CAZyme Information: MGYG000001967_00160

Basic Information

• Species: CAG-492 sp900557045
• Lineage: Bacteria; Firmicutes_A; Clostridia; TANB77; CAG-508; CAG-492; CAG-492 sp900557045
• CAZyme ID: MGYG000001967_00160
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
856	MGYG000001967_3|CGC1	98139.12	6.5848

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001967	1715002	MAG	Denmark	Europe

• Gene Location: Start: 13481; End: 16051 Strand: +

Full Sequence      

MYVFNKITVTPQLPERIKGLSTIANNLWWSWNSEFLRLFKEIDIDLWERVEKNPVKFLRL
VSQDKLNEILQNSEFLKKYDELLENHANYMSSKNTWFSKKYPNNKNDLIAYFSAEYGLDE
IMQIYSGGLGILSGDHLKSASDLGIPLVAVGLLYKNGYFHQKINAYGEQETEFKNIDVSN
LPITEVKDAKKDNILVSVNMPKGTLYLKIWQVNVGRVKLYLLDSDVEENIEEYRGITSTL
YGGNQETRIQQEIVLGMGGVKVLKILGLNPTIYHMNEGHSSFLILELINQIMQEKKVSFN
IAKDIVSSKTVFTTHTPVPAGNDIFPLSLVETYFDGIWEKYGISKNEFLKMGMAPNSEVE
NSEFNMGILALKVAGKKNGVSKLHGAVSRELFAEVWPNIIAHESPITYVTNGIHTCSWLA
PNLKKLYNKYLIPFWQDMIYDDNVWKNVKNIPDKDLWDAHIDRKRKLLALVKDNTAERLK
RNGYTYDQIAKIVSGLNPNALTIGFARRFATYKRATLIFRDLERITQILNDKNRPVQLIF
AGKAHPADKEGQELIKFIHEISMKPQFKGKVFLLEDYSIGMSRYLISGVDVWLNNPRRPM
EASGTSGQKASVNGVINFSILDGWWAEGYNANNGWKIGTNADYDNYEIQDNDDSESIYNT
LEKKIIPSYYNQNDKGFSNSWVNMMKESIITTGGKFSTARMLVDYVQKLYMPLCNLTNKY
YSNLADVTEFNEWKRKINESFDKIVITQEDTNADNETIDAGKSIKVRCKVKLPNISVDKI
QVQVYYGQIEDTGVVSNAVVIPMKLIESDEENRTYRFETDVELTTGGNYGYTFRVMPKHE
MILDAENLDLVKWIEK

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	122	743	1.9e-140	0.9198813056379822

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0058	GlgP	0.0	20	723	6	714	Glucan phosphorylase [Carbohydrate transport and metabolism].
TIGR02094	more_P_ylases	0.0	109	712	1	601	alpha-glucan phosphorylases. This family consists of known phosphorylases, and homologs believed to share the function of using inorganic phosphate to cleave an alpha 1,4 linkage between the terminal glucose residue and the rest of the polymer (maltodextrin, glycogen, etc.). The name of the glucose storage polymer substrate, and therefore the name of this enzyme, depends on the chain lengths and branching patterns. A number of the members of this family have been shown to operate on small maltodextrins, as may be obtained by utilization of exogenous sources. This family represents a distinct clade from the related family modeled by TIGR02093/pfam00343.
cd04299	GT35_Glycogen_Phosphorylase-like	0.0	20	800	1	776	proteins similar to glycogen phosphorylase. This family is most closely related to the oligosaccharide phosphorylase domain family and other unidentified sequences. Oligosaccharide phosphorylase catalyzes the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism.
pfam11897	DUF3417	1.20e-50	13	120	1	107	Protein of unknown function (DUF3417). This family of proteins are functionally uncharacterized. This protein is found in bacteria and archaea. Proteins in this family are typically between 145 to 860 amino acids in length. This protein is found associated with pfam00343. This protein has a conserved AYF sequence motif.
PRK14986	PRK14986	1.38e-15	126	623	123	676	glycogen phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUG58376.1	0.0	1	853	1	852
ABN51595.1	0.0	1	854	1	853
ANV76629.1	0.0	1	854	1	853
ADU74919.1	0.0	1	854	1	853
ALX08879.1	0.0	1	854	1	853

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4BQE_A	5.88e-16	116	623	153	734	Arabidopsisthaliana Cytosolic Alpha-1,4-glucan Phosphorylase (PHS2) [Arabidopsis thaliana],4BQE_B Arabidopsis thaliana Cytosolic Alpha-1,4-glucan Phosphorylase (PHS2) [Arabidopsis thaliana],4BQF_A Arabidopsis thaliana cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with acarbose [Arabidopsis thaliana],4BQF_B Arabidopsis thaliana cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with acarbose [Arabidopsis thaliana],4BQI_A ARABIDOPSIS THALIANA cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with maltotriose [Arabidopsis thaliana],4BQI_B ARABIDOPSIS THALIANA cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with maltotriose [Arabidopsis thaliana]
2FFR_A	3.86e-15	118	623	114	683	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
1ABB_A	5.09e-15	118	623	116	685	ControlOf Phosphorylase B Conformation By A Modified Cofactor: Crystallographic Studies On R-State Glycogen Phosphorylase Reconstituted With Pyridoxal 5'-Diphosphate [Oryctolagus cuniculus],1ABB_B Control Of Phosphorylase B Conformation By A Modified Cofactor: Crystallographic Studies On R-State Glycogen Phosphorylase Reconstituted With Pyridoxal 5'-Diphosphate [Oryctolagus cuniculus],1ABB_C Control Of Phosphorylase B Conformation By A Modified Cofactor: Crystallographic Studies On R-State Glycogen Phosphorylase Reconstituted With Pyridoxal 5'-Diphosphate [Oryctolagus cuniculus],1ABB_D Control Of Phosphorylase B Conformation By A Modified Cofactor: Crystallographic Studies On R-State Glycogen Phosphorylase Reconstituted With Pyridoxal 5'-Diphosphate [Oryctolagus cuniculus]
1C50_A	5.10e-15	118	623	113	682	IdentificationAnd Structural Characterization Of A Novel Allosteric Binding Site Of Glycogen Phosphorylase B [Oryctolagus cuniculus]
1GPA_A	5.14e-15	118	623	125	694	STRUCTURALMECHANISM FOR GLYCOGEN PHOSPHORYLASE CONTROL BY PHOSPHORYLATION AND AMP [Oryctolagus cuniculus],1GPA_B STRUCTURAL MECHANISM FOR GLYCOGEN PHOSPHORYLASE CONTROL BY PHOSPHORYLATION AND AMP [Oryctolagus cuniculus],1GPA_C STRUCTURAL MECHANISM FOR GLYCOGEN PHOSPHORYLASE CONTROL BY PHOSPHORYLATION AND AMP [Oryctolagus cuniculus],1GPA_D STRUCTURAL MECHANISM FOR GLYCOGEN PHOSPHORYLASE CONTROL BY PHOSPHORYLATION AND AMP [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9YGA7	1.92e-224	9	853	8	825	Maltodextrin phosphorylase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) OX=523849 GN=malP PE=1 SV=1
Q7U078	1.33e-191	1	851	1	860	Glycogen phosphorylase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgP PE=3 SV=1
P9WMW0	5.28e-191	1	851	1	860	Glycogen phosphorylase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=glgP PE=3 SV=1
P9WMW1	7.46e-191	1	851	1	860	Glycogen phosphorylase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=glgP PE=1 SV=1
O66932	2.61e-171	15	713	4	687	Glycogen phosphorylase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=glgP PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000042	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001967_00160.