylococcus aureus],5TZ8_C Crystal structure of S. aureus TarS [Staphylococcus aureus]
6P61_A	3.31e-13	8	150	16	168	Structureof a Glycosyltransferase from Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) [Leptospira borgpetersenii serovar Hardjo-bovis str. JB197],6P61_B Structure of a Glycosyltransferase from Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) [Leptospira borgpetersenii serovar Hardjo-bovis str. JB197],6P61_C Structure of a Glycosyltransferase from Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) [Leptospira borgpetersenii serovar Hardjo-bovis str. JB197],6P61_D Structure of a Glycosyltransferase from Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) [Leptospira borgpetersenii serovar Hardjo-bovis str. JB197]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P46918	1.05e-59	2	874	5	894	Minor teichoic acid biosynthesis protein GgaB OS=Bacillus subtilis (strain 168) OX=224308 GN=ggaB PE=3 SV=1
P71057	1.69e-20	8	124	7	118	Putative glycosyltransferase EpsH OS=Bacillus subtilis (strain 168) OX=224308 GN=epsH PE=2 SV=1
P46917	1.76e-20	8	319	3	324	Minor teichoic acid biosynthesis protein GgaA OS=Bacillus subtilis (strain 168) OX=224308 GN=ggaA PE=3 SV=1
A0A0H2URH7	4.38e-20	8	110	8	112	Glycosyltransferase GlyA OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyA PE=3 SV=1
P71059	7.99e-19	8	109	6	105	Uncharacterized glycosyltransferase EpsJ OS=Bacillus subtilis (strain 168) OX=224308 GN=epsJ PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000069	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001769_00628.