CAZyme Information: MGYG000001876_02105

Basic Information

• Species: Acetatifactor sp900760705
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Acetatifactor; Acetatifactor sp900760705
• CAZyme ID: MGYG000001876_02105
• CAZy Family: GH2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
866		100634.84	5.0067

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001876	3172346	MAG	Denmark	Europe

• Gene Location: Start: 6721; End: 9321 Strand: -

Full Sequence      

MEKVRIDSGWRMRSLEEKEFVTAKVPGSVYQAYLDNGKMEDPYYRDNELKALKLMEQDFE
YVTEFEVPENVINREEVILRFAGIDTLGEIWLNGHFLGKTDNMHRIWEYRVKALLQEEKK
NELRVVLHSPVKYLRERYEKDPKIMIVDEGIRGASYLRKAHCMFGWDWGPRLPDAGIFRE
VSLLGVDSARFDNVYVIQKHEAGKVELSVSAEAEVLGEERLYRYSADGTGAGEVYRYVEE
HGKDGENPYRLDHPASLHREKTDTEALQEKGYEVSVCVYDPDGKCVAQGDALERLKIEEP
RLWWPSGYGEQPLYTVKAVLKKDGAVLDVWEKRIGLRTMTMAREEDEWGECFCHEVNGVK
IFAMGADYIPEDNILPRVNKERTRHLLEQAKAAHFNSIRVWGGGYYPSDDFYDCCDELGL
VVWQDFMFACAVYDLTPEFEANIRAEFADNVKRLRHHASLGLWCGNNEMEQFLVGDGVKY
WCKKQSQIADYFTMYHHVIPGMLRVLDPQTFYWPSSPSSGGRADDANDETCGDVHYWAVW
HGNKPITEFRKFHFRYLSEFGFQSFPGIETVKTFTEPEDRNIFSYVMETHQRNSSANGKI
MNYMEQTFKYPTNFETLLYASQLLQAEAMRYGVEHFRRIRGCCMGTVIWQLNDCWPVASW
AMIDYEGRWKAVQYYAKRFFAPLMISCAEEGVLSQNPNVNAEPYEVKKSIHLCVCNETME
NRIVQVRWQLRNAEGTVLKEEGEEISVQALSSQWLEKEEMPQARLREDYVYYELKENGQV
ISSGTVLFGVPKYFNFRDPKLKAERISADEIQVTSEAYARSVEILNGDDTMLLEDNYFDM
NPGTRILRVVKGEPKDLKVRSVYDIR

Enzyme Prediction     

No EC number prediction in MGYG000001876_02105.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	777	3.9e-102	0.7194148936170213

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.45e-97	1	714	9	665	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17786	Mannosidase_ig	3.68e-12	710	793	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam00703	Glyco_hydro_2	3.55e-11	272	337	36	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	1.36e-09	61	468	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.40e-09	58	338	66	277	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL35923.1	0.0	6	862	6	820
QBE97789.1	0.0	1	866	15	837
AEF82182.1	0.0	5	866	5	816
QIB54534.1	0.0	1	866	1	823
QMW79049.1	0.0	1	866	1	823

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	2.19e-149	1	866	3	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	2.25e-149	1	866	3	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYI_A	1.60e-148	1	866	1	846	Crystalstructure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYI_B Crystal structure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYG_A	1.64e-148	1	866	3	848	Crystalstructure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYG_B Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	3.78e-141	2	864	7	836	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0CCA0	3.09e-114	8	690	11	661	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
B8NW36	6.41e-113	6	690	9	663	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1
Q2TXB7	8.95e-113	6	690	9	663	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
A1D911	4.86e-112	1	690	4	663	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
Q4WAH4	1.37e-110	1	690	4	663	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001876_02105.