CAZyme Information: MGYG000001998_00696

Basic Information

• Species: Lachnospira sp900552795
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnospira; Lachnospira sp900552795
• CAZyme ID: MGYG000001998_00696
• CAZy Family: GH28
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1410		153221.67	4.6072

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001998	2300661	MAG	Spain	Europe

• Gene Location: Start: 135326; End: 139558 Strand: +

Full Sequence      

MKKGFKKAAAAGLTAIMLLSSVNFSGFTVNAALPTTDGHDKYVNKAAFDVIGSKDFGSKD
LDGPLFDKTKGSQNITELQVPTLAYDDTSVGLVWQKPEKYDNVADYKIWINNTYAGTARE
NFKVNAEWTAKYMEAFYDYYDKNGDVDMTKVDIHSYRATGLSPDTEYTFKVVAVDAAGNE
LGTAQTIKQRTAKTPETVNILDYGAKTSEGYVTYNDEINAFIEANTKAIQAAIDACPEGG
RVVIPEGTFVSGALWLKSNMTLEINGTLWASPNSDHFEIGFLMYPFYTDTRVWGLVNATS
ADENAPLENIRITGTGTLYGNGWKYGEGNTIKGDGFGRYTYSESADVDTSAPGNEKYTLP
RWVAGSNTKVYNFGVQAADTCTKWLKNCTNPDGSRKYSDELVDSLADRIAKNNGDKLSEI
ETQDLKNAYAARASLLIMKNVTNVYVGDITVENPSNHSVNILDSRNISATNVKVFSYDGN
NGDGLGFGCSQNVICWNNFTDTGDDNLGFGASVGEGARDSEIQTNSELWMFNNYLHEGHG
GLAAGSHTGNGIQDVLFEDTVMNHIDAAFRFKSAPTNGGFIANITMRDCAVAGCDQGFVL
TTSYSDPNSASSTEAAEVAEFYNFAAVNISVYEVKYNTIQVLADVDTVNNPQKPWHTHHN
LYFQDVTFGNVGTNGSYKNKNGWETLIGCENAVFYNVKTNSYSSKAVSKGTDTAWNNLQY
CKNIRFQGTTMDSLNADKEKMQNIMSGITVTDNTVKAENYVPPVGDYTVGATIKSWSAKD
IAATKSNKSGTVGTADEGLAANCDDGNTWKTGSDGAASWEGENAGTTYACQSGGNKAMDA
DGVTLSKGTIPVSGAYLTYEATQDGVLSVAEKTLATKRFYIVDSDGKVCAEHYNANPKGG
ATEYKILTTPVTKGKTYYIYANGATNNIFKVWLSEKIDGAEKPQAKDGKNVKLTWNAVDN
GTEKVFYGVDVYAGGQKVDARDGIKDTSVIIDRLSSGVDYTFKVYVSENGDSANSMNLTG
WNKTYIGETSFKTDGEKDTEAIKAPADSTVSAASTVYTHTQGSWESLSRQDKRVRGYDVY
VNGKLTKTIYNYQIPKYETADTVSKQIGRMKAGIDNEIKIVAFTDAGVEYAYTPVTVKTL
ENYDYKAPVFAETAKVTATKQANGDVVLTWNAAVDDTAVNGYRVYVDGKPVYANAEDVFN
PVNADKTTDKTTYTISGLDLTKDHTFTIQAGDTWWKAAQTMGSYDKMAYYNWTVKGISTE
LKAEPPVVEKADYTAVDNAIKAANALVPDDYVDFTGVTNAINAVVRDLPKSDQAKVDAMA
QAILDAIGALEKKPEATEPDTEPDTENDAGADDTKPDIKPDDGNTPAAPDETPKTGDSTS
ILMVMMTFIISGGLLVIIWDRRKKKYKFDK

Enzyme Prediction     

No EC number prediction in MGYG000001998_00696.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	236	635	2.5e-41	0.8307692307692308

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	4.97e-46	80	588	3	392	Polygalacturonase [Carbohydrate transport and metabolism].
pfam00295	Glyco_hydro_28	4.38e-07	435	585	87	230	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
smart00060	FN3	1.29e-04	86	178	13	81	Fibronectin type 3 domain. One of three types of internal repeat within the plasma protein, fibronectin. The tenth fibronectin type III repeat contains a RGD cell recognition sequence in a flexible loop between 2 strands. Type III modules are present in both extracellular and intracellular proteins.
cd00063	FN3	3.25e-04	86	178	13	81	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.
NF033847	MCP_Sipho	6.81e-04	1274	1380	7	118	major capsid protein, Siphoviridae type. This protein is a phage major capsid protein, as reported in primary sequence submissions of a large number of Siphoviridae, many of which have hosts in the Mycobacterium and Gordonia genera of bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOV20136.1	0.0	1	1337	1	1159
SET92437.1	1.00e-218	66	1256	96	1060
AOV08612.1	5.52e-111	69	1288	29	996
BAB85762.1	2.78e-109	63	1230	23	927
AIF50363.1	5.54e-99	78	1232	42	887

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2UVE_A	1.50e-91	77	671	40	564	Structureof Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVE_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVF_A Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica],2UVF_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica]
3JUR_A	3.19e-14	198	588	28	345	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]
5OLP_A	9.63e-06	225	590	60	356	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P15922	1.03e-85	77	633	35	524	Exo-poly-alpha-D-galacturonosidase OS=Dickeya chrysanthemi OX=556 GN=pehX PE=1 SV=1
P20041	5.53e-07	209	605	56	400	Polygalacturonase OS=Ralstonia solanacearum OX=305 GN=pglA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000245	0.999076	0.000179	0.000172	0.000160	0.000140

TMHMM  Annotations     

start	end
1380	1399