CAZyme Information: MGYG000001963_00146

Basic Information

• Species: Phocaeicola sp000434735
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola sp000434735
• CAZyme ID: MGYG000001963_00146
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
829	MGYG000001963_1|CGC4	92599.76	4.9431

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001963	3288738	MAG	Denmark	Europe

• Gene Location: Start: 176101; End: 178590 Strand: -

Full Sequence      

MKGTVLSFVFMGLCGMMSAQSDVSPAIPRDNRMEQKIETLLRKMTLDEKVGQMCELTIDL
LQKRTNPFGSDPTKITVADVKRVLKTYALENEFKVGNEMPGKEVMMQIYMRVMGIESQKG
FQLNEAMLDSVIGKYKVGSILNVPNSIAQTPKKWQEIITRIQEKSMKEIGIPCVYGVDQI
HGTTYTQGGTFFPQGINMGATFNRSLTREGARISAYETRAGSIPWTYAPVVDLGRDPRWP
RMWENYGEDAYVNAEMGREAVLGFQGENPNEVDAYHVAACMKHFMGYGVPVSGKDRTPSS
ITLQDMREKHFAPYLEMVKAGALSLMVNSAMNNGLPFHANYELLTSWLKEDLNWDGMIVT
DWADIVNLYKRDHIAKDKKEAIKLAINAGIDMSMDPYSWDFCVLLKELVEEGEVPMTRID
DAVRRVLRMKYRLGLFEKPYNDYKDYPLFGSKEHAAVALQAAEESLVLLKNTDQILPLAK
GKKILVTGPNANSMRTLNGGWSYSWQGDKADMCAEEYNTILEAFVNKFGAENVIYEPGVT
YKQGGLYWEENTPDIKKAVEAAADADYIVACIGENSYCETPGNLTDLDLSVNQSDLVKAL
AATGKPIVLILNEGRPRILNEVESLAKVVVNIMLPGNYGGDALANLVAGEVNFSGKMPYT
YPKEINSLITYDYKPCEHIGTPMEGAYNYDAQVSVQWAFGYGLSYTSYEYSNLKVNKTKF
DSDDVLTFTVDVKNSGQMAGKESVLLFSSDLVASLTPDNRRLRAFTKINLQPGETKTVTL
DVKASDLAFVGYDGKWILEAGDFQIQVGNQVVNVTCNETYKWDTPNKTF

Enzyme Prediction     

EC	3.2.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	166	394	3.6e-64	0.9768518518518519

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.42e-144	37	808	38	751	beta-glucosidase BglX.
COG1472	BglX	4.89e-81	106	503	1	373	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	2.17e-63	130	429	25	316	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	1.56e-61	190	815	112	776	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.25e-48	466	705	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQA29841.1	0.0	2	827	3	829
QUT61352.1	0.0	2	827	3	829
QPH58960.1	0.0	2	827	3	829
QUT37180.1	0.0	2	827	3	829
BBK86655.1	0.0	2	827	3	829

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JP0_A	0.0	24	827	4	759	Bacteroidesovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus],5JP0_B Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus]
5XXL_A	2.80e-102	122	808	42	738	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	1.48e-101	122	808	42	738	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5TF0_A	1.82e-98	137	814	56	743	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]
6R5I_A	1.23e-97	136	808	45	719	ChainA, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5I_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXU3	0.0	2	827	5	781	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
Q23892	1.95e-145	37	810	80	814	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
Q56078	3.15e-103	162	808	95	751	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	8.58e-103	162	818	95	761	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
T2KMH0	1.11e-93	167	808	61	707	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000205	0.999199	0.000158	0.000154	0.000138	0.000131

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001963_00146.