CAZyme Information: MGYG000001831_03016

Basic Information

• Species: TF01-11 sp000436755
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; TF01-11; TF01-11 sp000436755
• CAZyme ID: MGYG000001831_03016
• CAZy Family: GT35
• CAZyme Description: Maltodextrin phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
747		85931.86	5.6053

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001831	3856267	MAG	Denmark	Europe

• Gene Location: Start: 1216; End: 3459 Strand: +

Full Sequence      

MYENEIENIVSRSSDEEIYYGLLEFTKNMAQQRGKIEGKKKLYYISAEFLIGKLLSNNLI
NLGLYDQVAKALEKYGKSMAQIEEIEPEPSLGNGGLGRLASCFLDSIATLGLNGDGVGIN
YHLGLFKQEFDKNLQKEVPNPWIKDKSWLRRTDKSYEIIFGGLRVNSVMYEIDVTGYDNK
CNTLKLFDIDSVDDSIVQGDSIYFNKEDIRKNLTLFLYPDDSDYQGQLLRIYQQYFMVSN
AAQLIIDEAVAKGSNLYDLADYAVVQINDTHPTMVIPELIRLLVKKGIDMDKAIDIVSHM
CAYTNHTILAEALEKWPIWFLEKVVPQLMPIIKMLDVKVKEKYNNPELAIIDDKQQVHMA
RIDMHYGFSVNGVAALHTEILKNSELKPFYDIYPNKFNNKTNGITFRRWLLHCNHELTDF
ISSKIGDGFKKDANELEKLLQYKDDVAVLNAITNIKMTKKKEFKEYLLKKENIEIDENSI
IDVQVKRLHEYKRQQMNALYIIHKYMDIKAGNKPKRPITFVFGAKAAPAYTIAKDIIHLI
LCLSKVIENDPEVSPYLKVVMLENYNVTYAEKIIPAADISEQISLASKEASGTSNMKFML
NGAVTLGTEDGANVEIHELVGDENIYIFGEKSDKVIEHYAKADYSAEKIYDSDTEIAKLV
DFIIDKKMFAVGNKKSLIRLYMEIVTKDWFMTLLDIKDYIRVKEQVFNDYEDRQAWSRKM
IKNIAMAGFFSSDRTIKQYNNDIWHLD

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	71	746	6.7e-225	0.9970326409495549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	40	747	70	811	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	31	747	53	749	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	44	744	63	794	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	13	746	16	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	13	746	19	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEN95921.1	0.0	10	746	15	750
QUF80147.1	0.0	10	747	15	751
SOB70959.1	0.0	10	747	15	751
VCV23296.1	0.0	10	746	37	772
CBL08110.1	0.0	10	746	28	763

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4L22_A	0.0	14	745	24	756	Crystalstructure of putative glycogen phosphorylase from Streptococcus mutans [Streptococcus mutans UA159]
2C4M_A	1.98e-172	43	747	63	789	Starchphosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_B Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_C Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_D Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae]
1Z8D_A	1.13e-164	31	747	72	829	ChainA, Glycogen phosphorylase, muscle form [Homo sapiens]
2FFR_A	1.37e-164	31	747	60	817	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
5OX0_A	1.63e-164	31	747	72	829	GlycogenPhosphorylase in complex with CK898 [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P29849	0.0	3	747	9	752	Maltodextrin phosphorylase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=malP PE=3 SV=2
P39123	4.02e-169	32	746	53	794	Glycogen phosphorylase OS=Bacillus subtilis (strain 168) OX=224308 GN=glgP PE=2 SV=1
Q9WUB3	7.85e-165	31	747	72	829	Glycogen phosphorylase, muscle form OS=Mus musculus OX=10090 GN=Pygm PE=1 SV=3
P11217	7.85e-165	31	747	72	829	Glycogen phosphorylase, muscle form OS=Homo sapiens OX=9606 GN=PYGM PE=1 SV=6
P0AC86	1.01e-164	40	747	70	811	Glycogen phosphorylase OS=Escherichia coli (strain K12) OX=83333 GN=glgP PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000073	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001831_03016.