CAZyme Information: MGYG000002049_01195

Basic Information

• Species: CAG-485 sp900556595
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485; CAG-485 sp900556595
• CAZyme ID: MGYG000002049_01195
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1257	MGYG000002049_86|CGC1	137652.42	5.0821

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002049	1913681	MAG	China	Asia

• Gene Location: Start: 132; End: 3905 Strand: -

Full Sequence      

MNKQLTALALTALLSVPAGAQQLREGYVDWGIRGPEFPEALAKWERGQKWTDDDNFFISR
VKPHTRFRNEATQVNPELTKDFDKNLIFWVPINGEVNNALPDGKFDCEVFPMWSYVTHYG
NWSSRLVRIPAAFIDAAHRNGVPVSCVATPPWGQLTDTWKKAFNSMIATGADKMADFMAW
FGVDGIGYNSEFSTKKALVADLLKYNENLRAGLRKRGMEQGEIIWYDGTSESGSIRFDNG
LGSHNAACFGDSATPRSVLFFNYNWNRKSLLGNSSAYAKILKRSPLDLYCGINMQGKEPR
EGSGDIWPLLAQYHLSIGLWGAHAENMFFESRMEKGPDPLQRQRAYLDRVNTWFTGGTHN
PVTSPDLNNSLIVSAENEKFAGMSKMMSARSALGWDLSEEPFLTNFNLGNGRFFNFRGQR
QNTLEWYNIGIQDHLPTWQWWWSRKFLGRDAASAAGIGLKAEFSWDDAWLGGSLLRIYGT
NSADEYLHLFKTDFLLSSEDNITVRYKLAGGKTNMKLALSAKGAEEKEISLSSMLSDGTQ
PIGEWQTRVLNVGRDLPELSGKTLAMVALRFNDASGLDLRLGEFAITRGDKGATYPPMPE
ITKAEVLHNSRHGVDAKVIFNMINGVTKHQCYNTDVATSLFKLYAQQDGCEPIMMGMTSS
WAGLVFSVPVNPEGGKTLRLGVSALSLDMTNETPISWSEPMDISTGYTIDDEIASDKEVY
APGEAIGVGYVDPMHQGGEFTLTDDSGNVVGSAKNALRLELAEGLAAPGNYTLTVVGTDN
SSDAAVLTTRTFPRYIQVVPASAGAVPQVEEFTTAKGETEASSQPGENVDLKASVSAADG
EMSRGVQVGKNGVGFKAADAGIDGSGSFSVAFWFRADSFKNRSAQLLNIRDRMQKWYANM
QGWFLNSVKEDGTMDTFTIRGNSENLSYNFDKVRLEPKVWYHIAYVFDMEKEGGVLPSLY
INGEKQEVTSWSIGNEQKKGSDLDYAGGMYNWSVGNVVAVGGFLHNSGSALGRIDNLSVW
NKALDAEGVKVAMGSLAQGASKPEGLAGWYDFENDAAADYLFSDAAGGTCKAGAHDYIPT
EWEGQGSLDWRAPEYVAGCPWLDNNGYKLSTHWALSLDGTEVAAGDAKDALPKSVKMPND
NRDCVLTLKASNAHGISAREIRVKVGTGSVEEIAGAAPENLNVGPNPFDSYFQVEATEAG
NYEFVLYDASGAACGRWTRKMAAGETATIAPAVSAGLYVLTVSRDGNRLAPRRLLKR

Enzyme Prediction     

No EC number prediction in MGYG000002049_01195.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	103	414	3.5e-59	0.9492063492063492

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	4.24e-43	37	587	35	550	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	1.13e-40	113	441	30	334	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	2.58e-35	104	411	4	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
pfam13385	Laminin_G_3	1.88e-08	864	1030	15	151	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
smart00560	LamGL	2.08e-06	866	1024	1	130	LamG-like jellyroll fold domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ13703.1	3.80e-309	1	1257	1	1247
QUB57152.1	5.35e-296	20	1249	20	1236
QUB59112.1	5.35e-296	20	1249	20	1236
QUB60779.1	9.11e-296	20	1249	20	1242
QUB72358.1	3.62e-295	20	1249	20	1242

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VTF_A	2.05e-35	37	593	17	530	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
3FHA_A	3.48e-35	37	507	12	451	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHQ_A	4.64e-35	37	507	12	451	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C7A1	1.33e-13	109	480	149	462	Cytosolic endo-beta-N-acetylglucosaminidase OS=Gallus gallus OX=9031 GN=ENGASE PE=1 SV=1
Q8NFI3	2.74e-12	110	519	152	511	Cytosolic endo-beta-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=ENGASE PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000298	0.998934	0.000202	0.000191	0.000180	0.000158

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002049_01195.