CAZyme Information: MGYG000001783_00037

Basic Information

• Species: Phocaeicola sp900551065
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola sp900551065
• CAZyme ID: MGYG000001783_00037
• CAZy Family: CE2
• CAZyme Description: Cellulase/esterase CelE

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
369	MGYG000001783_1|CGC3	41557.75	6.9894

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001783	3080501	MAG	Denmark	Europe

• Gene Location: Start: 45221; End: 46330 Strand: -

Full Sequence      

MRNNRFLFCFALSAILLGGFLPIWAGCLPPSKDFVSSDDENIVYMGRISKRIPHAVRFTY
PGVSIFANFEGTSLQMLTSPGCGSFMVEIDEQLPFRINFAEKDSVQTLAEGLLAGVHRVR
IMYVVEGYELKPSFKGFYLDKGCRLAEAPVLPERRIEFIGNSITCGYGVESENPSDPFSY
ETENHFYTYAARTARALQAQHLVVARSGIGIYRNYGAPREGSKDCLPAMYEQTLFMDSTE
HWNHALYTPDVVCINLGTNDTSLDNYDMHLLTEGYRGFMKRLRAIYPKAKLVLLTGSMMN
GKPLEDVKKAMDTVVREMHAKGDGVVYRFDMSPQTGSLGIGASYHPSMRQHQKMACELTA
FLKDLMKWE

Enzyme Prediction     

No EC number prediction in MGYG000001783_00037.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE2	155	362	1.1e-63	0.9952153110047847

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01831	Endoglucanase_E_like	1.50e-41	155	363	1	169	Endoglucanase E-like members of the SGNH hydrolase family; Endoglucanase E catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
pfam17996	CE2_N	2.63e-32	44	149	1	108	Carbohydrate esterase 2 N-terminal. This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum.
pfam13472	Lipase_GDSL_2	3.44e-12	158	353	1	176	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
cd00229	SGNH_hydrolase	2.39e-11	156	355	1	181	SGNH_hydrolase, or GDSL_hydrolase, is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.
cd01827	sialate_O-acetylesterase_like1	1.93e-06	155	355	2	180	sialate O-acetylesterase_like family of the SGNH hydrolases, a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY36201.1	1.20e-187	23	368	24	369
QNT66876.1	1.91e-119	28	368	29	370
QCD38714.1	1.62e-115	35	364	31	357
QCP72402.1	1.62e-115	35	364	31	357
QCD41872.1	5.85e-113	23	366	19	359

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAO_A	1.49e-48	36	368	3	333	ChainA, ENDOGLUCANASE E [Acetivibrio thermocellus]
2WAB_A	4.11e-48	36	368	3	333	ChainA, ENDOGLUCANASE E [Acetivibrio thermocellus]
2WAA_A	1.50e-33	28	368	6	339	Structureof a family two carbohydrate esterase from Cellvibrio japonicus [Cellvibrio japonicus]
2W9X_A	2.26e-26	53	364	45	355	Theactive site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus],2W9X_B The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus]
4XVH_A	3.74e-23	41	368	1	329	Crystalstructure of a Corynascus thermopiles (Myceliophthora fergusii) carbohydrate esterase family 2 (CE2) enzyme plus carbohydrate binding domain (CBD) [Chaetomium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P10477	3.19e-45	31	368	479	814	Cellulase/esterase CelE OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celE PE=1 SV=2
B3PIB0	1.01e-32	28	368	25	358	Acetylxylan esterase / glucomannan deacetylase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=axe2C PE=1 SV=1
B3PDE5	1.13e-25	53	364	45	355	Acetylxylan esterase / glucomannan deacetylase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=ce2C PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000024	1.000008	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
7	26