CAZyme Information: MGYG000001988_02172

Basic Information

• Species: Marvinbryantia sp900550755
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Marvinbryantia; Marvinbryantia sp900550755
• CAZyme ID: MGYG000001988_02172
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
811	MGYG000001988_20|CGC1	94426.87	4.9374

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001988	3989920	MAG	Spain	Europe

• Gene Location: Start: 4286; End: 6721 Strand: -

Full Sequence      

MLQQFLTEGWQLRKAGEELQPAVVPGTVYTDLLRNGNMEDPFWKDNEIRALALMDFDYEY
QAVFDCDAQMLKKDRVLLHFDGIDTIADIYVNETKVGSAYNMHRVWEYDVKALLKETGNA
LRVVLHSPTKYIQEEFKKCRTLGSEDAMDGFVHLRKAHCMFGWDWGAHLPDAGIFRKVSL
IGIDRARIDNVYVAQKHENGQVTLEFKVETETAAAGAKLHYAVEITAPSGEVLRAENSPK
ELLITEPEFWWPNGYGSQPLYGVKVTLFSGDEALDIWERRIGLRTLTMHIEKDQWGESFA
HEVNGITVFAMGADYIPEDHLLGRVTPETTKKLLLQCKAANFNTIRVWGGGYYPEDWFFD
LCDELGLMVWQDFMFACAVYELTSEFKANIRQEFIDNVKRIRHHASLGLWCGNNEMEMFV
GENHHWVTKKTEVRDYLIMYEELIPDVLKEYDPQTFYWPASPSSGGSFDEPNSPDRGDVH
YWTVWHGNKPFSEYRKYFFRYASEFGFQSFPSKKTVETFTDDPRDMNIFSYIMEKHQRQY
GANGKIMNYMQQTYLYPSGFEQAIYASQLLQADAIRYGVEHFRRNRGRCMGAIYWQLNDC
WPVVSWSSIDYCGRLKALQYYAKRFFAPLMLSCQEEGMMSQEADMNREHFVFEKSIRLNV
ANETRRAEKVHVRWALRNADVSVVSQWEEDVEVPALTSVWLDKMELPEADIFSQYVSYEM
EKDGKIISSGTVIFSYPKYFRYEDPKLSYEIDGDEIIVRAHAYAKSVEILNENEDLILED
NYFDMNAGEKRVKVLSGDIEKLRLRSVYDIR

Enzyme Prediction     

No EC number prediction in MGYG000001988_02172.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	733	5.6e-100	0.7353723404255319

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.85e-93	1	632	10	635	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	5.49e-16	60	415	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam17786	Mannosidase_ig	1.99e-11	656	739	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam17753	Ig_mannosidase	9.90e-09	744	809	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam00703	Glyco_hydro_2	1.06e-08	186	284	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJU13790.1	0.0	1	811	1	823
QQQ93614.1	0.0	1	811	1	823
ASU28856.1	0.0	1	811	1	823
ANU76049.1	0.0	1	811	1	823
QBE97789.1	0.0	1	811	15	837

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	3.18e-146	6	811	8	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	3.26e-146	6	811	8	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	1.64e-145	6	801	11	823	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.73e-145	6	801	13	825	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.78e-145	6	801	13	825	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0CCA0	3.97e-107	3	797	6	828	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
Q4WAH4	1.13e-106	2	797	4	830	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
A1D911	3.07e-106	2	797	4	830	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
B0YBU9	4.28e-106	2	797	4	830	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A2QYN2	6.19e-104	6	636	9	663	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001988_02172.