CAZyme Information: MGYG000001952_01194

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium_A
• CAZyme ID: MGYG000001952_01194
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
319	MGYG000001952_64|CGC1	36506.82	5.1514

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001952	2903653	MAG	Denmark	Europe

• Gene Location: Start: 487; End: 1446 Strand: +

Full Sequence      

MNKMLYDLMDWAGIEEMVYSEARHPENLLGAHVVDGGVLVQAFIPTAESIEVKMGSGTYQ
MEMADEEGFFAVLIPRKTIAPYTLRITYDNETKAEIRDPYSFSNQITEQELKKFDAGVNY
EVYKKLGAHPMTVDGVKGVLFAVWAPCAMRVSVVGDFNLWDGRRHQMKLHEGYGVYELFV
PGLEEGALYKYEIKKMNGDPILKADPYANYAELRPNTASIVWDIEKYKWNDKAWMDKRAK
TDTKTKPMSIYELHLGSFMRKELQMDEAGNPIVGSEFYNYREIAPKLAEYVKKMGYTHVE
LLPVMEHPLDASWGYQVSG

Enzyme Prediction     

No EC number prediction in MGYG000001952_01194.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CBM48	125	210	2.3e-19	0.8947368421052632

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	1.05e-143	21	319	11	303	1,4-alpha-glucan branching protein GlgB.
PRK12313	PRK12313	1.23e-97	107	319	8	208	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	2.68e-80	106	319	6	202	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK12568	PRK12568	5.22e-74	29	319	30	307	glycogen branching enzyme; Provisional
PRK14705	PRK14705	3.62e-67	9	318	503	802	glycogen branching enzyme; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK76766.1	2.43e-166	1	319	1	321
CBL36572.1	2.43e-166	1	319	1	321
QIX91704.1	9.28e-163	1	319	1	323
ASN98288.1	6.99e-162	1	319	1	323
QRP36905.1	1.90e-161	1	319	1	323

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6KLF_A	1.19e-81	14	319	6	329	ChainA, 1,4-alpha-glucan branching enzyme GlgB [Crocosphaera subtropica ATCC 51142]
5GQU_A	2.33e-81	14	319	30	353	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR0_A	2.33e-81	14	319	30	353	Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	2.33e-81	14	319	30	353	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR5_A	2.33e-81	14	319	30	353	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8DLB8	3.46e-84	14	319	8	330	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q3SH78	1.79e-81	12	319	22	318	1,4-alpha-glucan branching enzyme GlgB OS=Thiobacillus denitrificans (strain ATCC 25259) OX=292415 GN=glgB PE=3 SV=1
Q1AZ86	9.61e-81	14	319	7	300	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q114I1	3.63e-80	14	319	8	330	1,4-alpha-glucan branching enzyme GlgB OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=glgB PE=3 SV=1
P52981	1.25e-79	14	319	10	332	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001952_01194.