CAZyme Information: MGYG000001780_02380

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000001780_02380
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
865	MGYG000001780_15|CGC1	100245.53	6.6721

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001780	6261722	MAG	Denmark	Europe

• Gene Location: Start: 39498; End: 42095 Strand: -

Full Sequence      

MKKPDSNLKRVSLLTSSLLCSALLIAVPLRKELNEGWKFKQARLSNWYPATVPGVVHTDL
MDNKIIEDPFFRLNERGMQWIDKEDWIYQTTFQLTPEMMGRENIDLIFKGLDTYADVYLN
EKKILEANNMFREWKTSIKPDLKPGENILKIYFHSPIKVDIPKWDALPYQYEAGNDQSEN
GGVFNKKVSVFARKAGYHYGWDWGPRLVTSGIWRPVYVEAWDNARINDVFIRQPEVSKSR
ASLIGEVEILADKEIDQANVTITEAASGRVLAGQAISLQKGINKISLPFSIKNPKLWWSN
GLGEQHLYSFRTALTVNNQTSDVRTEEVGLRSLKIINRPDKDGKTFYVELNGIPVFAKGA
NYIPQDNFLPRVTPEQYEKTILDAANANMNMLRIWGGGTYESDLFYRLCDRYGILVWQDF
MFACSLYPAEGELLENIRQEAIDNVKRLRNHACIALWCGNNECNDAWFNWGWQKRYKAQN
PEYEQKIWKQFNDQYNVTLPQVVEEYAPESFYWPSSPFARYDGGSDDRNGDRHYWEVWHG
KKPIDMYNKERSRFFSEYGFQSFPEFESVKRYAPRQEDWDIYSEVMMSHQRGGMHANELI
ETYLLNEYRKPRNFEAFLYMNHVLQGDAIKTAIEAHRRDMPYCMGTLFWQHNDCWPVASW
ASRDYYGRWKAQHYFARKAYRDILVSPIADEDGQLKVQIVSDRHKVCNGRLEVKVMKLTG
EVLNSYKRNVKVDANSSKALFSVPLDEVLKGARKEDVFIHAVLLTDKGNSNYTNNYFLVK
QKEVNYPKAQLATSVQPIEGGFEVTLSSDNFARAVFIATGDANSSFSDNYFDILPGSSVK
VEVYTDLPLATFEKQLKVMSLSDEY

Enzyme Prediction     

EC	3.2.1.25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	30	826	1.4e-109	0.7805851063829787

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	9.29e-118	30	842	12	783	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	6.16e-21	788	863	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam17786	Mannosidase_ig	2.32e-20	693	783	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
PRK10150	PRK10150	1.07e-15	30	462	12	417	beta-D-glucuronidase; Provisional
PRK10340	ebgA	5.42e-13	33	464	44	455	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO68488.1	0.0	1	865	1	865
QUT88714.1	0.0	1	865	1	865
ALJ60279.1	0.0	1	865	1	865
QUT74423.1	0.0	1	865	1	864
QUT31677.1	0.0	26	865	19	856

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	9.41e-243	33	841	11	814	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.00e-242	33	841	13	816	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.04e-242	33	841	13	816	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.75e-242	33	841	11	814	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	1.21e-240	33	841	11	814	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A2QYN2	3.32e-132	33	843	9	828	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
Q0CCA0	1.87e-130	30	685	6	656	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
A1CGA8	2.75e-130	30	685	6	658	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q5B7W2	1.01e-129	33	843	9	826	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
Q4WAH4	6.10e-128	33	685	9	658	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.800075	0.191753	0.004048	0.000875	0.000553	0.002695

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001780_02380.