CAZyme Information: MGYG000001946_00283

Basic Information

• Species: CAG-611 sp000434175
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; CAG-611; CAG-611 sp000434175
• CAZyme ID: MGYG000001946_00283
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
991		111507.3	9.2345

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001946	974231	MAG	Denmark	Europe

• Gene Location: Start: 55343; End: 58318 Strand: +

Full Sequence      

MKKTVNNEKKDKNIKRKININFKEKFKFKPIKKKDNKKININDIKEEEIENIDNLEGTIK
MKKVSVKKKKKKKVLKKPNENFKGKKVDKLFILILLALIVLIFGICYILFGKLISFVMTF
LFIVIMIFTQILDNTARDSKIRKIIKTIVIVGIVFCIVGVVLMTAFFIYIAAKAPEFDIE
KFDRNETTLIYDNKNELKATLGEEKREKVTYDEIPEVLINAIIATEDSRFFQHNGFDAPR
FIKAAIGQVLGRGGGGASTLTMQVSKNNLTNSDVSITRKFTDIYLSIFKIEKNLSKQQII
EYYVNNPYLGNYSYGIEQASLSYFNKHAYELNLAEASLIAGLFQAPGDYDPTVNPEYAKE
RRETVLNLMVRHGYITKEEAEIANNISIESILNANSNSTSIYQGYIDYVVKEAIKKTGLD
PYFYPMEIYTNMDTKKQEGVNNVMNEKTYTWPKNKPTLQAGVAAVESTSGKILAIGSARN
RKGERVLSYATDINKQIGSTAKPLFDYGPGMEYNNWSTYTIFKDEVYSYTGGGVMKNYDF
RYLGDITLRYALSDSRNVPALKAFQQVDNKKIYEFVTSLGIVPETTEGSTYLHEAHSIGA
FNGSNPLTMAGAYQAFSNGGYFYEPYSINKIILRDTGETFNYSSDKTKAMSDSTAYMITD
VLKDVAVQIGVRGAAGTPVALKTGTTNYDSKTSQQMGYPSSAAPDGWIVGYTPNIVMAMW
TGFDTNTKGEYLTSNQMTSHRNALYRALAKAVFDRTGQDFKRPSSVISVAIEKGTNPGLL
PSENTPSNMKVTELFKRGTEPTDISNAYISLENVTGLTSNYADGKITLTWNNVEIKTLNK
GEEERFGNFGYKIFVDGKYVGFTKDTTYSIETDNQYATYKVCTAYEKINTNIASGESITI
SPDVSFEYFGNDVTLHIGDSYVEDKLENIIKVYLEGKNITKDSTITIKMLNANNEVVETI
DTTKIATFKIEYHVKYKNASKTFTKNITVLE

Enzyme Prediction     

No EC number prediction in MGYG000001946_00283.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	197	370	5.2e-55	0.9774011299435028

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	1.07e-154	205	726	1	504	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	6.69e-144	136	803	6	650	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.17e-108	143	803	3	766	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	9.06e-64	195	370	2	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG4953	PbpC	2.90e-62	161	724	11	537	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AKS38635.1	6.56e-157	142	871	25	752
AXM90108.1	1.82e-156	142	805	25	687
AWI12666.1	2.63e-156	142	839	32	722
ASA95728.1	1.38e-155	142	871	25	752
ACJ33515.1	5.44e-155	142	871	25	752

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3DWK_A	7.74e-90	190	766	10	602	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
2OLU_A	4.04e-86	173	766	2	611	StructuralInsight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Apoenzyme [Staphylococcus aureus],2OLV_A Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus],2OLV_B Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus]
3ZG8_B	2.21e-56	289	770	2	479	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3ZG7_B	4.01e-52	289	770	2	479	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
2C5W_B	1.05e-47	400	723	1	334	PENICILLIN-BINDINGPROTEIN 1A (PBP-1A) ACYL-ENZYME COMPLEX (CEFOTAXIME) FROM STREPTOCOCCUS PNEUMONIAE [Streptococcus pneumoniae R6]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39793	4.37e-131	146	831	36	728	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
Q00573	5.43e-102	137	723	2	600	Penicillin-binding protein 1A (Fragment) OS=Streptococcus oralis OX=1303 GN=ponA PE=3 SV=1
Q04707	1.67e-97	137	723	2	599	Penicillin-binding protein 1A OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=ponA PE=1 SV=2
Q8DR59	1.67e-96	137	723	2	599	Penicillin-binding protein 1A OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=pbpA PE=1 SV=1
P38050	4.41e-74	178	766	39	610	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000071	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
90	109
113	135
148	170