CAZyme Information: MGYG000001951_00575

Basic Information

• Species: CAG-873 sp900548975
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-873; CAG-873 sp900548975
• CAZyme ID: MGYG000001951_00575
• CAZy Family: GH97
• CAZyme Description: Glucan 1,4-alpha-glucosidase SusB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
657	MGYG000001951_4|CGC1	72454.14	4.6962

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001951	2929441	MAG	Denmark	Europe

• Gene Location: Start: 49482; End: 51455 Strand: -

Full Sequence      

MKKYLSLIATAALLSACSEAPTVSSPDGTIKVDVSRDGSSLSYAVTVGGDTLIAPSHLGF
VAEGLTPAADAKMTVKHSSFDQTWTQPWGENKEIRNHYNEVAVTLTDAALEYTVRVRAFD
DGVAFRYEWNLASSDSVVVRDELTEFAFARPAMSWAIPANFDTYELEYRNVAINDVTDAN
TPFTFEVDSAGIFGSIHEAALYDYPEMTLVRTAGRTLKANLAPLPDGVKAYLPSAFATPW
RSIQLGKSACDLINSGLILNLNEPSKIADTSWIKPQKYVGIWWGMHLGTMTWTMGPRHGA
TTENAIRYIDFAAENNLQGVLFEGWNEGWDTWGGKQHFNYAKAYADFDIDSIAAYATSKG
IELWAHNETGGNIPEYEAAMDSTFAYYKSLGIHTLKTGYAGGYVGGQYHHSQYGVKHYQL
VVETAAKYQLMVDAHEPIKETGIRRTWPNMMTREGARGMEWNAWSAGNTPEYLCTLPFVR
LLSGPMDYTPGIFDINYERAKADPGRIEWNGRNDYCKINTTVARQIANWVIIYSPLQMAA
DLIENYEGQPAFQFFRDFNADCDWSQALQGEIGKYIAVVRRAGDNYYLGAGTDSEARTLT
VPLSFLGEGTYTATVYADDPADATKVVISSRDVTAADTLTVEMAAAGGQAVTFLPGR

Enzyme Prediction     

No EC number prediction in MGYG000001951_00575.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	11	653	1.4e-216	0.9920760697305864

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	2.46e-134	269	558	1	277	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	3.85e-78	25	264	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	1.62e-32	562	653	1	96	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASB37496.1	0.0	1	657	1	664
QQR08228.1	0.0	1	657	1	664
ANU64405.1	0.0	1	657	1	664
BBL01467.1	0.0	12	657	16	668
CBK63835.1	5.37e-314	13	655	20	668

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HQ4_A	1.34e-170	22	653	2	658	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	1.34e-170	22	653	2	658	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	3.77e-170	22	653	2	658	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
3WFA_A	1.07e-123	78	618	84	663	Catalyticrole of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron],3WFA_B Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron]
2JKA_A	1.10e-123	78	618	93	672	Nativestructure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKA_B Native structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKE_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKE_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKP_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482],2JKP_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
G8JZS4	3.75e-124	2	618	4	683	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
Q8A6L0	4.49e-57	22	618	24	623	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
D7CFN7	2.75e-38	24	657	44	627	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000063	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001951_00575.