CAZyme Information: MGYG000001931_01812

Basic Information

• Species: CAG-279 sp900548875
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-279; CAG-279 sp900548875
• CAZyme ID: MGYG000001931_01812
• CAZy Family: GH81
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1577		173621.53	4.2701

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001931	3207508	MAG	Denmark	Europe

• Gene Location: Start: 31422; End: 36155 Strand: -

Full Sequence      

MTSVWNVEGENLSGGAERNVLQGFLPHHYKNSTLDFSFTGYEYATPRGKMKLAAGNRFST
AYHFSGILPWFACPEEDSTLSHPYQRERMVSMIADYAAKGTFGADTYWGGKGLIQMALNM
TFALEMGEDALFEQCRSRLREAMEDWLTYTPGESQRFFARYSRWGALVGYDTSYDSDTFN
DHHFHYGYYAYAGAVLALVDDDFRAKYGEMLRLLAKDYANWERDDKRFPLFRTFTPWSGH
SYAGGLGNAGNGNGQESTSESMQAWGGMYLLGVALGDKAMRDAGIFGWVTESKGVAEYWF
DRDRENINRSLYTKPYNSNLTAAGIGWWTWFSGDPVWMHSIQWMPVSPCLDYLSENLDFA
RWDYETMWAGKEIGDWVADKNYAGSALSKESGLGNVVLSYLQRFDPDQAAQIFDEMWDAE
MPVARNTDTNGITYYITHSHRTYGDRDYTVNADITTSSAYCNADGQYTYVVYNPEDEERQ
VTFYRDGSELVSFKAPAGKLTVYKDAPQLSAVEIMNSESVVAPGKSLQLKAALLDQYGAT
TDGEVAWSVEGTGGTVDTNGLFTAGQQKGQTCKVVAVSGGLRAEKELRIDDEPVLHSAAI
EPMVDYAEAGTVLTFSLNAKDQYGDAFPVARQWRIKKDGNVVKNDSILDVQGVGIYTVEA
VADGGTVYSHDVYMTPPMENLALHKRVVVSSYENVGTVAESATDGDYSSRWGSQHNDDEW
IYVDLGASKYVSYVTLYWEAAYASLYEIQVSDDASHWTTVAEVSGGGGVENVNVGQSARY
VKMQGKGRATTYGYSLYELEVHGIDPESDPSELMGIEVTAPSLLIKEGEEVKLKAVGYDG
RGEKVDIQPQWSVEEGKGTVSDDGVYVPAGYGTSEVTASYDGKKATVQFVVEESIKPRSV
EIYPQSVELVKGESVAFSIEVFDQFSTACSSDGFVYACKAAPELMDGAVFNGLREGEYEI
IVSKGAVADTAYVKVGSYADVNLALNKPVTASSYENDLTLPEFVNDGDLSTRWGSSFEDN
QFITIDLEAPYVLNHIILHWNKDAYATDYRVEVSTDEETWHAVYQESGSTGGMRDITFAD
IAGQYVRIYCMARSSQYGSCLDEVEVFGKSCYENPVAIKVQVSLPDGAVAYIGEPVQLKA
VLTDQYGLEYAPQGSFVWEVSNGNATVDASGILTPVSEGECVVTVGYENLTDKMELRVLA
ERKMTSLQIFPPVAEIEVGVPLQLEAVVADQYGNTHKEICSWECSGGSCTDGAFVADEPG
YYQVTALSGDLKATATVEVVPMLTENIALNRPAEASSGTAGAANDDNTGTRWESDHVEGP
EWLMFDLQNAYHLMRSEIQWEAASAADYEIQVSLDGEHWQTLVAEKGLSGERTDRATVSA
IARYVRLWCTKRALPYGYSVWEWRLYGREMRAGEACEVVFVDAPAEIPADSESQFTVEVK
DAAGQAVTNPDLDWYVSGGGVIRTDGHFEAVVPGDYSVSAWSGLANATHDFSVIYLSGIA
RNEDTGVVITCLQNRLRIQSESAVRSVAVYSAAGSRLWTVGGLSANRYETSLDLSDGVYV
VAVQTENGYRIGKIMLK

Enzyme Prediction     

No EC number prediction in MGYG000001931_01812.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	3	418	5.5e-88	0.6286173633440515
CBM32	691	800	4.1e-23	0.9112903225806451
CBM32	1294	1403	4.3e-21	0.8790322580645161
CBM32	991	1104	7.5e-21	0.9193548387096774

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5498	Acf2	1.20e-41	25	413	340	729	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
pfam17652	Glyco_hydro81C	2.12e-30	107	370	32	300	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
pfam00754	F5_F8_type_C	6.58e-20	687	798	2	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	4.26e-18	988	1101	1	123	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	1.58e-17	1294	1402	8	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARU51487.1	2.32e-138	1	1108	482	1673
ALD12350.1	7.04e-136	1	1107	467	1660
QIS44472.1	7.04e-136	1	1107	467	1660
QIS41529.1	2.42e-135	1	1107	467	1660
QIS38766.1	3.35e-131	1	1107	467	1660

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FOP_A	6.09e-66	11	483	250	698	ChainA, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	3.46e-55	1	483	259	734	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5T4A_A	5.18e-55	1	483	279	754	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125],5T4G_A Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5UPI_A	8.44e-55	1	483	259	734	ChainA, BH0236 protein [Halalkalibacterium halodurans C-125],5UPM_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPN_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPO_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125]
5T4C_A	1.24e-54	1	483	279	754	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9UT45	2.48e-18	25	363	325	677	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
P53753	5.94e-18	5	348	675	1035	Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1
D4AZ24	1.05e-14	107	413	574	876	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
Q12168	2.13e-14	76	365	434	718	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1
Q09850	4.42e-14	126	353	399	631	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.998704	0.001183	0.000086	0.000006	0.000003	0.000048

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001931_01812.