CAZyme Information: MGYG000001798_00259

Basic Information

• Species: Ruminococcus_D sp900752625
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_D; Ruminococcus_D sp900752625
• CAZyme ID: MGYG000001798_00259
• CAZy Family: GH5
• CAZyme Description: Endoglucanase A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
427		47749.48	3.949

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001798	2367577	MAG	Denmark	Europe

• Gene Location: Start: 14614; End: 15897 Strand: -

Full Sequence      

MKKAKIILIMTALTLALGSFAGCAGNDVSSDAETGTGPAETSGGENAESIDLNRPGEVRD
ITPAELVAEMKTGWNLGNSLDSEAAGLEAETAWNNPKTTKEMIDAVVAKGFDVIRIPVTW
NEHMGEAPEYTVDVEWMDRVQEVVNYAIDNDVYVIITAHHEEPWRIPDYEHIDAVDEQHQ
ALWRQIAERFKDYGDKLIFDGLNEPRIEGGANEWNGGTEEGRRCIDRLNQSFIDVVRSTG
GNNETRLLLVTTFATQAVSQAINDVAIPDDDHVAFSIHAYTPYAFTYHSNESWELYNWDG
THTYEIENMFSGLKTAFLDKGVPVIITEYGAVSKFYDDEQQEETGELINEKEVVKWVTDY
LTIAKSYGIPCVWWDNNEYISNGELFGIFNRDDLTWFTDEVPDAIMAVYDEDSTDAAETA
DSADTAE

Enzyme Prediction     

EC	3.2.1.4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	90	377	1.7e-95	0.9891304347826086

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	6.22e-67	86	377	12	269	Cellulase (glycosyl hydrolase family 5).
COG2730	BglC	4.69e-30	63	413	44	394	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAA38692.1	1.59e-168	49	406	13	360
QPB75692.1	5.71e-168	51	406	61	406
ADU23111.1	2.31e-167	51	406	61	406
AAA26467.1	2.20e-161	1	405	1	409
AOZ95201.1	1.51e-157	2	409	4	416

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4W85_A	1.33e-104	65	406	7	336	Crystalstructure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose [uncultured bacterium],4W85_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose [uncultured bacterium],4W87_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with a xyloglucan oligosaccharide [uncultured bacterium],4W87_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with a xyloglucan oligosaccharide [uncultured bacterium],4W89_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with cellotriose [uncultured bacterium],4W89_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with cellotriose [uncultured bacterium]
4W86_A	8.57e-103	65	406	7	336	Crystalstructure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose and TRIS [uncultured bacterium],4W86_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose and TRIS [uncultured bacterium]
4W84_A	8.86e-103	65	406	7	336	Crystalstructure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in the native form [uncultured bacterium],4W84_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in the native form [uncultured bacterium],4W88_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with a xyloglucan oligosaccharide and TRIS [uncultured bacterium],4W88_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with a xyloglucan oligosaccharide and TRIS [uncultured bacterium]
6WQP_A	1.97e-102	52	406	5	353	GH5-4broad specificity endoglucanase from Ruminococcus champanellensis [Ruminococcus champanellensis],6WQP_B GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis [Ruminococcus champanellensis],6WQV_A GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_B GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_C GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_D GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis]
6MQ4_A	6.42e-95	58	406	7	346	ChainA, cellulase [Acetivibrio cellulolyticus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P23660	3.19e-169	49	406	13	360	Endoglucanase A OS=Ruminococcus albus OX=1264 GN=celA PE=1 SV=1
P16216	1.44e-89	50	416	55	405	Endoglucanase 1 OS=Ruminococcus albus OX=1264 GN=Eg I PE=1 SV=1
P28623	4.08e-89	58	406	38	369	Endoglucanase D OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=engD PE=1 SV=2
P28621	1.55e-88	58	406	37	371	Endoglucanase B OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=engB PE=3 SV=1
P54937	2.39e-88	64	409	40	378	Endoglucanase A OS=Clostridium longisporum OX=1523 GN=celA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000050	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001798_00259.