CAZyme Information: MGYG000002135_01403

Basic Information

• Species: Anaeromassilibacillus sp002159845
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Anaeromassilibacillus; Anaeromassilibacillus sp002159845
• CAZyme ID: MGYG000002135_01403
• CAZy Family: CBM51
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2040		221844.63	4.0171

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002135	2740278	MAG	United States	North America

• Gene Location: Start: 33111; End: 39233 Strand: +

Full Sequence      

MKRKTVRSFIAVALALQMVAAGATPVFAAQAAQVVPAANHAVTAVMEDEEGDIALYLSDM
DPTSFEVGFGSSLGIDEDIDSKPLRLRNENLDIIEYEKGICAHAASEIVYDISNQGAKSF
QAYIGVQADSSYDGEYGECGFTVEVDGVEKYHIDDLSGLEAQQFVDVEIPEGAQTLTLKT
DNGTNGSTICDHSLWCDAKILCDREIQTTLDSAAVTAQQSVLPIGKTTQLTPVGYSIGGA
VIPSDTLSAAYASSDDGIATVSPEGVVTGVSDGVVTITCTLTQGDIVREATVDLIIGKGD
PASSWQIASPDGSITALFALHDGKISYASFKDNNLVVDNSYVGLTTSLGDFSELSFVDRV
DEEINDPYELIGAKVTQVENHANQTTLNFTKEGVEGVTFSVIVRAYDDGIAFKYQITAED
ESTPITISSENTSLQIPQGSTAYVMPYINHNEQVEEEKQLSELNERYCMPLLYKTPTGEY
ALISEAGLHGEYCGADIIGNPNGRLDIVFSNEQRSAINTTAPFATPWRFAVIGTSADIAE
NTMAENLSPANVLEDTSWIQPGVTAWTWLNRESTSDPDVYKKYIDFAAEMGWEYLLLDEG
WQPRGSSQGHSEFAYYGYYDWTEDLISYANEKGIGLIAWQNHNDLKKGDEAERRIKELAD
MGFKGIKPDFFNSQSQDYIQFYIRLMQLTAENHMFINIHGANKTTGERRTYPNALSREGI
FGAEQDLFRPADVSAYHNCMLPFMRCAVGPADYTPMFSHRTNTRKQMTIAQMGALTVTYE
SGIQCFADRPEAYLNSPACDFFKNFPTQWDETKLLAGEPGEYVNTARRSGENWYLGLICN
EQRTAEFKLDFLGEGDYYAFIYEDGETVDDITARVETVNKDTVLTIPMAEHGGAMIKILR
NMPSQAESITLSESEVTMEPNETANLTVTFTPEDVEYDTVTWTSSNPEVATVLNGKITAL
KPGLTTITAATGINGNVTATCDVTVRRPEFSLTGDWSIIRSDPENWKLNGENSITITTKP
GEYYSNTANAENVFLTPVEGDFTATTKLTFVPGADYQTAGIIVYQDDKNLYGAYKRYHSH
FNGNIFADFALNNGSASEHTKADPNKDAAVYLKVVKEGTTFTAYYSYDNSEWVQIADPLN
VSGLTGDLKVGLYAVDGNGKSGSLPATFEDFTINGEAVPFANRYIEATGITLDQTELTMK
QYETATLTATVTPEGAQDPITWVSSDPDIATVDENGKITAYEAGTVTITAMTGFNNEVTA
SCEVTIEPADLVLTDDWSIIRNDPEYWTINDEHSITITTQPGEFYTGTDNAQNVILTPAE
GDFTVTTKLDFIPNGDYQTAGIIVYQDTDNIYGAYKRYHSGFGGNIFTDFVMNNGSFSEH
TTPDANKDATVYLKVVKEGTTFSSFYSYDNETWTPIADPVEMSGLSGDLKIGLYAVDGNR
KTGSLPATFEDFTVNGEVIAFAEAPEKEESTSFTVNYGTNAELTLNGEEQTIANLLGKYA
GDFEEGTELNLSFKPSMDGREFSEVLLNGEPQEITDTAEFTYDTVKGADAESLDFSFTVV
SKGVLNTLIDYAETLDEEVAGAVPSVQEKFEKALAEAKTVSETVAVTQQQINDAWGELLD
AIHYLDFQAGDKTELKIQLDIAAQIIEASEGLDPSSIEELKAVMEEAQKVYDNRDAMEPE
ITEAVENLKEAIDNVRHQADKSTLTTVIAKAESIDLSEYIEEGQDTFTAALESAKEIYDD
PSATQEEVDAAADALNAAITALRKIANKDHLKELMETAQSIDASDYTAASYQNLANVMAE
AQKVLDDNTLAEDDPAAGTTIRNMETRLSAAINSLVENSGNSGSGSGSGSSSSSGSTASS
TSTVTTPDFTLDDTTNSCSFRVGERFVFNVRTTSATAPTVTANNANVTVQYLTKTADGYS
FQLNGAAMGESTITVTLNGASSTFTANVQEGSVRSDTTGALSIKKGQAYTFKMTVIDGSS
ATPLFVSGTDGVFKTQFVRQDGNDYYFRIWATGKVGDSSGIYTTMPNQNAVRHCVATIVE

Enzyme Prediction     

No EC number prediction in MGYG000002135_01403.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	303	898	4.8e-168	0.9793977812995246
CBM51	56	200	9.9e-28	0.9850746268656716

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	1.01e-76	555	806	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	1.34e-45	309	549	1	234	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	1.18e-31	809	898	1	96	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.
pfam08305	NPCBM	6.85e-28	56	200	4	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
pfam17851	GH43_C2	4.95e-21	1322	1455	59	203	Beta xylosidase C-terminal Concanavalin A-like domain. This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUO18240.1	1.43e-142	296	897	21	613
AEE97714.1	1.06e-125	305	903	12	595
ATB36826.1	3.30e-118	294	903	14	595
ADO69318.1	1.91e-116	294	903	15	597
AEC02290.1	2.71e-96	309	901	51	627

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	2.49e-70	309	898	6	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	2.76e-69	309	898	20	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5XFM_A	3.82e-66	307	850	19	595	Crystalstructure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_B Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_C Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_D Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron]
5HQ4_A	4.45e-56	307	897	3	657	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	4.45e-56	307	897	3	657	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
D7CFN7	1.97e-83	303	893	33	615	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
Q8A6L0	2.61e-70	305	898	23	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	5.09e-40	306	898	24	723	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
P33747	2.60e-10	1163	1266	20	117	Uncharacterized protein CA_P0160 OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_P0160 PE=3 SV=2
E8MGH9	6.10e-09	1631	1814	1667	1860	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001797	0.996197	0.000444	0.000793	0.000378	0.000362

TMHMM  Annotations     

start	end
7	29