CAZyme Information: MGYG000002135_01837

Basic Information

• Species: Anaeromassilibacillus sp002159845
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Anaeromassilibacillus; Anaeromassilibacillus sp002159845
• CAZyme ID: MGYG000002135_01837
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2876		317797.32	4.0561

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002135	2740278	MAG	United States	North America

• Gene Location: Start: 44361; End: 52991 Strand: -

Full Sequence      

MKREGMLFKRIMGGVLSLTMTLSMVTSTMGTAFAAEKSEEDGYQIYPQVHYADYGTDTLS
LLDGVNVVLEDDLDPYTVSKAETVLEEHGIDATFSEVASEDQVNLFVGVHNDEGEDDAYL
DGKYDASNSVDQIDGYVLSVNSADQTIAITGATSDAAFYGLVTLGQILDQADGEVRDLLI
EDYASIEFRGFIEGFYGTWSHENRKSIMEYCGQFKMNSYLYGPKNDPYHNGQWRDPYPDD
KLAEIKELVELGRETKVQFVWAAHPGGSIDLGSEEDLQALLDKFDQLYSIGVRQFGLFFD
DASTDFTHLDTFAAGIQDYIESKGDVRPLIFCPQVYRKDDGTTGTQNHLKMLDEALPEDV
QIMWTGDWVVSQVDQGMIDWFCEYANRPVYVWWNYPVNDLGRAGYAHMGPSNGLYPDVEN
ISGLVSNPMNQAQISKVSLFSVADYTWNTHDYDSDASWQASFDWVIPDDPEAAEALRIFS
QNSTYGWNPFNAPESAYILEDMEAFEQAYANGEDCTESGEILVDRFQELADAVETLKAYE
GTNGISEELSPWLDKMGNIAVAARDTVQGLMDLDLVPLDNPDAIAMAQQALTDLRAQYQS
ATGTNSKVAASKEIMPFIENIQYLLECRIQLAQGEEPVPRGFGSFAINYAPMLDGSLSTA
TGTDKFSPAAKGAYFGVNLGQMTDITDVVITMDGTHYFKKGLLEWSTDGKTWNEIGEYDT
NIVSVEGLEIQAQYLRYTATDEFVDEFTGSPNRWINIKEFAINKLSEQPDTSKNLALHKP
VTVSTEKPDTTPAAAMTDGDMSTRWGASTGDYNHVEYAIVDLGEVHDIARFTSYFYGETT
RAYLYKIYTSIDGESYELCVDRSNNTTRGKVEDEAPEGTQARYVKIEVSQSNPPQYNASL
YEFEVYAPEDKTVNLALNCNASASADNYGNTPEKAFDGRVAKDSRWAHENGGVGDWLQAD
LGKVCKIDSVKIFWEGINNYQVGYKVQLSNDASEWTDVATVPIAEHEETKELVFDQQQEA
RYVRVLCTEHTGYPSVSIYELEVYGPDPDEEPVDEDVNLALGRFATASNDNYGNTPDKAV
DGKVGKSNRWAVEGGGVGDWLQVDLRQVCKINSVKIFWEGFGSIKIGYKVQLSNDGKQWT
DAATVPIAEHEETKTITFEEQQEARYVRVLCTEHSGYPSISIYELEVYGPKPEPLPGDLD
LDPANYALGKTVEVSSEESNKGNVATYITDGNLETRWAANGGSFPQTVTIDLGREVEISQ
IDTLFYGTEERAYQYTISTSTDGDTFTPCIDRSENADSQWVTDVVPEGTTARYVQYTVTG
LTGVSSGNASIYEVRIWAKDVSNINKAENRFAEASQTTPESSADYALDGDLSTYWASGNI
ENTASLEVTLPAAVPFNRLKLQWNNTDARVDFHIELSDNGTDWKSIYSVGEREMQAEEEF
ILDEQYTSKYVKLVIDAVEGGNGVELCEFGLYQDLDFVGKIDQAKNLLSSIELTDEDTKL
EIPEMPEGVEVTYGGTDYEQVIDANLNILKPLVDTDVMVEYKLVSATNPDETILYEVPMT
VPGQYDAALSQNAKPKVLPELQQWFGHTGDFEITENSRILLDPALDESYKDMADRFAEDY
LDITGNPIQVEIGQNPSAGDFYFKPSDIELEKETYDMEIGDAVTIRMSTETAAFWSTRSI
LQILKQTGTTIPKGLVRDYPKYKVRGFMFDVGRKAIPMEYLEMWVKQMSWYKMNDLNLHL
SDNSFDNTYAGFRLESDVPGLSSTDLHYKKDEFRNFLLDSKAQGVNIVPEFDTPGHSLAF
VWARPDLGRDGPNSSYLDVSNPETIEFIKSVWAEYMQEEDPVFLEDTVVNIGTDEYKGSN
QEGKEAFRKYQDDLLRFIRDEMNHTPRLWGSQTENDGVTPITAENVQMYMWYVGYADAQE
MYDKGYQMININDGDVYIVPGAGYYWDYLSKSHILNTYDPTKIYGGKLVVPAGDPQMLGG
SYALWNDRTGKGEISEGVPRDNGTTDVELFDRMFDILPTFGEKLWSSCKDYDVAAIDALT
EEVLYAPNTNPTYEVDSNTQTVLRYDFEEGVETRDSSANANNLLGEGVNVSYTDGRDGKA
LTLAGGESYVETPVSDARINTTLDFWVKRDADSDGSEQILFESSIGAIKAVQKETGKFGF
SRDFRDYSFDYTLPKGEWVHITMVMEFTKTTLYVNGEKVHTLENTATGGNQWASLITPLN
RIGSTTAAFKGQIDDVAFYTYASSADQLNALPVDLLDAETGVTVHGDSGVLPADSELFVE
DLSSGAAVDALKNSLAGTQERFKAYAVDVQSGGATVTPHGVATISAPVPAQLNPDTLRVV
EVSNDGVIRNVDCTVKNGVVVWQSDHFNADSAFAVVGTVASSSVVITYNSKDVTLKVNGE
PQKIADLLGKYVIEDMTDDMQVELTFEPRREGEQFRAVTVNGKTENITGSSYTYTVTAKD
VGTESDFLFDVTNKTILEQLYAYAKTYVDDGTVDKLIPSVKEAFMKAYDAAGKVIEDPAA
TQAEIDQAWSDLLDVIHYLDFQPGDKSALQDLYDILKNLSESDYTSTSWAPFAEALQQAA
DVLADNEALENDITAAYDVLLNAAQALVKVADRTSLDAALAKAETVAKDIEAGKYLPDGQ
EEFQTAYAAAKALDQNATQSAIDEAAQALVKAMLNLRKIPSREELKAYIAEMERVDLDAY
TASSAATFRSALAAAKAVAADEEADGQMLATAFYQLQDAEQGLEKAETPAPNPGNGSKGS
TSQNSSNAYGAAGVVSAGQSVAAAGAYVRSDTTVNFTMKRGQAYCFKMTVCNGDNTMVPS
FTVGDGSVFKTQFVARIGNDFYYRIWAVGESGQSSGIYTTLPNGAPQKHCVVTIGA

Enzyme Prediction     

No EC number prediction in MGYG000002135_01837.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	189	480	1.2e-96	0.9932203389830508
GH20	1696	2027	1.2e-57	0.9762611275964391
CBM32	1066	1186	7.1e-23	0.9274193548387096
CBM32	922	1042	1.7e-22	0.9274193548387096
CBM32	1215	1334	1.7e-20	0.9193548387096774

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	9.32e-127	189	479	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
cd06564	GH20_DspB_LnbB-like	1.59e-111	1703	2025	1	324	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
cd02742	GH20_hexosaminidase	2.97e-27	1705	2026	2	303	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	5.85e-27	1702	2026	1	344	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
COG3525	Chb	6.97e-24	1631	1987	183	589	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATD55826.1	1.12e-251	1205	2751	41	1678
SLK21750.1	1.12e-251	1205	2751	41	1678
QBJ76126.1	1.12e-251	1205	2751	41	1678
ATD56500.1	1.03e-249	1205	2751	41	1678
AYE33718.1	5.71e-239	1205	2743	41	1670

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	1.13e-108	30	560	18	557	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6JQF_A	1.89e-93	1508	2233	26	726	Crystallizationanalysis of a beta-N-acetylhexosaminidase (Am2136) from Akkermansia muciniphila [Akkermansia muciniphila ATCC BAA-835]
2J4G_A	2.81e-80	131	553	75	498	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2JIW_A	2.88e-80	131	553	76	499	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with 2- Acetylamino-2-deoxy-1-epivalienamine [Bacteroides thetaiotaomicron VPI-5482],2JIW_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with 2- Acetylamino-2-deoxy-1-epivalienamine [Bacteroides thetaiotaomicron VPI-5482]
2J47_A	2.88e-80	131	553	76	499	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with a imidazole-pugnac hybrid inhibitor [Bacteroides thetaiotaomicron VPI-5482],2W4X_A BtGH84 in complex with STZ [Bacteroides thetaiotaomicron VPI-5482],2W66_A BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W66_B BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W67_A BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2W67_B BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2WCA_A BtGH84 in complex with n-butyl pugnac [Bacteroides thetaiotaomicron VPI-5482],2XJ7_A BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XJ7_B BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XM1_A BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM1_B BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM2_A BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],2XM2_B BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],4UR9_A Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],4UR9_B Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],5FKY_A Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FKY_B Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FL0_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL0_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B2UPR7	2.42e-97	1484	2233	22	748	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1
P26831	1.37e-85	9	1145	6	1147	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	2.49e-79	131	553	97	520	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	4.31e-68	57	777	58	781	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	7.68e-68	57	777	58	781	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001263	0.997355	0.000286	0.000451	0.000338	0.000278

TMHMM  Annotations     

start	end
13	35