CAZyme Information: MGYG000002001_00277

Basic Information

• Species: CAG-177 sp000431775
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; CAG-177; CAG-177 sp000431775
• CAZyme ID: MGYG000002001_00277
• CAZy Family: GT2
• CAZyme Description: Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
889		103755.35	7.115

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002001	2259768	MAG	Spain	Europe

• Gene Location: Start: 344410; End: 347079 Strand: -

Full Sequence      

MSDFQYKVSVVIPVYNCAEHLERCAKSLTKQTIRKSQLEVIFVNDGSKDGSGEICKRLSE
QYSFVKFFDKENGGVSSARNTGIRLAKGKYIMFLDADDTLSKNTLKAVTGFFDKHYDEVD
LVTYKIIPYWKKQRYALHSRFKVLGDSGVYDLNEGQNCYICQTTMNICIKNLGEGKTPLF
DEKLKFHEDINYCCGIVRDKMKIGYCKEAEYYYLRHPSSTTDRRGFAYYIFEDTMAMWER
LFAPYGDKVPRYYQAIYLNDLNWKTIQDRLLPYYYSEAKFDKSVERILTLVKKIDDDVIL
ERPGMDLYHKHFLINFKQSGKVALQCDEDALRLVYNGETTSLLEQGETVYEAEKVCIVAD
RLKVRGGRFIFDGFLKSPIFMYCGKPEVNLIIDGESRPLELTHSENEHYKAGIRTGTFWR
VQFEFDCAKVNDFRIEVRVNGVGYGTTFYYGPHSSIAQHRRFKGFSARGLVCREANGAFT
VRHFPSFGAVTLMLRLLISNFFCYLRIKPAVIPNRITGYLHRAKRRSVWIYLDRYGVFDN
AYDQFKHDIGKEDGVERYYILNECDLDKLNERFTTEEKEHIVLFGSHRHKELYFECDKLI
TSFSNLSNVCPFSAGPMRWYADLTKYELVYLQHGILHASLRKMYSKECTPIDKIVVSSEF
EVKNLTENYGYSESDLIRSCMPRYDGMEISGKKKNRLLFSPSWRSNLIGPLVNNERKEEP
EAFLNSPFCKQVTEFLNSERLHDLLVKNDLYLDFQNHPIFRCYNSLLNITNDRVCTDSSA
RQDEYRLMITDYSSIVFDSVYLGCPIIYFVPDYAEFLAGVSHNYRKLDLPLEEGFGPFTE
DADSLIDRLEECIAAGFVPQEPYRSRMESFFLYRDDKCRDRLYDSLMTK

Enzyme Prediction     

No EC number prediction in MGYG000002001_00277.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	9	138	4.2e-32	0.7647058823529411

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd00761	Glyco_tranf_GTA_type	2.28e-30	10	123	1	112	Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.
pfam00535	Glycos_transf_2	9.54e-30	9	141	1	131	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
PRK10073	PRK10073	1.76e-28	1	252	1	260	putative glycosyl transferase; Provisional
COG0463	WcaA	1.11e-24	7	282	4	275	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
cd06433	GT_2_WfgS_like	1.22e-23	9	189	1	169	WfgS and WfeV are involved in O-antigen biosynthesis. Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUN13088.1	1.89e-193	1	887	1	872
QTU84178.1	3.92e-178	9	886	14	886
ANE23153.1	1.45e-176	8	888	7	880
QEO63827.1	2.58e-161	3	889	2	869
QCU20573.1	3.64e-161	3	889	2	869

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HEA_A	6.32e-18	8	98	7	95	CgTstructure in hexamer [Streptococcus parasanguinis FW213],5HEA_B CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEA_C CgT structure in hexamer [Streptococcus parasanguinis FW213],5HEC_A CgT structure in dimer [Streptococcus parasanguinis FW213],5HEC_B CgT structure in dimer [Streptococcus parasanguinis FW213]
5TZE_C	1.65e-17	7	213	2	202	Crystalstructure of S. aureus TarS in complex with UDP-GlcNAc [Staphylococcus aureus],5TZE_E Crystal structure of S. aureus TarS in complex with UDP-GlcNAc [Staphylococcus aureus],5TZI_C Crystal structure of S. aureus TarS 1-349 [Staphylococcus aureus],5TZJ_A Crystal structure of S. aureus TarS 1-349 in complex with UDP-GlcNAc [Staphylococcus aureus],5TZJ_C Crystal structure of S. aureus TarS 1-349 in complex with UDP-GlcNAc [Staphylococcus aureus],5TZK_C Crystal structure of S. aureus TarS 1-349 in complex with UDP [Staphylococcus aureus]
3BCV_A	3.54e-17	7	98	6	95	Crystalstructure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3BCV_B Crystal structure of a putative glycosyltransferase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5TZ8_A	5.77e-17	7	213	2	202	Crystalstructure of S. aureus TarS [Staphylococcus aureus],5TZ8_B Crystal structure of S. aureus TarS [Staphylococcus aureus],5TZ8_C Crystal structure of S. aureus TarS [Staphylococcus aureus]
2Z87_A	9.64e-15	8	114	376	480	Crystalstructure of chondroitin polymerase from Escherichia coli strain K4 (K4CP) complexed with UDP-GalNAc and UDP [Escherichia coli],2Z87_B Crystal structure of chondroitin polymerase from Escherichia coli strain K4 (K4CP) complexed with UDP-GalNAc and UDP [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P46918	6.07e-80	4	887	6	891	Minor teichoic acid biosynthesis protein GgaB OS=Bacillus subtilis (strain 168) OX=224308 GN=ggaB PE=3 SV=1
P71059	1.33e-23	8	222	5	220	Uncharacterized glycosyltransferase EpsJ OS=Bacillus subtilis (strain 168) OX=224308 GN=epsJ PE=2 SV=1
P46917	1.72e-22	9	325	3	327	Minor teichoic acid biosynthesis protein GgaA OS=Bacillus subtilis (strain 168) OX=224308 GN=ggaA PE=3 SV=1
A0A0H2URH7	1.03e-19	7	151	6	146	Glycosyltransferase GlyA OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyA PE=3 SV=1
A0A0H2UR96	2.54e-18	8	172	5	144	Glycosyltransferase GlyG OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyG PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000077	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002001_00277.