CAZyme Information: MGYG000001860_01863

Basic Information

• Species: UMGS172 sp900539855
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; UMGS172; UMGS172 sp900539855
• CAZyme ID: MGYG000001860_01863
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
618		69417.58	5.2421

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001860	1972754	MAG	Denmark	Europe

• Gene Location: Start: 242; End: 2098 Strand: -

Full Sequence      

MRQIPFDNVKLGGFWGARQKLNREVTINSVYDRFSDTGRFDAFKCNWQNGDPNKPHFFWD
SDVAKWIESVAYITADKKDERLEKIVDGVVDLIEENADKDGYYNSFYLTLEPYAKFKLRD
RHELYCLGHLIEAAVAYDKATGKDKLLRIVCRYADHVKKIFIDEHKVPFYTPGHEEIELA
LVKLYRHTKDKKYLQMATWFIDERGKHPTEDTVLFGTAKPAYSQDQCPVREMSTAEGHAV
RMMYLLCGMADTAVENDDNELFDACRRVFDNIINRRMYITGGVGSSSDGEAFTVDYDLPN
LLAYTETCAAISMVFFATRMAAAEPDSKYADTAERALYNGFLSGVSLDGKKFFYSNPLEI
YSPFKTRNASLTSSSIKLPASQRVEVFECSCCPPNVTRMIASLGGILYSAGTDTLFIHHY
AKSSARIELDGNAVSIDQDTLYPADGNVNIKITGACGKMVAVRIPGWCQQYKIETSDKED
ISDIRNGYAYIDVTSDEFTITAEFSMPVVLIESSPHVRENAGKVAVMRGPLVYCAEAVDN
GDDLFALSLDSSLEGAAVESADGWLNTLVLKGRRKTASGGTLYMPLSTDTVPVSVRLIPY
YAFANRGESNMQVWFRKY

Enzyme Prediction     

No EC number prediction in MGYG000001860_01863.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	12	534	1.1e-184	0.9904580152671756

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	5.70e-171	2	616	10	586	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	2.57e-149	8	534	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG4225	YesR	3.48e-05	114	203	26	114	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUS96918.1	6.44e-193	4	615	12	625
QHQ63226.1	1.17e-176	2	615	7	622
AZN43118.1	1.44e-162	13	617	23	656
ADH61394.1	1.35e-161	1	617	9	647
AZO96465.1	3.56e-160	1	617	8	651

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	2.85e-156	58	614	74	644	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.57e-151	58	614	74	644	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	1.35e-118	4	614	10	622	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
3WRE_A	5.05e-114	58	617	73	658	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	6.79e-114	58	617	73	658	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	2.77e-113	58	617	73	658	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001860_01863.