CAZyme Information: MGYG000001867_00648

Basic Information

• Species: CAG-873 sp900555715
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-873; CAG-873 sp900555715
• CAZyme ID: MGYG000001867_00648
• CAZy Family: GH65
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
676		75553.48	4.9972

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001867	2424881	MAG	Denmark	Europe

• Gene Location: Start: 5800; End: 7830 Strand: +

Full Sequence      

MYIKNLIAATVMLLPAVTGWASVDKPDDWRIVAEDFNNYNGVTLANGTIGIVTSDKLLKI
DDIVLNNVYMSRRPGDVSRIARGPHFLNLDIDIDGRAVTDSTICHKRQVLDMKEAKMTTT
FDADGAEISYEVMAMRNLPYMGLVRVKVKAERDVTLDVKNQTSFAAEYVDCSAKFKNHRD
GNHLMPAMTSMATSYDGSIEVASATAFLFPSEEHPEIVSEDISDGYPIMKFTAQVKKGEI
FEFSLMGAVANSVEFPHLDADVERMAVYALRSDLDALVDGHKKEWERLWESDIIIEGDDE
SQRDVRLALYHLYSFGGEGTRNSIAPMGLSTVSGYNGHVFWDTEMWMYPPLLMLNQDMAR
SCVDYRTDRLPQACRRARHYGFCGAMYPWESDRSGEEATPTWCLTGTFEHHITADIAIAF
WNYYRVTRDIDWLRAEGYEVMKNVADFWKSRATLNADGSYSIKNVVGANEYAANIDDNAF
TNGAAKRALEYAVKAANTIGLTPDPAWEKIADGIKFHHMPDGTTMEHSRYAGEIVKQADV
NLLAYPLEIVTDRDRILADVEYYAKKIDGNGPAMGNSILAVLYSQVGDADKAYEFFHKAF
MPNKRPPFGVLSESANSNNPYFCTGAGGLIQVVLCGFAGLRFTDNGIEQAYTSLPKEWKS
LTIKGVGPDKKTYVIR

Enzyme Prediction     

No EC number prediction in MGYG000001867_00648.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	306	644	2.3e-100	0.9946236559139785

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1554	ATH1	2.01e-100	74	661	72	718	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
pfam03632	Glyco_hydro_65m	5.10e-65	306	647	1	387	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.
PRK13807	PRK13807	5.54e-45	83	664	84	710	maltose phosphorylase; Provisional
pfam03636	Glyco_hydro_65N	7.11e-08	84	239	69	227	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWX45602.1	1.23e-238	4	676	2	678
SDS46993.1	1.50e-234	22	675	16	676
SDB50400.1	3.02e-234	10	673	4	674
AXT56849.1	2.05e-233	11	675	13	682
AUP80081.1	1.29e-229	22	675	16	672

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	1.94e-183	27	675	22	676	ChainA, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	7.59e-49	174	661	177	714	Crystalstructure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]
1H54_A	1.13e-32	74	670	75	718	ChainA, Maltose Phosphorylase [Levilactobacillus brevis],1H54_B Chain B, Maltose Phosphorylase [Levilactobacillus brevis]
4KTP_A	1.96e-29	177	666	171	714	ChainA, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTP_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]
4KTR_A	1.89e-28	177	666	171	714	ChainA, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_C Chain C, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_D Chain D, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_E Chain E, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_F Chain F, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_G Chain G, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_H Chain H, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q54KX5	1.96e-71	275	666	252	650	Protein-glucosylgalactosylhydroxylysine glucosidase OS=Dictyostelium discoideum OX=44689 GN=pgghg PE=3 SV=2
A0JMP0	8.65e-59	281	666	186	585	Protein-glucosylgalactosylhydroxylysine glucosidase OS=Danio rerio OX=7955 GN=pgghg PE=2 SV=1
Q32M88	3.63e-51	276	647	226	609	Protein-glucosylgalactosylhydroxylysine glucosidase OS=Homo sapiens OX=9606 GN=PGGHG PE=1 SV=2
Q8BP56	2.54e-50	281	647	230	608	Protein-glucosylgalactosylhydroxylysine glucosidase OS=Mus musculus OX=10090 GN=Pgghg PE=1 SV=1
Q8L163	2.14e-49	230	664	249	735	Kojibiose phosphorylase OS=Thermoanaerobacter brockii OX=29323 GN=kojP PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000544	0.998653	0.000249	0.000189	0.000182	0.000173

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001867_00648.