CAZyme Information: MGYG000002125_01005

Basic Information

• Species: UMGS1795 sp900555125
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-552; UMGS1795; UMGS1795 sp900555125
• CAZyme ID: MGYG000002125_01005
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
608		70868.89	6.4002

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002125	1699850	MAG	Mongolia	Asia

• Gene Location: Start: 1777; End: 3603 Strand: +

Full Sequence      

MKKIEKNKSTDRKNGVIPVFYACDDNFIAFAAVSLRSLLDNCAKTRRYDVHFLVTAVSDE
NRKEILSMKTDFCDVTFDDVTPYLKELTSDLPIRDYYSKTTYYRLFIADMFPEYDKVIYI
DSDTVVEKDISELFDTDIDGMYAGAVHERVMSHNDTFGAYVEQVLGIDKYAYFNAGVLLI
NVARYREQNILKKFGELLGVYDFVVTQDEDYLNLLFKDRVKLLHGKWNAEIYDGIPCKER
EVAIFHYIMTSKPWHYKNCEYADHFWKYAKKTSYFELLQKQAENYSDADRERDDLCGKNL
VLLAEKEIAREDNYLKQLRKKQDAGRVAVLGKIEELEKSGVFDVDVEQDPPTKPLPDKVD
YLRKNVLAKLKARHAFYVARKFLHGMIENDEFIIDKIEGGENLRSVEGGAILTCNHFNAF
DSFAMQLAYEQADTKKHKFYRVIREGNYTSFGGFYGYLMRNCYTLPLGSDMKHMKMFTCA
VSEILKNNGFVLVYAEQSMWWNYRKPKPLKKGAFSMAATNGVPVVPCFITMRDTDKLDEN
GFKVQEYTIHIGKPIYPDENKSIAQNISYMMAENAVEWQQTYERFYGVPLRYDCHEQPFV
LREQKQKA

Enzyme Prediction     

No EC number prediction in MGYG000002125_01005.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	9	257	8.2e-56	0.9105058365758755

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	7.57e-60	17	256	1	248	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
pfam01501	Glyco_transf_8	4.42e-49	18	256	1	250	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
COG1442	RfaJ	3.63e-45	17	288	3	285	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
cd00505	Glyco_transf_8	2.38e-28	19	256	4	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
PRK15171	PRK15171	1.80e-20	7	256	16	272	lipopolysaccharide 3-alpha-galactosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQA01163.1	1.98e-204	14	593	4	605
QNL97928.1	1.19e-66	9	314	5	312
APR07562.1	3.91e-60	14	304	3	294
QOP49966.1	3.91e-60	14	304	3	294
QOJ84102.1	3.91e-60	14	304	3	294

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U4B_A	7.01e-23	14	281	299	596	ChainA, WbbM protein [Klebsiella pneumoniae]
1SS9_A	4.72e-21	19	255	3	253	ChainA, alpha-1,4-galactosyl transferase [Neisseria meningitidis]
1G9R_A	6.37e-21	19	255	3	253	ChainA, GLYCOSYL TRANSFERASE [Neisseria meningitidis],1GA8_A Chain A, GALACTOSYL TRANSFERASE LGTC [Neisseria meningitidis]
5GVW_A	7.23e-13	101	254	87	235	Crystalstructure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVW_B Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVW_C Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVW_D Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae]
5GVV_A	1.70e-12	101	254	87	235	Crystalstructure of the glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVV_F Crystal structure of the glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P43974	1.39e-23	19	261	41	284	Putative glycosyltransferase HI_0258 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0258 PE=3 SV=2
P25148	1.70e-20	12	274	2	276	General stress protein A OS=Bacillus subtilis (strain 168) OX=224308 GN=gspA PE=1 SV=2
P19816	5.78e-13	19	255	30	273	Lipopolysaccharide 1,3-galactosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaI PE=3 SV=4
A0A0H2URJ6	3.96e-12	101	254	87	235	Glycosyltransferase GlyE OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyE PE=1 SV=1
Q9AEU2	3.02e-11	98	268	90	253	Probable glycosyl transferase Gly OS=Streptococcus gordonii OX=1302 GN=gly PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002125_01005.