CAZyme Information: MGYG000001922_00635

Basic Information

• Species: CAG-465 sp000433135
• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-465; CAG-465; CAG-465 sp000433135
• CAZyme ID: MGYG000001922_00635
• CAZy Family: CBM50
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
339		38180.31	6.8879

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001922	1374088	MAG	Denmark	Europe

• Gene Location: Start: 7802; End: 8821 Strand: -

Full Sequence      

MYNFKVVIDAGHGGEDPGAVANGVKEKDLTLMISEYMYEKFLEKGVDVVLTRSTDETLYP
SNRVNRILNAFGDGEDVIVISNHINSTSTPNSSEGSEVIYALRNESTLANNILKALESQG
QITRKVYQRASTTNPNKDYYFIHRETGNTEPIIIEYGYINNEKDLQRIQENYRKYVDAIV
DSIIATKSNKKYTKDYLVKPGDSLWSIANMYGTTVIELKALNNLQSDILSVNQVLKVPVT
DIFGITYIVKPGDNLWSIANKYGVSVASLKSYNNLKNDYIMIGQSLHIPLSNENKLYVVE
KGDTLWMIAKSNNTTVEKIKALNNLTNNYIYVGQVLKLR

Enzyme Prediction     

No EC number prediction in MGYG000001922_00635.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CBM50	247	289	6.3e-16	0.975

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK06347	PRK06347	2.16e-35	186	339	398	592	1,4-beta-N-acetylmuramoylhydrolase.
PRK06347	PRK06347	3.74e-35	188	338	325	523	1,4-beta-N-acetylmuramoylhydrolase.
cd02696	MurNAc-LAA	1.30e-33	5	184	1	172	N-acetylmuramoyl-L-alanine amidase or MurNAc-LAA (also known as peptidoglycan aminohydrolase, NAMLA amidase, NAMLAA, Amidase 3, and peptidoglycan amidase; EC 3.5.1.28) is an autolysin that hydrolyzes the amide bond between N-acetylmuramoyl and L-amino acids in certain cell wall glycopeptides. These proteins are Zn-dependent peptidases with highly conserved residues involved in cation co-ordination. MurNAc-LAA in this family is one of several peptidoglycan hydrolases (PGHs) found in bacterial and bacteriophage or prophage genomes that are involved in the degradation of the peptidoglycan. In Escherichia coli, there are five MurNAc-LAAs present: AmiA, AmiB, AmiC and AmiD that are periplasmic, and AmpD that is cytoplasmic. Three of these (AmiA, AmiB and AmiC) belong to this family, the other two (AmiD and AmpD) do not. E. coli AmiA, AmiB and AmiC play an important role in cleaving the septum to release daughter cells after cell division. In general, bacterial MurNAc-LAAs are members of the bacterial autolytic system and carry a signal peptide in their N-termini that allows their transport across the cytoplasmic membrane. However, the bacteriophage MurNAc-LAAs are endolysins since these phage-encoded enzymes break down bacterial peptidoglycan at the terminal stage of the phage reproduction cycle. As opposed to autolysins, almost all endolysins have no signal peptides and their translocation through the cytoplasmic membrane is thought to proceed with the help of phage-encoded holin proteins. The amidase catalytic module is fused to another functional module (cell wall binding module or CWBM) either at the N- or C-terminus, which is responsible for high affinity binding of the protein to the cell wall.
pfam01520	Amidase_3	1.46e-32	6	183	1	172	N-acetylmuramoyl-L-alanine amidase. This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls.
COG0860	AmiC	3.43e-25	4	183	43	224	N-acetylmuramoyl-L-alanine amidase [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QVK20078.1	1.85e-60	6	290	2	285
QIC05530.1	2.92e-36	6	180	85	249
AUM64330.1	6.49e-36	6	202	157	343
AGR47373.1	3.45e-35	6	180	158	322
QIC07671.1	1.30e-33	6	201	157	355

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5J72_A	2.69e-12	6	168	454	616	ChainA, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630],5J72_B Chain B, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630]
4UZ2_A	1.66e-07	196	238	5	47	Crystalstructure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_B Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_C Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ2_D Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4UZ3_A Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8],4UZ3_B Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8],4UZ3_C Crystal structure of the N-terminal LysM domains from the putative NlpC/P60 D,L endopeptidase from T. thermophilus bound to N-acetyl-chitohexaose [Thermus thermophilus HB8]
1JWQ_A	5.35e-07	6	164	4	151	Structureof the catalytic domain of CwlV, N-acetylmuramoyl-L-alanine amidase from Bacillus(Paenibacillus) polymyxa var.colistinus [Paenibacillus polymyxa]
5EMI_A	9.95e-07	6	183	7	170	ChainA, Cell wall hydrolase/autolysin [Nostoc punctiforme PCC 73102]
4XCM_A	1.44e-06	196	238	5	47	Crystalstructure of the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8],4XCM_B Crystal structure of the putative NlpC/P60 D,L endopeptidase from T. thermophilus [Thermus thermophilus HB8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P54421	3.98e-17	196	288	88	192	Probable peptidoglycan endopeptidase LytE OS=Bacillus subtilis (strain 168) OX=224308 GN=lytE PE=1 SV=1
O07532	3.20e-16	193	288	239	350	Peptidoglycan endopeptidase LytF OS=Bacillus subtilis (strain 168) OX=224308 GN=lytF PE=1 SV=2
P54525	3.58e-16	6	183	32	200	Uncharacterized protein YqiI OS=Bacillus subtilis (strain 168) OX=224308 GN=yqiI PE=3 SV=3
O31852	2.00e-15	196	288	159	268	D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS OS=Bacillus subtilis (strain 168) OX=224308 GN=cwlS PE=1 SV=1
Q49UX4	2.33e-14	186	288	19	129	N-acetylmuramoyl-L-alanine amidase sle1 OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=sle1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000041	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001922_00635.