CAZyme Information: MGYG000001998_00797

Basic Information

• Species: Lachnospira sp900552795
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnospira; Lachnospira sp900552795
• CAZyme ID: MGYG000001998_00797
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1340	MGYG000001998_4|CGC1	142580.33	5.5731

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001998	2300661	MAG	Spain	Europe

• Gene Location: Start: 22474; End: 26496 Strand: +

Full Sequence      

MQKAKKRVLALGLTLMMILSSIIPGVGNAAAVNAATTALAISESSGWFESAYVEWKPVSG
AKRYDVYVKASSETTYKRLDDELVRQYPGYWRADAVGLAAGTYDMKVIAVMSDGTTSAEK
KGLVVKPYDRSGFAFSKDSTLKTASGAYNDDGTLKPNAQVIYVTKDTAKTVTATLKNGSK
TVTATGIQAILKAKDKDPETPLAIRIIGCVSDKDVDYFGSSSEGIQIKGAKAYQPMNITI
EGIGEDATVNGFGFLVRNSGNVEFRNFAILNFMDDGISMDTANTNIWIHNMDFFYGSVGG
DADQAKGDGTVDLKGDTQWTTVSYNHFFDSGKMSLCGMKSETGPNYITYHHNWFDHSDSR
HPRIRTMSVHIYNNYYDGNAKYGVGAAQQSDAFVESNYFNNCKYPMLSSMQGSDIADGAV
GTFSGEDGGIIKAYNNKIVGAKRLVYANSASGTTGAADSTQFDAVLVNSRTETVAGSYKG
VKGGHVYNNFDTKVDLGVSASDIDDVNMIPTIVKAEAGRMNGGDLKFTFDASEDSNYAVI
PELKNKVVNYKTTLYSVGGSVDGTAPEPEQPEKPTEPETEAPTQKPTEKPTQPETEAPTE
KPSVSGKAVVHNFTENGKTSDFYTITGNLSTSKGTVSYDGHKLTSCLKMESSTSVKFTAA
EKGTLTLVFVEKNKEGAVKVDGKSYNSNANGIVEVAVSAGSHTITKANTSNLFYMAFAGS
TAEVPTEKPTEAPTQKPTEPETEAPTQKPTQPETEAPTEKPSVSANKVVHNFTESGKTSS
FYTITGNLSTSKGTVSYDGLKLTSCLKMESSTSVKFTAAEKGTLTLVFVEKNKEGAVKVD
GKSYNSNANGIVEVAVSAGSHTITKANTSNLFYMSLTQGNSEQPTEKPTEPPTEPEKPTE
PEKPIDPTPAAGDIYVSPSGKSSASGTESDPMDLVTAINKIAAGKTIWMQAGTYRYTNTI
LIPESNSGKSGSYKSIKSKGGDVIIDFAGMAEAGSNRGIVLDGSYWYLYNIDITNAGDNG
MLLSGDNNIIELCQFYKNHDSGLQISRYNTNYATKDLWPSNNLIKNCTSYDNKDEATSEN
ADGFAAKLTCGEGNVFDGCIAYCNSDDGWDLYAKPATGSIGVVTIKNCVAFGNGKTTDGQ
GSANGDMNGFKLGGSNGACPTPHVVTNCLAFFNGACGFTDNGNGGAIKMTNCTAANNGIY
SSKANFMCYRTSQSALYSNLLSFASNGKLGTDKFKGAVEKTLYFNTSGSAYYWVDAKDTS
SFTGAGIKYGTPNAKSVSNSDFVSTTLAGYNASKGTYATNYHEILRNADGSVNMNGLFQL
KSSSPLSNSGTNGRALGAAL

Enzyme Prediction     

No EC number prediction in MGYG000001998_00797.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	910	1325	3.7e-126	0.9866666666666667
PL1	234	401	1.3e-42	0.8168316831683168
CBM77	769	872	2.1e-41	0.9805825242718447
CBM77	610	713	3.8e-41	0.9805825242718447

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	4.73e-52	106	405	18	279	Pectate lyase [Carbohydrate transport and metabolism].
pfam18283	CBM77	1.10e-41	768	874	3	108	Carbohydrate binding module 77. This domain is the non-catalytic carbohydrate binding module 77 (CBM77) present in Ruminococcus flavefaciens. CBMs fulfil a critical targeting function in plant cell wall depolymerisation. In CBM77, a cluster of conserved basic residues (Lys1092, Lys1107 and Lys1162) confer calcium-independent recognition of homogalacturonan.
pfam18283	CBM77	3.73e-41	608	715	2	108	Carbohydrate binding module 77. This domain is the non-catalytic carbohydrate binding module 77 (CBM77) present in Ruminococcus flavefaciens. CBMs fulfil a critical targeting function in plant cell wall depolymerisation. In CBM77, a cluster of conserved basic residues (Lys1092, Lys1107 and Lys1162) confer calcium-independent recognition of homogalacturonan.
smart00656	Amb_all	1.14e-33	237	403	17	190	Amb_all domain.
pfam00544	Pec_lyase_C	5.63e-19	235	399	33	211	Pectate lyase. This enzyme forms a right handed beta helix structure. Pectate lyase is an enzyme involved in the maceration and soft rotting of plant tissue.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACR72246.1	2.01e-303	3	1246	7	1163
ABX41986.1	4.93e-294	1	1338	1	1163
ACR72247.1	7.44e-274	3	1319	7	1043
ADL51369.1	1.66e-259	1	1339	11	1250
AOR96287.1	8.52e-253	36	1337	52	1092

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RU4_A	1.21e-32	915	1331	18	380	ChainA, Pectate lyase [Dickeya chrysanthemi]
5FU5_A	1.57e-29	610	718	9	114	Thecomplexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition [Ruminococcus flavefaciens]
3VMV_A	1.49e-25	192	401	38	248	Crystalstructure of pectate lyase Bsp165PelA from Bacillus sp. N165 [Bacillus sp. N16-5],3VMW_A Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165 in complex with trigalacturonate [Bacillus sp. N16-5]
1VBL_A	2.54e-18	233	400	129	331	Structureof the thermostable pectate lyase PL 47 [Bacillus sp. TS-47]
5OLQ_A	8.73e-17	915	1198	6	320	Rhamnogalacturonanlyase [Bacteroides thetaiotaomicron],5OLQ_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLQ_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLS_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P22751	5.81e-35	911	1244	388	687	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
P0C1A6	1.58e-33	915	1331	43	405	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1
P0C1A7	1.03e-31	915	1331	43	405	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
Q8GCB2	1.08e-24	178	408	59	281	Pectate trisaccharide-lyase OS=Bacillus licheniformis OX=1402 GN=pelA PE=1 SV=1
Q65DC2	1.08e-24	178	408	59	281	Pectate trisaccharide-lyase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi04129 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000464	0.998638	0.000220	0.000277	0.000191	0.000162

TMHMM  Annotations     

start	end
9	31