CAZyme Information: MGYG000002133_00598

Basic Information

• Species: Prevotella sp000436915
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp000436915
• CAZyme ID: MGYG000002133_00598
• CAZy Family: PL11
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1495		160525.81	5.7271

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002133	2840923	MAG	United States	North America

• Gene Location: Start: 29430; End: 33917 Strand: -

Full Sequence      

MIKKLLFAAALMAVAGGAQAQRQTDKLDRGLIAMKVSGGVYVNWRIPAEEYYDVTYNVYR
NGMKLNDKPLTVSNFTDKSGTLANTYTVSAIIKGKEQAQCKPASVWASSYKEIKLKHEGI
KSTLVPNDACCADLDGDGQLDMLMKFDNASEGAAGYPKEGYQGEYSIFEALKLDGTRLWW
VNCGPNMGDFQNNEQNIVAYDWDGDGKAEVVMRAADGTTIHCADGSTYVVGDASKNYRSG
TKSGQWFMCTGAEYLVYMDGVTGKPYECKEYPLKRLEDGETSLESAWGDGYGHRCTKHFF
GAPYLDGKKPSIFLARGIYTRHKMIAFDVDPATHTLKERWRWFNNTNGPWKGQGYHNYAI
ADVDMDGRDEIVFGSMVIDDNGKGLSTTGFGHGDAQHVGDLNPYIHGLEQFACNEDEEGF
NYRDATTSKVYASQLKVGSDIGRCICGNFTNNFPGCIGFAYGNPVSTVANKASAELSAPG
VAYNFNIYWDGDLLSETFNYVAGKNTEGGIYKYGQSGPIYTCQGSLTNNDTKGTPCYQGD
ILGDWREEIIMRTADNNIRIYSTPTPTEHRNYNLWFDHQYRNAMVWQMCGYNQPPHVSYF
MGELEGITIAPPPLTTTGRKEVADGGTIGSSLDGEHVLVFDNANTSVNIADGAKPSVLTF
NVPTWVQGSAPSEASAKSYPINTTTYTCTVNGGGLAGDARLVKQGDGILTLPKADFKHTG
NTDIWAGVVNFDGTMLNSPLWLNRFAELNSNGGKFSSIKMEYDAKLRPGCADTKGTITTD
NLSLGFGSRVVFDIYDDLSSDFIQGKVLNIETKDWQFGPQYLTPVFEFNNHGTNGLAAGK
YLLATFDKVEGDVSVIKLEGLDNTRKSHLALEGNNLYLVVEGVRDASTVVWTGAESATWD
VANTENFAMASDATKANFVNGDKVVFNDNAAIKNVVLNSDIEADSVIFDNTAAYNLSGDG
AITGNTVLVKRGTGTTTIRTDNTYTGGTRISGGVLNVNALASDVKNSGNLGANVVLANKM
VIENGATLRTAAAVTTNSPIKFETEAGGIIENPNDFTANKTLSGTVAYKKGGGTLILTNN
NTSLNKLVVVAGTVKNQAITIPAKMVELQGGTLTESSSTSYAISVAKGKSATWKLAERNT
YTNKITGEGTLSIYCPLVSGGSWMAPRTHVKCNMSEFEGTLKPQMPYKDNRFTLDNSYGL
PKATLDIPEGMEVQNTGKVFAIGKVTGTGALGGLVDFGNGVSGYNTWKVGNETNYRWSGK
VTGTSTAFVKIGSGKLTAGAGWDNTGSVKVAEGELCLSSGNVIGTGAVTVDKDARLSGVT
GTTALTNSSFTINGELVVGAFANATSGKINFGGKNVTISSTGKYVVGKGSYSNTTIENVN
TLTINGTIEVVLASSYTPKDGDVLTLWTANKFAGTPNYVLPNLPLGMAWDMSKISEGKLT
IVSDPTAIGVVPFGAKYGHNDIYDLKGQLVRKNATSTEGLPSGVYFRNGKKIVVK

Enzyme Prediction     

No EC number prediction in MGYG000002133_00598.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	22	603	3.1e-162	0.9777015437392796

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	5.50e-169	25	599	2	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
pfam18370	RGI_lyase	1.02e-35	22	106	1	86	Rhamnogalacturonan I lyases beta-sheet domain. This is the beta-sheet domain found in rhamnogalacturonan (RG) lyases, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 carrying this domain, such as YesW (EC:4.2.2.23) and YesX (EC:4.2.2.24), cleave glycoside bonds between rhamnose and galacturonic acid residues in RG-I through a beta-elimination reaction. Other family members carrying this domain are hemagglutinin A, lysine gingipain (Kgp) and Chitinase C (EC:3.2.1.14).
PRK15319	PRK15319	1.39e-04	959	1397	681	1068	fibronectin-binding autotransporter adhesin ShdA.
PRK15319	PRK15319	0.004	1233	1339	1430	1533	fibronectin-binding autotransporter adhesin ShdA.
PRK15319	PRK15319	0.008	959	1336	744	1085	fibronectin-binding autotransporter adhesin ShdA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIM09959.1	0.0	11	1486	1	1525
AGB29439.1	9.99e-318	1	1449	1	1461
BCS86257.1	3.43e-284	24	880	2	866
AUZ37627.1	2.24e-136	20	613	38	615
AMA51411.1	1.87e-135	20	613	32	609

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z8R_A	2.42e-135	20	613	1	578	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
4CAG_A	3.60e-126	22	603	9	577	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2ZUY_A	1.71e-120	21	623	5	610	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	3.24e-134	20	613	38	615	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	7.40e-120	21	623	5	610	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000583	0.997768	0.000983	0.000238	0.000214	0.000188

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002133_00598.