CAZyme Information: MGYG000002135_01301

Basic Information

• Species: Anaeromassilibacillus sp002159845
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Anaeromassilibacillus; Anaeromassilibacillus sp002159845
• CAZyme ID: MGYG000002135_01301
• CAZy Family: GH97
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2492	MGYG000002135_11|CGC1	273456.2	3.9555

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002135	2740278	MAG	United States	North America

• Gene Location: Start: 76690; End: 84168 Strand: -

Full Sequence      

MKKKAWRSLLSLLLAFTMVCSIWTPAFAKSEATEEWDPSAEYELLTVLKDAGIEVYSGNT
NPGATVDLWWNYHDDALYWRLIPDSDGEYYRIQPKNTNATVLMPIDSAAVEGTELTVGNI
DDADDSQWWKIVPAETEGQYKLLNKASEGTAPVYIALEDDAVDDGTPVILSDGTTASSYW
NISKVEVAPPEPVDPVVNVSAGKTLLNVGESTTLQATALDEYEESLAIDEITSSNPDVVE
VTQEGSEYTITAKANGVASIVASATLSDGSSVEGYTPIIVGTEPIDGAIYQLTAPTSEVS
ALFAYDAAYGVASYAALNGDSVVVSTSPTGIVTSEGDFRSGLEFVDVEETSGTEDYDLFG
AKVKHVNASYKQMTFTFEKEGASYAIILRVFDDGVAFRYSINGDESTDLSISSEHTGVRL
PNGAYTWAMNYSQSNEDVEIEHPSITELNYRYSMPLLYKTEDDVFALVNEADLNGTYCGA
QMTGDGTGLLRIVFTPEQTDDVETTLPFASPWRYVVIGDLQTITNNTLAESLSPESKVED
TSWIEAGAVDWTWLNGDLRHDQLPDGVTFETAGLEYYKKYVDFAVEMGWKYQLLDEGWQP
RGNTAAGEHEYEGYYDWMPELIDYAAEKGVGLIVWARKANLTDDAYRQKMFKEWADMGIK
GIKPDFFDSQDQETIQLIEKMMEETAEYHLLINVHGAGKPTGERRTWPHAIAREAVRGAE
AYAVDNIDTWGAIMNAHHNCSLPFVRGAVGPMDYTPMVSYGANDNLTNHNPGRFTVAHMT
ALPVIYECGLQCLADKPDVYRAHPGYEHYFKNMPSEWDEGILIDGYPGDYVNMARRNGDE
WYQGIICDGKLDATFPLNFLGDGEYVAYIYKDSPEVLEAGPAAYDQFGRHNQDGSNLVVD
EETVNSSIVEEIKVVTKDDTLTIPLAETGGAGIHYVPKDKVQEEAPAITEAKLITDRLIE
LDWDQDVTGADEKASFQVMVNGQEAELNDYGYNAGNGALYFDQMTTLELKEVPAEDAQIT
VQVVGNVTNELDEAADTEKVYTVEVAPFYTQFVEADCGITVKSSDAVEPSTLQAAADMVD
VMLSERSDIAEKMIENGAELAVYGQGESAYSIPEHRATYDPDMYYVEGYGGMVGNPVASI
YDANVLRLLRQDSPNHYTSYENESVLVHEFGHSVHLLGVDSIPELKEEFDAVYASAQAKN
LWPDSYAISNREEYFATCTAIWFNAMSESNDGSWDGVRGPVNTREELQTYDVDMYNFLAK
IYPETEAVSEAWANVPDNFHPSDEPQQPTWNTESDYKIKTVLNGSCMESNSNNSSAGAII
DLWWDFDGEAEYWRLIPDSNNEYYMIQLQSATSTVLMPTGSNVAEGVALTLGATDTANDA
QWWKIVPAEQAGQYRLMNKASEESPLYIALENDATMDGTLVILENDAAPSSVWTIEEQIR
NENYHEADPDAIYLSDLEWINAVDSWFLVRKDQTVNQTEISWNENGETVIYEKGIGTFAP
SQVTFDITGLHAETFKAIGCFPAGRGSCEFIVLADGEPILTKTLSYDGNQITPDNGALEV
AIPEGTKILTLYANGGSSTTWADARLYCDEEAQEDLARVTARTEETWMQVGETSAINVAG
YIGRVGWGGLQNIKADLTNATITYSSSDESVARVENGAIVAVADGTATITCNVELDGVTQ
STTLDVIVGNGTEDSWTAVSPDGSIKTLFTLRDDGSVVFATSRDGNTVIGASPTGLVTNL
GNFRTGLTFVDSKTETVTDSYDLIGAKKSHVDATGTELTMSFEKDGVTYRIVSRVYDDGM
AFQYQIEGQDGEELTISSEATGFLMPDGSVAQTMDFDKANEERYYQKEIEELEGGYCMPL
LYETVNGDWALLSEAALTPQYSGGQIVGHDSGMLDVVFSPEQFSDIVTTAPFVSPWRFMV
IGDPADIAENTLAETLNPDCAIEDTSWIVPGAVDWTWLNGDLRHDNLPEGTTWEETAPEL
YKQYIDFAVEMGWKYQLLDEGWQPRVYNRGNDLYDGYYDWMQEVLDYAEEKGVGLIVWAN
KRDLDTPEERERIKEWADMGIKGIKPDFFDSQSQEIIQLLDILTKEAAENHLLINIHGAG
KTTGERRTYPNAICREAVSGGESCWNDAYFDCMLPFTRFAVGGADFTPMASYGNTFGNKT
VTQAHLVAETIMFECGIQTMADKPAVYRAHVAYENLFKNIPTSWEDSILLDGDPGHYVNM
ARTTGDDWYVSLLANEARTAAFNLDFLDDGATYTAYIFRDSDDPMNDDTSKAIITETMTV
TKGQAMSIDLKETGGAVVKLVKTADWNELNAALTEAKSLNSSNYTAASWSAVEAAITAAE
QLPETADQATIDAATKAIKDAIANLARRPSNSGSSGSSSSNSGNPMGSDGVVVAVGTGAA
TAQPRVISDTTIDFTVKSGSAYCFKMTVVNGNNLMPSFTVGNGNVLKTQFVAQIGNDYYF
RVWAIGTPGQSTGVYTTLPGQSAVKHCTVTIG

Enzyme Prediction     

No EC number prediction in MGYG000002135_01301.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	1694	2300	2.7e-152	0.9793977812995246
GH97	290	877	5.8e-152	0.9239302694136292
CBM51	1453	1586	2.1e-16	0.9402985074626866

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	9.70e-69	540	804	1	268	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam10566	Glyco_hydro_97	6.72e-56	1944	2190	1	267	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	9.66e-47	1700	1938	1	234	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14508	GH97_N	4.81e-37	310	534	16	234	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	4.54e-26	2204	2301	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUO18240.1	6.62e-127	1693	2324	27	650
AEE97714.1	2.45e-114	286	937	8	592
ATB36826.1	1.12e-100	288	939	23	593
ADO69318.1	1.33e-99	287	939	23	595
QLH24877.1	5.85e-98	281	937	35	620

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	2.56e-60	321	880	36	610	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	2.69e-59	321	880	50	624	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5XFM_A	5.25e-55	1701	2260	22	609	Crystalstructure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_B Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_C Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_D Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron]
5HQ4_A	1.46e-39	1697	2299	2	657	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	3.47e-39	1697	2299	2	657	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
D7CFN7	1.09e-85	270	930	15	615	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
Q8A6L0	2.09e-59	321	880	57	631	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	1.99e-37	285	884	18	696	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000702	0.907950	0.090413	0.000417	0.000262	0.000218

TMHMM  Annotations     

start	end
5	27