CAZyme Information: MGYG000002019_00959

Basic Information

• Species: Eubacterium_R sp000431535
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp000431535
• CAZyme ID: MGYG000002019_00959
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
752		85762.03	6.8731

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002019	2121271	MAG	Spain	Europe

• Gene Location: Start: 146531; End: 148789 Strand: +

Full Sequence      

MRKCIEINQGWYFSKDSSVFPDALPNGWEQVDLPHTWNGKDGQDGGNDYYRGKCYYAKEL
LCSDFSGGEEYYLQFDAVNSSAEVYFNGKKQITHHGGYSAFRVKIEKNDIKDKNLLVVSA
DNSPNNFVYPQNADFTFYGGIYRSVKIIAVPKVHFSLDYFGAPGIAVTPKVNGSSADILV
KAYTNSQAEVLFEILDHSGNTVCSQTVSGKDPECSFHMDSVHLWDGVQDPYLYTASASIV
QKDHNGDKITAKFGCRSFSFDPDKGFFLNGRSYPLRGVSRHQDRPDIGNALKYEHHKEDI
ELICEMGANTVRLAHYQHAQEFYDLCDAHGLIVWAEIPYISRHMPNGRQNTVSQMTELIC
QNYNHPCIVTWGLSNEITMGGAADKDLLDNHRVLNDLVHKMDKTRPTTMAVVTMCDPSEE
ITKISDILSYNHYFGWYGGNVNMYGPWFDEYHKKYPDRVIGISEYGCEALDWHTSNPEQG
DYTEEYQAFYHEELIKQISKRPYLWATHVWNMFDFAADARAEGGENGMNHKGLVTFDRKY
KKDSFYAYKAWLSDKPFVHICSKRYTDRVEDETLITVYSNLPYVELYANGECIEKQDKGE
LPFFKFKVKNVGETKLLAKCGEYTDESLIRKVDVFNEKYRMKEEGAVINWFEITTPPGRY
SVNDTIGDIISTFRGKIFAVRLLLMISKAFSAGKDKKKQKDGASIAGFKINKTMLGIAKG
FTLKRALMMMGGKFSKEDILKINSMLNKIKKK

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	597	5.6e-95	0.6715425531914894

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	1.09e-67	50	551	63	592	beta-D-glucuronidase; Provisional
COG3250	LacZ	9.56e-53	1	562	9	611	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	5.82e-29	263	551	5	297	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	6.01e-27	68	405	125	470	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.41e-18	140	406	208	484	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBJ55484.1	0.0	1	752	1	752
AEN97389.1	0.0	1	751	1	755
CED93924.1	0.0	1	749	1	750
QJA08636.1	5.34e-317	1	749	1	751
ASM70256.1	4.23e-309	1	751	1	737

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MVG_A	0.0	1	751	24	777	Crystalstructure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_B Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_C Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus]
6MVH_A	0.0	1	751	24	778	Crystalstructure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_B Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_C Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_D Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis]
7KGZ_A	1.19e-248	1	751	1	733	ChainA, Beta-glucuronidase [Roseburia hominis],7KGZ_B Chain B, Beta-glucuronidase [Roseburia hominis]
6MVF_A	1.49e-178	1	669	24	705	Crystalstructure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_B Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_C Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_D Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_E Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_F Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6]
3CMG_A	3.51e-130	1	596	3	615	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	9.02e-94	7	602	56	683	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.89e-48	6	612	18	607	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P26257	7.47e-40	1	407	1	413	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
A7LXS9	1.98e-37	2	619	42	723	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.12e-35	8	599	52	670	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002019_00959.