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CAZyme Information: MGYG000002124_00331

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Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species CAG-110 sp900763965
Lineage Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-110; CAG-110 sp900763965
CAZyme ID MGYG000002124_00331
CAZy Family GH165
CAZyme Description hypothetical protein
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
477 MGYG000002124_9|CGC1 55145.14 5.286
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000002124 2646709 MAG Mongolia Asia
Gene Location Start: 9716;  End: 11149  Strand: +

Full Sequence      Download help

Enzyme Prediction      help

No EC number prediction in MGYG000002124_00331.

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH165 44 432 2.9e-59 0.5984251968503937

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
cd08995 GH32_EcAec43-like 5.78e-05 177 293 97 236
Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 (FosGH2). This glycosyl hydrolase family 32 (GH32) subgroup includes Escherichia coli strain BEN2908 putative glycoside hydrolase Aec43 (FosGH2). GH32 enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC GH32 family also contains other fructofuranosidases such as inulinase (EC, exo-inulinase (EC, levanase (EC, and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC, fructan:fructan 1-fructosyltransferase (EC, sucrose:fructan 6-fructosyltransferase (EC, fructan:fructan 6G-fructosyltransferase (EC and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize.
cd18607 GH130 4.51e-04 98 252 27 171
Glycoside hydrolase family 130. Members of the glycosyl hydrolase family 130, as classified by the carbohydrate-active enzymes database (CAZY), are phosphorylases and hydrolases for beta-mannosides, and include beta-1,4-mannosylglucose phosphorylase (EC, beta-1,4-mannooligosaccharide phosphorylase (EC, beta-1,4-mannosyl-N-acetyl-glucosamine phosphorylase (EC, beta-1,2-mannobiose phosphorylase (EC 2.4.1.-), beta-1,2-oligomannan phosphorylase (EC 2.4.1.-) and beta-1,2-mannosidase (EC 3.2.1.-). They possess 5-bladed beta-propeller domains similar to families 32, 43, 62, 68, 117 (GH32, GH43, GH62, GH68, GH117). GH130 enzymes are involved in the bacterial utilization of mannans or N-linked glycans. Beta-1,4-mannosylglucose phosphorylase is involved in degradation of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine linkages in the core of N-glycans; it produces alpha-mannose 1-phosphate and glucose from 4-O-beta-D-mannosyl-D-glucose and inorganic phosphate, using a critical catalytic Asp as a proton donor.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
QNM12975.1 4.55e-156 1 477 1 474
QGK04006.1 1.46e-145 1 477 4 479
QJS36974.1 1.46e-145 1 477 4 479
QEL28519.1 2.45e-134 2 469 8 457
QFX85916.1 1.13e-130 3 477 61 538

PDB Hits      help

has no PDB hit.

Swiss-Prot Hits      help

has no Swissprot hit.

SignalP and Lipop Annotations help

This protein is predicted as OTHER

1.000060 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000002124_00331.