CAZyme Information: MGYG000001862_01575

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Anaerotignaceae; Anaerotignum
• CAZyme ID: MGYG000001862_01575
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
753		87673.39	6.3125

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001862	2256861	MAG	Denmark	Europe

• Gene Location: Start: 2238; End: 4499 Strand: +

Full Sequence      

MITIKHLNELFQIINGEHADPHTVLGMHEVEEDGKKCVVVRAFIPGAQTITVVDANTRRL
KYPMERIHEDGFFEVIIPDREEWFRYRLECTDYQGNSWHTYDPYAFSPTISEYDRYLFGE
GNHYEIYEKLGAHLITFEGAKGVSFAVWAPNAKSVSVIGNFNGWDTRRHPMRLLGESGIW
ELFIPGLAAMDQYKFHVVQSTGKQVDKCDPYAFYAQLRPETASVIYPIGRYKWKDRKWMT
ARKKANVYTAPMNIYEVHLGSWMRVPEEGNRFLTYTEMAEKLVPYVKEMGFSHIELLPVE
EHPFDGSWGYQVTGYYAPTSRYGTPAMFKEFIDTCHQNGIGVILDWVPAHFPKDEFGLGR
FDGTALYEHQDPRRGEHLQWGTYIFNYGRKEVSNFLIANALFWLDEYHIDGLRVDAVASM
LYLDFCKNDGEWAPNEYGGRENLEAVEFIKHMNSIVSKRLPGTMMIAEESTSWEGVTKSA
DDNGLGFTMKWNMGWMNDFLFYIKKDPIYRKYHHNNLTFGMAYNYSENFVLVLSHDEVVH
EKSAMIGKMPGDLWQKYANLRLSYGFMYGHPGKKLLFMGGEFAQFNEWSEARSLDWHLLQ
YKDHQQMQLYMKDLNHFYLSESPFWQQDYDPHGFTWIECDDAESSVVSFIRRSQDKELYF
ICNFTPETYEDFRIGVPFAGTYKEVFNSDAEKYGGSGVVNKKAAVSEKMPWNHYQNSIRV
KVPPLGMVVLERKVPEEAKAEKKTTKKTEKKKA

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	284	584	1.6e-159	0.9966777408637874
CBM48	129	214	3.6e-20	0.8947368421052632

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	107	733	4	628	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	130	731	27	620	glycogen branching enzyme; Provisional
PRK14705	PRK14705	0.0	10	726	508	1216	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	9	733	5	724	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	0.0	105	732	1	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMJ39890.1	0.0	4	742	14	749
ANV77640.1	0.0	1	730	1	729
ALX09866.1	0.0	1	730	1	729
ABN53393.1	0.0	1	730	1	729
ADU75834.1	0.0	1	730	1	729

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR5_A	9.69e-286	1	736	21	780	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A	1.95e-285	1	736	21	780	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQZ_A	7.86e-285	1	736	21	780	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	7.86e-285	1	736	21	780	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A	1.11e-284	1	736	21	780	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8DLB8	9.50e-299	9	730	7	748	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
P52981	9.60e-290	8	730	8	750	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1
Q8YYX9	1.03e-287	12	740	13	761	1,4-alpha-glucan branching enzyme GlgB OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=glgB PE=3 SV=1
Q7NL20	4.57e-286	7	733	7	729	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
Q3M473	5.49e-285	12	740	13	761	1,4-alpha-glucan branching enzyme GlgB OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001862_01575.