CAZyme Information: MGYG000001868_01298

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485
• CAZyme ID: MGYG000001868_01298
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
442		50038.25	9.1156

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001868	1969203	MAG	Denmark	Europe

• Gene Location: Start: 2777; End: 4105 Strand: -

Full Sequence      

MGFKLHKEKDGQRDRAGRTARIVKYMWLGFCGLGLAIAIFLLLIYHGVIGYMPSIEELED
PHDKLASQIYAMDGNQEKEIGRFFEGAGNRVNSSYDSVAPVLIDALVATEDVRFYEHSGI
DFRALGRTMVKTLMMGDKSQGGASTITQQLAKQLYSTPSPSLMKRALQKPIEWMIAIKLE
RFYTKEEILTMYLNRFDFLNNAVGIKTASNVYFGKDPDKLQTQEAAMLVGMLKNPSYYNP
LRYPDRVRDRRNVVLDQMVKAGKLSAVEAEGYKSMDLGIDYHRVDHKEGLAPYFREEIRR
MMTARKPKRPNPGDYGSKLAFTIALSNYNTDSTNWATDPLYGWIEKNPKPDGTTYDLYKD
GLNIYTTLDLKMQEYAEDAVMEYVGGVLQPAFSAEKRSNKYGPYSTVASELPAGGAKQLV
DNAIKKTERYRIMKEAGHSEAR

Enzyme Prediction     

No EC number prediction in MGYG000001868_01298.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	78	259	1.5e-60	0.9830508474576272

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	1.43e-77	21	421	3	340	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG0744	MrcB	9.41e-76	12	392	3	330	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	2.20e-73	89	380	1	240	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam00912	Transgly	3.63e-72	78	259	3	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
PRK11636	mrcA	5.16e-49	21	384	2	307	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASB36812.1	2.05e-164	19	441	8	419
ANU62694.1	2.05e-164	19	441	8	419
QCD41112.1	1.16e-163	11	439	32	450
QQR10353.1	4.33e-163	26	441	1	405
QCP72948.1	2.08e-161	5	439	9	438

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3NB6_A	2.50e-33	86	270	16	195	Crystalstructure of Aquifex aeolicus peptidoglycan glycosyltransferase in complex with Methylphosphoryl Neryl Moenomycin [Aquifex aeolicus]
2OQO_A	6.71e-33	86	270	16	195	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]
5U2G_A	8.74e-33	52	348	7	318	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3UDF_A	2.44e-32	68	382	22	326	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	2.96e-31	55	305	9	248	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P02918	5.30e-40	44	384	25	307	Penicillin-binding protein 1A OS=Escherichia coli (strain K12) OX=83333 GN=mrcA PE=1 SV=1
P38050	3.65e-35	89	325	66	293	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q9KNU5	5.35e-34	52	413	33	353	Penicillin-binding protein 1A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcA PE=3 SV=2
P39793	1.06e-33	53	378	64	337	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
O66874	1.07e-33	23	382	1	300	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000018	0.000007	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
27	49