CAZyme Information: MGYG000001777_00056

Basic Information

• Species: Eubacterium_F sp000434115
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_F; Eubacterium_F sp000434115
• CAZyme ID: MGYG000001777_00056
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1180		133467.51	4.5069

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001777	2510144	MAG	Denmark	Europe

• Gene Location: Start: 66069; End: 69611 Strand: +

Full Sequence      

MKKRLNDNWTFCKFPLDTPTETLFQITSWTPVDLPHDWMIYDTENLYEQAVSCYRRMINK
KQLLDGAAPDTQKIELIFEGVYMDTTIYLNQKKIFTWKHGYSEFVVDLTDELQDGDNELL
VRNEYHLPNSRWYPGSGIYRDVWLLTSPAAHLVHNGTYLSTKQLGKTWEVFMDTEFIDEK
GTASDGRICHEIFDADGTLVTTHESSIHFSDSVQVDKQVLTVIEPKLWDIEEPNLYQIRT
SLYVGDTLTDSITQNLGFRDIAFDADKGFFLNGRHVKINGTCEHHVLGSLGAAMNKAALR
RQLLTLKEMGVNSIRNAHNMPPEHMLNLCDELGLLLYTESFDMWERTKTDYDYGNDFKDW
WKKDLTSWVRQDRNHPCVFIWGIGNEIYDTHFDRGLELTKELHNAVRELDYRKNAYTTLA
SNYIEWEAAQRTGEVIDLVGYNYLDKCYDEHHEKYPDWKIFGSETSATVQSRGIYHFPLS
NRLLTFDDLQCSTLGNCTTNWGAKNSEHTIIAHRDREFCFGQYLWSGFDYLGEPTPYFTK
NSYFGQVDTAGFPKDTFYLYQAEWTDYKKKPMVHLLPYWDFNEGQLIDVRIFSNAPKVEL
FYQGQSQGIVEIDHAHGTTLSGDWQIPYSKGMIEAVAYDEEGNVIARDRQQSFGDPASIR
LTPDKETLTADGEDLIFVTIDTLDANGILVANARSRMNVSVTGAGRLIGLDNGDSSDFDQ
YKGTSRKLFSGKLLAIIAAKTESGDIQVTVSSPSLPDAHLTLQAVPAEIVPDGISCQMEN
TTYSLAEKTLESGILIPDRTNEIPVRKIELTNQGTSILDAEHKETVVTAKIYPENATYRD
IEFKAVTLNGVQSNAVKITVDKETNTASIQAVGDGEFRLCCTCCNGLDHVEVISELEFEV
TGLGDATHDPYKIVSAIDYSESSDDGLILSFQGGVYITGNDRTVVTFENIDFGDYGSDEI
HLPIFSFSDVLPVEVWLGTPGLPSEKGSITEPAVPGTTCATGECLLQATYEAKSWYNHYQ
ENVFTLSKRLKGVQTISIVVYPEIKMSLQGFYFTKVEKAYAKLLGSDCANVTGDMFTIDG
DAIREIGNNVTVEYTNMHFTKDGFHQITICGRSHIEMNTIHVRFVPADGSDAVINQIVDF
PYSGESQVQTFELEPVSGDYNLNLIFLPGCKFDLEWLQFE

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	560	1.8e-98	0.5917553191489362

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.47e-63	1	580	11	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	8.51e-40	659	761	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK09525	lacZ	1.39e-30	2	386	52	462	beta-galactosidase.
PRK10150	PRK10150	5.14e-28	54	410	69	440	beta-D-glucuronidase; Provisional
PRK10340	ebgA	8.72e-26	70	386	124	449	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNO17150.1	0.0	1	1180	1	1136
BCJ98579.1	0.0	1	1179	1	1135
BCJ94036.1	0.0	1	1180	4	1146
CQR57247.1	0.0	1	1179	3	1154
ABX43515.1	0.0	2	1180	9	1185

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	1.15e-173	6	762	7	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.50e-172	6	755	13	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5DMY_A	9.77e-145	8	766	44	886	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]
6QUB_B	3.03e-143	8	766	15	857	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]
6QUB_A	6.40e-143	8	766	38	880	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	8.28e-154	24	764	66	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	9.05e-91	1	752	47	808	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	2.84e-58	2	637	53	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	5.25e-42	6	709	9	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	4.92e-39	6	818	32	855	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000065	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001777_00056.