CAZyme Information: MGYG000001850_00422

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; QALW01; UMGS1322
• CAZyme ID: MGYG000001850_00422
• CAZy Family: GT2
• CAZyme Description: Ubiquinone biosynthesis O-methyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
860		98769.3	6.3481

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001850	2350438	MAG	Denmark	Europe

• Gene Location: Start: 30159; End: 32741 Strand: +

Full Sequence      

MNSVYDESYYTRYRLSEEQTVNYSGCLHLQKFFRRIAETIVEKYHPKTVLDAGCALGMLV
KALRDLGVEAYGIDISEYAITHTDPSVSSYCRVCSLTEPLPGEFPTHFDMITCVEVLEHL
TEADGKKAIANLCRYTDMVLFSSTSKKDEDPTHLNVRTSDFWASAFAEVGFYNQTDRYPS
FLSADAYLFVRNASYLPAVERYERTINCKKAEIGAVSQQAEAQRIMLQENISLLKETLAL
QKELAENVKRENNTLNIQLQHTLAAYQQISRSQFWRITYPLRFIADQIKKLLKKIRSLLK
KNEKISQEKCYSISKSRRKEESKKVFDRPVKFSILVPLYNTPKSFLCEMIESVQKQTYAK
WELCLADGSDEQHRDVEKICMSAAKSDSRICYKKLKENKGISANTNECIQMATGDYFALL
DHDDILHPSALFSCMEVICKENADFIYTDEATFVNNSLDDIVLRHHKPDFSPDTLRSYNY
ICHLSVFSRDLLEKVGPFNSEYDGSQDYDLILRLTEQAKKIVHIPELLYFWRGHADSTAA
SVAAKPYIVEAAHKALAAHLERVGLKGEVKDGAVPSVYKIEYELNGTPLVSILIPNKDHI
ADLEKCLQSIYEKTTYQNFEIIVIENNSEHPTTFQYYETLKKYPRLQVVKWTREFNYSAI
NNFGAQYASGEYLLLLNNDIEIISENWIQEMLMFAQRKDVGAVGAKLYYSDDTIQHAGVI
LGICGIAGHSHKYFHRSNTGYMTRASIIQNLSACTAACLLLRRDVFDEVGGLDEKFQVAF
NDVDLCMKIRRAGYLIVFTPYAEMYHYESKSRGAEVTPQQQERFRSEINRFQEKWGKELE
AGDPYYNPHLSLETEQFDIK

Enzyme Prediction     

No EC number prediction in MGYG000001850_00422.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	333	495	6.5e-24	0.9882352941176471
GT2	591	770	3.9e-22	0.9941176470588236

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04184	GT2_RfbC_Mx_like	1.77e-82	331	532	2	201	Myxococcus xanthus RfbC like proteins are required for O-antigen biosynthesis. The rfbC gene encodes a predicted protein of 1,276 amino acids, which is required for O-antigen biosynthesis in Myxococcus xanthus. It is a subfamily of Glycosyltransferase Family GT2, which includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds.
cd04186	GT_2_like_c	5.14e-47	592	808	1	166	Subfamily of Glycosyltransferase Family GT2 of unknown function. GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.
COG1216	GT2	7.17e-32	586	813	1	226	Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism].
cd06433	GT_2_WfgS_like	8.28e-26	333	538	1	200	WfgS and WfeV are involved in O-antigen biosynthesis. Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.
pfam00535	Glycos_transf_2	2.06e-22	333	492	1	164	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAB1243278.1	6.57e-286	2	860	6	1033
QUC66692.1	3.06e-258	199	860	252	946
QMW89744.1	1.01e-235	313	860	66	615
BBK78193.1	1.01e-235	313	860	66	615
QUO22278.1	5.18e-226	309	860	76	629

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3BCV_A	1.47e-07	331	448	6	119	Crystalstructure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3BCV_B Crystal structure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5HEA_A	3.21e-06	589	691	6	105	CgTstructure in hexamer [Streptococcus parasanguinis FW213],5HEA_B CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEA_C CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEC_A CgT structure in dimer [Streptococcus parasanguinis FW213],5HEC_B CgT structure in dimer [Streptococcus parasanguinis FW213]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P55465	1.35e-138	333	857	369	895	Uncharacterized protein y4gI OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a03550 PE=4 SV=1
Q50864	1.72e-92	328	852	318	833	O-antigen biosynthesis protein RfbC OS=Myxococcus xanthus OX=34 GN=rfbC PE=4 SV=1
Q58457	1.45e-11	327	532	6	212	Uncharacterized glycosyltransferase MJ1057 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1057 PE=3 SV=2
B5L3F2	2.16e-11	333	516	7	181	UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-glucosyltransferase WfgD OS=Escherichia coli OX=562 GN=wfgD PE=1 SV=1
P39614	1.25e-09	330	545	1	214	Uncharacterized glycosyltransferase YwdF OS=Bacillus subtilis (strain 168) OX=224308 GN=ywdF PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001850_00422.