CAZyme Information: MGYG000001977_02607

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000001977_02607
• CAZy Family: GH18
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
660	MGYG000001977_128|CGC1	73959.74	4.5644

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001977	4322077	MAG	Denmark	Europe

• Gene Location: Start: 3333; End: 5315 Strand: -

Full Sequence      

MKYMNHVKSGLLAMAFACAGLCFVGCEDDVVINTGNSDRLDTVDGVYGYVKSAAGARELT
SISVFGDKVATGHLYFELSKAAEQDVTVTFKVDEEVLEAYNKKNGTDYKMYPVDKLSLAN
GGTAIIKAGEQKSASVELNINAGGTIGQTYAVAVSASANNGVEVSTNNQNYIYLVKPLAA
IPESISKGDMLTHCFVEVNDENIVNMGEYTMKSSGKPFFDVVSIFAANINVDSKTGRVHV
FCNDQVSFLLRNVDKYIRPLQAKGIKVVMTILGNHDEAGMGNLSEAAAKDFAKELKAYLD
IYGLDGIDFDDEYTSYNNSNPGSGFEKRSRANFARLVYECRQVFGSKLIGVYEYGSLDSP
DGEVEGVKVGDIVDYMTYGYYQNQNTNGAFGREESHFENLPKSKYAPLPWKINEELNGGW
SGFDKSKFQKVKDEGYGIQMFYNPKPLAYQYYTLLSEISKVLFDDEVEWSGKYWSRTGEA
YQGKMMGYEDLVGEWKVTSSNSLFTYVDEEGNPRWWDWKGSQELEKNVIIEKDEKGTGYV
VYNWGTFPEITGKYPMHCDYYNGALLIYPQTIHEGDAEDPATYKMHFGTYSKIFQWKAYP
DYDNDYMDGAIAPNGDMHIYGLGNRWAINPYKYVGGEIETDLFPDVPYFVSENYTMQKVR

Enzyme Prediction     

EC	3.2.1.96

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	215	538	7.6e-17	0.7533783783783784

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06542	GH18_EndoS-like	1.81e-54	194	463	5	255	Endo-beta-N-acetylglucosaminidases are bacterial chitinases that hydrolyze the chitin core of various asparagine (N)-linked glycans and glycoproteins. The endo-beta-N-acetylglucosaminidases have a glycosyl hydrolase family 18 (GH18) catalytic domain. Some members also have an additional C-terminal glycosyl hydrolase family 20 (GH20) domain while others have an N-terminal domain of unknown function (pfam08522). Members of this family include endo-beta-N-acetylglucosaminidase S (EndoS) from Streptococcus pyogenes, EndoF1, EndoF2, EndoF3, and EndoH from Flavobacterium meningosepticum, and EndoE from Enterococcus faecalis. EndoS is a secreted endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes the glycan on human IgG between two core N-acetylglucosamine residues. EndoE is a secreted endoglycosidase, encoded by the ndoE gene in Enterococcus faecalis, that hydrolyzes the glycan on human RNase B.
pfam08522	DUF1735	1.00e-19	49	163	6	120	Domain of unknown function (DUF1735). This domain of unknown function is found in a number of bacterial proteins including acylhydrolases. The structure of this domain has a beta-sandwich fold.
pfam00704	Glyco_hydro_18	8.57e-11	224	381	26	178	Glycosyl hydrolases family 18.
cd02871	GH18_chitinase_D-like	1.60e-05	257	320	66	127	GH18 domain of Chitinase D (ChiD). ChiD, a chitinase found in Bacillus circulans, hydrolyzes the 1,4-beta-linkages of N-acetylglucosamine in chitin and chitodextrins. The domain architecture of ChiD includes a catalytic glycosyl hydrolase family 18 (GH18) domain, a chitin-binding domain, and a fibronectin type III domain. The chitin-binding and fibronectin type III domains are located either N-terminal or C-terminal to the catalytic domain. This family includes exochitinase Chi36 from Bacillus cereus.
smart00636	Glyco_18	4.25e-04	284	381	88	187	Glyco_18 domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT60732.1	0.0	1	660	1	660
QQA29261.1	0.0	1	660	1	660
BBK87383.1	0.0	1	660	1	660
QUT98017.1	0.0	1	660	1	660
QUT35299.1	0.0	1	660	1	660

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6T8I_A	3.51e-58	62	467	37	430	Crystalstructure of wild type EndoBT-3987 from Bacteroides thetaiotamicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],6T8K_A Crystal structure of Bacteroides thetaiotamicron EndoBT-3987 in complex with Man9GlcNAc product in P1 [Bacteroides thetaiotaomicron VPI-5482],6T8K_B Crystal structure of Bacteroides thetaiotamicron EndoBT-3987 in complex with Man9GlcNAc product in P1 [Bacteroides thetaiotaomicron VPI-5482],6T8L_A Crystal structure of Bacteroides thetaiotamicron EndoBT-3987 with Man9GlcNAc product in P212121 [Bacteroides thetaiotaomicron VPI-5482],6TCW_A Crystal structure of Bacteroides thetaiotamicron EndoBT-3987 with Man5GlcNAc product [Bacteroides thetaiotaomicron VPI-5482],7NWF_A Chain A, Endo-beta-N-acetylglucosaminidase F1 [Bacteroides thetaiotaomicron VPI-5482]
6TCV_B	6.56e-56	62	467	37	430	Crystalstructure of Bacteroides thetaiotamicron EndoBT-3987 in complex with Man9GlcNAc2Asn substrate [Bacteroides thetaiotaomicron VPI-5482]
3POH_A	2.36e-54	78	467	52	430	Crystalstructure of an endo-beta-N-acetylglucosaminidase (BT_3987) from BACTEROIDES THETAIOTAOMICRON VPI-5482 at 1.55 A resolution [Bacteroides thetaiotaomicron VPI-5482]
2EBN_A	3.60e-52	186	476	6	287	CRYSTALSTRUCTURE OF ENDO-BETA-N-ACETYLGLUCOSAMINIDASE F1, AN ALPHA(SLASH)BETA-BARREL ENZYME ADAPTED FOR A COMPLEX SUBSTRATE [Elizabethkingia meningoseptica]
1EDT_A	9.71e-40	195	443	14	245	ChainA, ENDO-BETA-N-ACETYLGLUCOSAMINIDASE H, ENDO H [Streptomyces plicatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P36911	2.09e-51	164	476	35	337	Endo-beta-N-acetylglucosaminidase F1 OS=Elizabethkingia meningoseptica OX=238 GN=endOF1 PE=1 SV=1
P04067	1.53e-38	195	443	56	287	Endo-beta-N-acetylglucosaminidase H OS=Streptomyces plicatus OX=1922 PE=1 SV=1
P80036	3.41e-31	195	443	60	289	Endo-beta-N-acetylglucosaminidase OS=Flavobacterium sp. (strain SK1022) OX=148444 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000001	0.000297	0.999751	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001977_02607.