CAZyme Information: MGYG000001893_01460

Basic Information

• Species: CAG-145 sp900542565
• Lineage: Bacteria; Firmicutes_A; Clostridia; Peptostreptococcales; Anaerovoracaceae; CAG-145; CAG-145 sp900542565
• CAZyme ID: MGYG000001893_01460
• CAZy Family: GH18
• CAZyme Description: Spore germination protein YaaH

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
309	MGYG000001893_19|CGC1	34723.61	4.6255

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001893	2137652	MAG	Denmark	Europe

• Gene Location: Start: 42412; End: 43341 Strand: +

Full Sequence      

MIVNGYAYPTTVHTALNWWLPRLTWLSVFSYGITEGGRLTVLDDEELIRAANNAGVRPLM
VLAPIDDKGQFAPDAFVAVLNDPAARSSLLYEIQANIRTKGMGGINFDVEYLPAEYASAY
AEFIKDMQKMLSPLGYLTTVALAPKISSDQKGVLYEGHDYKKLGEAADYCIIMTYEWGYT
YGEPLPVSPITNVRHVLEYGMSQITPSKILMGLNNYGYDWTLPFIKGESRAETLGNYQAA
ARAEYYGVPVLWDETAMAPYFNYTDPYGREHIVWFENERSWQARIAMADEMGLAGVSIWT
IKKIFYGGI

Enzyme Prediction     

No EC number prediction in MGYG000001893_01460.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	39	301	2.8e-38	0.7736486486486487

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02874	GH18_CFLE_spore_hydrolase	1.16e-104	3	301	4	302	Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
COG3858	YaaH	1.58e-82	1	301	102	407	Spore germination protein YaaH [Cell cycle control, cell division, chromosome partitioning].
smart00636	Glyco_18	1.59e-29	23	301	26	331	Glyco_18 domain.
cd06549	GH18_trifunctional	8.32e-29	16	299	24	288	GH18 domain of an uncharacterized family of bacterial proteins, which share a common three-domain architecture: an N-terminal glycosyl hydrolase family 18 (GH18) domain, a glycosyl transferase family 2 domain, and a C-terminal polysaccharide deacetylase domain.
cd02876	GH18_SI-CLP	5.24e-25	76	301	83	307	Stabilin-1 interacting chitinase-like protein (SI-CLP) is a eukaryotic chitinase-like protein of unknown function that interacts with the endocytic/sorting transmembrane receptor stabilin-1 and is secreted from the lysosome. SI-CLP has a glycosyl hydrolase family 18 (GH18) domain but lacks a chitin-binding domain. The catalytic amino acids of the GH18 domain are not conserved in SI-CLP, similar to the chitolectins YKL-39, YKL-40, and YM1/2. Human SI-CLP is sorted to late endosomes and secretory lysosomes in alternatively activated macrophages.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOX63466.1	2.79e-112	1	308	14	321
QAT42169.1	1.12e-104	1	303	4	318
QIB68537.1	2.40e-101	1	303	4	318
QHI71782.1	1.11e-99	1	303	4	318
BCJ94528.1	3.28e-99	1	305	107	411

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3J_A	8.63e-66	21	305	131	414	Crystalstructure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_B Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_C Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876]
4S3K_A	4.39e-63	23	305	135	416	ChainA, Spore germination protein YaaH [Priestia megaterium QM B1551]
3CZ8_A	1.62e-49	9	301	19	308	ChainA, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168],3CZ8_B Chain B, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168]
4WIW_A	3.93e-26	34	299	47	327	ChainA, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_B Chain B, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_C Chain C, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_D Chain D, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_E Chain E, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_F Chain F, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2]
4Q6T_A	1.60e-21	30	299	37	322	Thecrystal structure of a class V chitininase from Pseudomonas fluorescens Pf-5 [Pseudomonas protegens Pf-5]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0DPJ9	1.57e-65	21	305	130	413	Cortical fragment-lytic enzyme OS=Bacillus anthracis OX=1392 GN=sleL PE=2 SV=1
Q9K3E4	1.22e-64	21	305	130	413	Cortical fragment-lytic enzyme OS=Bacillus cereus OX=1396 GN=sleL PE=1 SV=1
P37531	6.95e-64	21	305	129	412	Cortical fragment-lytic enzyme OS=Bacillus subtilis (strain 168) OX=224308 GN=sleL PE=1 SV=2
O05495	2.44e-48	9	308	111	407	Putative sporulation-specific glycosylase YdhD OS=Bacillus subtilis (strain 168) OX=224308 GN=ydhD PE=1 SV=2
O32258	1.34e-23	1	301	30	328	Uncharacterized glycosylase YvbX OS=Bacillus subtilis (strain 168) OX=224308 GN=yvbX PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000031	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001893_01460.