CAZyme Information: MGYG000001805_00266

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000001805_00266
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
713	MGYG000001805_4|CGC1	79810.48	6.8021

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001805	1812910	MAG	Denmark	Europe

• Gene Location: Start: 9997; End: 12138 Strand: +

Full Sequence      

MRQRSAKANYELKKSLLTILVSAAAAIPATAQHSGITDTRNSKFAVVNSTPIDAVKWTGG
FWGERFGVFSGTSVQSMWETWKSDRGHSYNNFLIAAGEKIDKRHGPAFHDGDMYKWLEAV
AAVYAVNKDPELDRIMDDVIRLIAKAQRPDGYLHTPVVIADRLKAANGGGKLAAEMAAAE
AKENEKIGTKLGTSKDGRFGNPLNFEAYNLGHLITAGIIHKRATGKTTLFDCAVKAADCL
YDFYMHPTADALEKGVICPSHYMAVAEIYRETGNKKYLDLANQLINIRDSVKNGTDDNQD
RIPFRQQYNAMGHSVRANYLYSGVADLYLEGGEAQLKKNLDAIWDDIVYRKMYINGACGP
LYDGTSPDGTEYNPDLIQKVHQSYGRPYQLPNSTAHNETCANIGNMLLNWRLFQATADAK
YTDLVENCFYNSILCGISLDGKKYFYTNPLRKSDDLPYKLRWPHERVEYISCFCCPPNTL
RTLCEAQDYAYSVGKGEIYVNLYGENTLTTKLDGIGEITIEQKTDYPWNGNIALTVAKLK
GKKDFTLKLRIPAWCDNATIKVNGETVNTDIKSQSYASVTRQWKKGDMVTIDMPMETKLM
EANPLVEECRGQVAVQRGPVIYCLESNDLNGVDIDNIAIPVDAKFTPVETTIEGSRMIAL
ETEAVNRSEASWENTLYREVSNNKNTVKIRLIPYYAWGNRGKSEMTIWIPAEF

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	55	623	3.8e-181	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	54	623	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	2.17e-133	59	712	20	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCD40914.1	7.28e-318	19	710	8	692
QUT70969.1	3.58e-316	12	711	1	696
QUT38421.1	7.69e-316	12	710	1	695
BCA50085.1	2.20e-315	12	710	1	695
QUT29106.1	3.12e-315	12	710	1	695

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQO_A	1.62e-316	12	710	1	695	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
6EX6_A	2.30e-120	47	712	7	626	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	1.49e-117	61	709	22	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	5.28e-114	61	709	22	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
7EXU_A	8.76e-81	85	707	50	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.02e-79	85	707	50	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.004831	0.993505	0.000643	0.000450	0.000279	0.000234

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001805_00266.