CAZyme Information: MGYG000001855_01458

Basic Information

• Species: CAG-632 sp000431515
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-632; CAG-632 sp000431515
• CAZyme ID: MGYG000001855_01458
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
734	MGYG000001855_5|CGC1	85607.21	5.0896

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001855	2470907	MAG	Denmark	Europe

• Gene Location: Start: 119198; End: 121402 Strand: +

Full Sequence      

MKIKKDVFAINWMEVDALVKARHDDPHHILGMHECIDDIYVNVFQPTAKQITVINEKTKE
EYALEAKRVSGFFSVKIPKCKRFDYSIRMTKEDGTEYTYIDPYMFESVIDSIDISLFNEG
MHYEIYEKLGAHPMTVDGVAGVLFAVWAPNAERVSVVADFNHWDGRIHQMRRLPYSGIFE
LFIPGVGENEIYKYEVRSRNGELKMKSDPYGNASELRPCNASIVTDLRKYEWKDDAWMQN
RDKVEASKAPMSIYEVYLGAWKRPTDGREYYNYRELAVKLAEYMKDMGYTHIELMPVMEH
PYDPSWGYQVTGYYAPTARYGSPEDFMYFVDYMHEQGISVIIDWVPAHFPKDENGLGRFD
GSCVYEHEDPRRGEHPHWGTYIFDYSKPEVKNFLIANALFWADKYHVDGIRMDAVASMLY
LDYGRDFGNWLPNMYGGNENLDAIDMLKQLNTVMKERYPSCIMIAEESTAWPMVSHPVAD
GGLGFDFKWNMGWMNDFLEYMKLDPLYRKYHHNELTFSMVYAYSEHFILVLSHDEVVHEK
GSMIAKMPGGYEDKFSNLRLAYGFMMTHPGKKLLFMGQEFAQFKEFNESDELDWGIFEFD
AHVCIRDYCRALNHLYQQEPALYALDDDPEGFEWINSIASNESLLVFARKTEKKEDTLVI
VCNFTPVEHVNYNIGVPYPGKYKEIFTSEEEQFGGEGKKNKTLKQSKKVVCDERENSISI
YIAPLSISVFKFYK

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	282	582	3.3e-157	0.9966777408637874
CBM48	128	214	2.9e-18	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	113	732	11	627	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	23	731	516	1221	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	104	731	1	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14706	PRK14706	0.0	129	732	27	621	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	13	731	4	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM00753.1	0.0	1	731	1	730
QWT54329.1	0.0	13	731	1	718
QBE99030.1	0.0	10	734	9	734
CBK76766.1	0.0	10	730	9	740
QIB58408.1	0.0	10	734	9	734

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQZ_A	5.60e-257	14	731	29	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR5_A	1.12e-256	14	731	29	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	1.12e-256	14	731	29	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A	4.52e-256	14	731	29	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	6.41e-256	14	731	29	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8DLB8	3.62e-276	14	731	7	749	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
P52981	3.53e-262	8	731	3	751	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1
Q2JT08	2.31e-260	8	732	3	753	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
Q608L5	6.67e-260	14	726	13	719	1,4-alpha-glucan branching enzyme GlgB OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=glgB PE=3 SV=1
Q1AZ86	1.02e-259	15	726	7	713	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000060	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001855_01458.