CAZyme Information: MGYG000001981_00752

Basic Information

• Species: CAG-617 sp000431275
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; CAG-617; CAG-617 sp000431275
• CAZyme ID: MGYG000001981_00752
• CAZy Family: GH2
• CAZyme Description: Evolved beta-galactosidase subunit alpha

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1343		151481.48	6.2339

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001981	2121938	MAG	Spain	Europe

• Gene Location: Start: 85; End: 4116 Strand: +

Full Sequence      

MMRKLTLGLLAAALSWPAYADVQPLAGFRYAAEEAPTGREWQSPEDLALNKEQPRARFFS
FADTESARKVLPEHSSYWMSLDGTWKFNWAKNPDERPKTFFDPAFDTSGWDDVQVPMCWN
VVGIQKDGSLKYGVPIYSNQRVIFQHELKVDDWRGGVMRTPPQDWVTFKDRNEVGSYRRT
FTVPAEWDGREVYINFDGVDSFFYLWINGHYVGFSKNSRNLAAFNITDFLQKGENTVAVE
VYRSSDASFLESQDMMRLPGIFRSVSLTSKPQVHVRDLVVIPDLDNDYVNGSLTITADVR
NLSKKKAKGYKMTYTLYANPLYSDETRLVDGVGAEVEVNEVASGHSEAARTVLYVTNPMK
WSAEEPWRYTLIGELKDKKGRTVETVSATVGFRKVEIKDTKAEDDEFGLAGRYFYVNGRT
VKFKGVNRHETSADRGHAITHEQMEKEIMLMKRANINHVRGSHYPDNPYWYYLCNKYGIY
LEDEANIESHEYYYGKESLSHPVEWRAAHIARDMEMVHANVNDPAIVIWSLGNEAGPGEN
FVHAYNAIKAFDTSRPVQYERNNSIVDMGSNQYPPINWMWQAASGKLPIKYPFHISEYAH
SMGNAVGNLVDYWNAIESTNYICGGAIWEWIDHGLYYYDKTSGRRFIAYGGDFGDKPNDG
IFCMDGLMNADLTPKPQYYEVKKVYQNVDVLPVDMTKGQIRVANENYFIPIDRNYEMTWT
LFKDGLPIDSGNAFRGPRMELGPRDTLSYSIPYDYASLEPQSEYFVKVQFRLRQDMPWAK
KGYVQMEEQLLVKEATGWPAIASVAKGGKLSVADEKDFITVKGDQFTARFDKNTGTIYDL
TYGTDKVIADGNGPKLDAYRAQVDNDNWAWNAWIQNGLHNMAHRALSSDSYTRKDGAVVV
SFNVESQAPYKSTIRGGAAAGPYKLTEHKDQPTGPDDFKFNSQVTWTVYADGSIELSSSI
TSNNSELDLARIGYSFQVDPKYSRYTYYGRGPLNNYNDRKTSQFIERYESTVKDQFVNHG
KPQDMANREEVRWAALTDASGHGIQFVSTGRLSTSVLPWSSLELMLAPHPYQLPESHTTH
VNLDAAVTGLGGNSCGQGSPLPKDHVKAVPHIVGFLLRPTNGADLTTNAAVTSSGDQTPI
ISRDRKGNMTVSGTREGATIMYAVGKEKAKAYEGTRNLRTGGTVTAWYEDMPQVKMTMTF
PRIENIPMVVVETTSQESGEGNASHLVDGNPNTYWHTMYSVTVAQYPHSVTFDASEEKNI
KGFTYLPRQDSRNGNVKGYKVQVSKDGKTWGDPVAQGEFANDQKLKRIMLAKPVKARFVR
FTATSSQDGQDFASGAEFGVLEE

Enzyme Prediction     

No EC number prediction in MGYG000001981_00752.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	69	1005	3.5e-205	0.9720744680851063
CBM32	1212	1338	5.1e-28	0.9193548387096774

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	0.0	41	1129	4	1009	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	0.0	39	1092	15	999	beta-galactosidase.
COG3250	LacZ	6.44e-125	71	1003	5	804	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	6.03e-83	414	688	8	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
smart01038	Bgal_small_N	1.05e-73	822	1117	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIM35762.1	0.0	24	1343	24	1342
QCQ49582.1	0.0	2	1343	1	1341
QCQ33874.1	0.0	2	1343	1	1341
QRM72246.1	0.0	2	1343	1	1341
AUI47491.1	0.0	2	1343	1	1341

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	8.37e-192	40	1118	3	980	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	8.60e-192	40	1118	4	981	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3BGA_A	9.21e-172	40	1119	9	1000	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3DEC_A	2.94e-169	40	1098	6	976	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	2.03e-122	39	1093	14	996	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O52847	3.21e-191	40	1102	19	1015	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q56307	4.71e-191	40	1118	4	981	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
P06864	6.30e-149	41	1129	4	1009	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4
Q9K9C6	4.30e-148	47	1106	16	998	Beta-galactosidase OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=lacZ PE=3 SV=1
A1SWB8	5.42e-144	39	1093	11	1008	Beta-galactosidase OS=Psychromonas ingrahamii (strain 37) OX=357804 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000440	0.998726	0.000222	0.000213	0.000186	0.000163

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001981_00752.