CAZyme Information: MGYG000001810_01863

Basic Information

• Species: Mediterraneibacter sp002314255
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp002314255
• CAZyme ID: MGYG000001810_01863
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2413	MGYG000001810_24|CGC1	266250.34	3.8834

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001810	2943094	MAG	Denmark	Europe

• Gene Location: Start: 17879; End: 25120 Strand: +

Full Sequence      

MKKVKKRVAGFLLAICMILSCVPVPAQAAASDNLALNRPASASDVESGTSFTADKAVDGS
SSTRWATNRDTNAVKTPRWLQIDFESTVTFDTVDISWEQANILSYEIQVSDDAGEDSWTT
VYSRESQPTMTEESISLSSPASGRHMRIYVSDFGGDWASVSIYEVGVYYLAGGEGPSDPD
GNYTIYPIPQKVTDEDGTVDLTDTVNVVAEDGIDTVTLDRADEVLTENGLTPQFDSEPSD
EYTNLYIGVDGSGGAADSLENIPREVFEEGENKYDMHVVKVFEDGDIVILGKDTDAAFYG
LATLEQMLDQTEDGQLKISTFEDYAFQKYRGCVEGYYGYPWSVEGTLSWFDFAKRYKMNT
FLYGPKNDPYHLGQWDEEYPTEVTEEEAELGLRTQDEMRQYAEKAAECNVDFVWVAHPAM
KKPIDFTNEETVDEGVARLMTKFEHMYDLGVRQFGIFVDDIDMSTAAATCDMQIYMLNQA
QEKLYDTYNTEGTAAEDCVKPLFFTPAWYTTGSGGASTYMPRFRALHEDIEICFTGNNVF
SNISNSSAATFKNWIGREPVMWWNYPVNDNVDNVYYTNPIDHFYSLDSNPSNLKGVISNP
MNYSESSKVAFFGLADYTWNPQAFDADENWEHCFDAILPDDPEMAQALKVVYGSMNYDYE
PADLTRLYAQYGSGNDAAAAELMDKMYEIVDSIEMLETLKDSDDPVKRLIVDEAQATINK
LYDMAAAIGGAMAAVSSDDPLVQTHGYYLANEAFERFSIERNPRYQIIALEGSGEDYYYS
TWYANPSDNRMKSFVTTAMDAISDFDVSALDTSAADIRSVVISPSDGVEVRQGESLQFTA
AVEAGQENTDDVIWTVDGNTGDGTEINWNGILTLDTNELSPTVTVKAVSAYDDTVFDTVE
VTVADRIYVDPTIPVNQAYGAKVLGASGNPGVGGGPENLFDESEDGSKWCPGDNTRYNHW
AAFDLGAEKTIYTWETVHAGVEHELDISSDFSLQILADPNATEEQLSDSSYLGNAANWIT
VAEYRDNTENITDYEFDEPVTARYFRLYVADGCQDGVPYAATRIYELRLFGVDTATVART
HSLTIDPSITGGTVSTDASNYEEGAKVNIYIEPEEGYQLAEGSLKYNDTVIEGTSFLMPA
EDVVLTAEFTDGSEEPGPDPDPDPEPGWDDENALAALVNPVVQKYELAKKSGTWTMTADT
RFAVASDQENIENERLAEVVKLINSEFAEKGLVSSSPLAMVYANEEDTQPADILITLDKD
GAVCEESDSEEAYTIEISADGVKITGASENAVLYALRTVQNYMTANKGLPYGKITDYPDV
AERRLHVDCGRKYFTKDWFIRQIREMSYMKMNTLEMHFSENLGFGIECKTDPSIVSEQYL
THEEVREILAEAAKYGIKVIPSFDSPGHVDQILRAHPEYGQVNINGDHYASGLDVTNPEA
VAYIRSLYDEYMELFEGCTDFHIGGDEYMEFDRAPFTTQYKPVLNAYAAETLGEGATWKD
VMANYINELAEYVYSKGFKPRVFNDGLYYGETDWEGPQQIVMHDYIGIDYWSKMTWNPAI
AGMQTFIDKGHEDIYNFNSTFFYYVLRNDMPTDGREQHSFDYIDQDRRIFEEWTPGQFAS
YTAPDDASYIAGAAMGIWCDNPDLVTEDVVTEDIADEMRSLATKSWNVSSSDNMDFEQFK
ANYEVLGHVAGYEKGSVLPDAGEFQNAESLGKVTLCYVSDTGSKLREDVVKYGNAGDAYT
FEAEEIYGYRLISEGTVSGIYTSEGETYTFTYTLDTDKSELEQEIADGLDAAYYIPETFE
AYRQAYAAAEEAAGRDDIEQTEIDEVLGQLRDAKAQAVLLVNYPLYVEVTYPLSDIGYAS
GYSEYEAAVEEGRQILYGQGDDPAAVTAAFDKIRQAADALEMPGVGMPEISADDGYYTTY
SYDKMLDGSEATKCWFNKDQTAGRQFMFTFPVTVSLSGIRILQPSDVGADYIEGAEVQIS
ADGESWQTVGTIGPDDRDTELSFEAVPAKYVRAVLTETKKNWYQIAEVSFTYEEIPEDTT
LRELIREAEDLDISGKDTELVNSMIAALIEAQKLYAQDSADTAEAQAVLEAAVNALKETQ
EPAEELSTAVLEYAIELAEGIDIDSDVVDAVKENFENALQNAKDILERVQAGDAGVKQSD
VDSAWQNLIRAIQYLEFKQADKTDLEKVIALAEEMNSNLDAYLDAGKEAFTTALAAAKEV
YEDVNAMDQEEVNTAWQNLLDAMANLMLRPDKGLLEDLIAQAESLNEADYDAQSFAVMRA
ALAAAKETAADETALQDEVDASAAALEDAIAKLTASGSGDDQQSGGAQQSGDDQQAGTGG
SQSGNGSQSQGQTAGSSDNKSTASASGAAAASNASAVQKSVKSGDNGNYILWCAAMLLAA
GTAAAAYRKKRQK

Enzyme Prediction     

No EC number prediction in MGYG000001810_01863.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	330	650	2.4e-74	0.9864406779661017
GH20	1321	1667	6.6e-61	0.9525222551928784
CBM32	42	165	2e-21	0.9274193548387096

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	4.84e-103	330	650	1	292	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
cd06564	GH20_DspB_LnbB-like	6.83e-61	1322	1666	2	324	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
cd02742	GH20_hexosaminidase	1.24e-33	1323	1666	2	302	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	3.91e-32	1323	1662	4	337	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
COG3525	Chb	1.59e-30	1270	1641	205	590	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCL57158.1	0.0	1171	2323	52	1204
ASK64148.1	4.78e-224	1174	1709	34	577
APC47599.1	8.72e-217	1174	1713	34	581
AYV34923.1	2.84e-172	1178	1706	41	569
BCL57159.1	8.91e-167	1180	2246	48	1076

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	4.38e-75	183	771	34	587	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV4_A	2.45e-69	183	634	32	473	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PV5_A	7.78e-56	177	650	34	485	Structureof CpGH84B [Clostridium perfringens ATCC 13124]
5OXD_A	6.58e-51	172	650	1	449	Complexof a C. perfringens O-GlcNAcase with a fragment hit [Clostridium perfringens]
2CBI_A	9.52e-51	175	650	2	447	Structureof the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBI_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBJ_A Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2CBJ_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2V5C_A Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2V5C_B Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2VUR_A Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2VUR_B Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2X0Y_A Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens],2X0Y_B Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	1.03e-65	183	634	39	480	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q8XL08	3.24e-48	175	650	32	477	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q89ZI2	3.49e-48	286	639	110	433	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q0TR53	4.29e-48	175	650	32	477	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
B2UPR7	9.66e-23	1251	1689	167	590	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000543	0.635178	0.363486	0.000314	0.000250	0.000208

TMHMM  Annotations     

start	end
7	29
2388	2407