CAZyme Information: MGYG000002148_01715

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eisenbergiella
• CAZyme ID: MGYG000002148_01715
• CAZy Family: GH38
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1045	MGYG000002148_12|CGC2	120971.43	4.926

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002148	4597814	MAG	United States	North America

• Gene Location: Start: 34067; End: 37204 Strand: -

Full Sequence      

MFFTDRKLEGRTRELEEYRYRDVTAIEKFLAAEDTQGVVNPEVPDCFEGWNVMKIGENWA
GRDKYLWLHTEVEIPAEWKGRRAVGVFDFGFTGAGNNSGFESLLYIDGEPYQGVDVNHKE
VFFKESMYGRKLSLTFRLWSGLEGGGLPKEMEHKLKRAKFGWLDEKTDDLYYMSDMVLKT
VAQLSENDPVKHDLLANLDRAMHCIDWSYPGSETFYESVAQADDMLNEKIDAMGKRSDIN
VYAVGHTHIDIAWLWRLKNTREKCSRSFSTVMRMMEMFPEYIFLQTQPQLYEYVKEDFPE
LYEQIRERIAQGRWEADGAMWVEADCNLTSGESLTRQILIGSKFIKEEFDKEVEYLWLPD
VFGYSWALPQILKKSGIDMFMTTKISWNQYNRMPHDTFYWKGIDGSEVLTHFITTPEPDS
PADSWFYTYNGNLLPRTVKGVWDSYSEKDMNKDLLISYGFGDGGGGVNRDMLEQRRRLDR
IPGLPNVKTCTAGEYFRKLKENVKNTDQFVNVWDGELYLEYHRGTYTSHAHNKRMNRKME
LLYREAEWLTVMRALAAGGDLSLAQQEELTKGWKHILTDQFHDIIPGSSIFEVYEDSKKD
YEWIAGIGEAVEQTFFEDVQKPEKGRYTVINSNGFMMDGPVLLEGAKGSAYLKDGTKLDS
QETEKGLLVHAKAVPSMGRLEIWVKEEENGAAKPFTVSGDGHVLETPFYVMSLNDYGQIT
RLYDKEFEREVLPKGQRANVLQVFEDKPMGNDAWDIDIFYQQKMREITDLCAYEVVEEGP
VRLKIRMKWHYMNSAVSQDMILYAADRRIDFETEVDYHERHQLLKAAFPVDIRTTYGTFD
VQYGNVRRPNHWNTSWDQAKFETVAHRFADLSEYGYGVSLLNDCKYGHDVKDNVLRITLI
KTATSPDHSQDQGMHYFTYSLLPHGGSFVEGRTVQEACALNQPMHAVEGRLNLPFESFLS
FDRESVELDAVKKTEDGKYIAVRFHDFTGGTNRITVTPGFTWEAYCESDLRERPLTEWKK
EGPVTVTVKPYEIITLLFAPLRDGE

Enzyme Prediction     

EC	3.2.1.24

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH38	240	507	6.1e-84	0.9479553903345725

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0383	AMS1	0.0	7	1039	3	942	Alpha-mannosidase [Carbohydrate transport and metabolism].
cd10789	GH38N_AMII_ER_cytosolic	1.50e-124	241	501	2	251	N-terminal catalytic domain of endoplasmic reticulum(ER)/cytosolic class II alpha-mannosidases; glycoside hydrolase family 38 (GH38). The subfamily is represented by Saccharomyces cerevisiae vacuolar alpha-mannosidase Ams1, rat ER/cytosolic alpha-mannosidase Man2C1, and similar proteins. Members in this family share high sequence similarity. None of them have any classical signal sequence or membrane spanning domains, which are typical of sorting or targeting signals. Ams1 functions as a second resident vacuolar hydrolase in S. cerevisiae. It aids in recycling macromolecular components of the cell through hydrolysis of terminal, non-reducing alpha-d-mannose residues. Ams1 utilizes both the cytoplasm to vacuole targeting (Cvt, nutrient-rich conditions) and autophagic (starvation conditions) pathways for biosynthetic delivery to the vacuole. Man2C1is involved in oligosaccharide catabolism in both the ER and cytosol. It can catalyze the cobalt-dependent cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl-enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst.
pfam01074	Glyco_hydro_38	1.02e-90	240	501	1	257	Glycosyl hydrolases family 38 N-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism.
cd10813	GH38N_AMII_Man2C1	4.40e-78	241	502	2	252	N-terminal catalytic domain of mammalian cytosolic alpha-mannosidase Man2C1 and similar proteins; glycoside hydrolase family 38 (GH38). The subfamily corresponds to cytosolic alpha-mannosidase Man2C1 (also known as ER-mannosidase II or neutral/cytosolic mannosidase), mainly found in various vertebrates, and similar proteins. Man2C1 plays an essential role in the catabolism of cytosolic free oligomannosides derived from dolichol intermediates and the degradation of newly synthesized glycoproteins in ER or cytosol. It can catalyze the cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Man2C1 is a cobalt-dependent enzyme belonging to alpha-mannosidase class II. It has a neutral pH optimum and is strongly inhitibed by furanose analogs swainsonine (SW) and 1,4-dideoxy-1,4-imino-D-mannitol (DIM), moderately by deoxymannojirimycin (DMM), but not by kifunensine (KIF). DMM and KIF, both pyranose analogs, are normally known to inhibit class I alpha-mannosidase.
pfam07748	Glyco_hydro_38C	4.10e-71	704	909	1	204	Glycosyl hydrolases family 38 C-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU77101.1	0.0	1	1039	1	1039
QQQ94677.1	0.0	1	1039	1	1039
ASU29899.1	0.0	1	1039	1	1039
QJU17275.1	0.0	1	1039	1	1039
QIB56865.1	0.0	1	1039	1	1039

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6LZ1_A	3.59e-132	56	1035	98	1072	Structureof S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_B Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_C Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_D Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-]
7DD9_A	4.88e-129	56	1035	98	1072	ChainA, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_C Chain C, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_E Chain E, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_G Chain G, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct]
5JM0_A	8.63e-117	155	976	213	1009	Structureof the S. cerevisiae alpha-mannosidase 1 [Saccharomyces cerevisiae S288C]
1HWW_A	3.27e-06	239	622	51	482	GOLGIALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE [Drosophila melanogaster],1HXK_A Golgi Alpha-Mannosidase Ii In Complex With Deoxymannojirimicin [Drosophila melanogaster]
6RPC_A	3.28e-06	239	622	69	500	ChainA, Alpha-mannosidase 2 [Drosophila melanogaster],6RQZ_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRH_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRJ_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRN_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRU_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRW_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRX_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRY_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RS0_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q54K67	4.25e-157	54	1038	70	1084	Alpha-mannosidase G OS=Dictyostelium discoideum OX=44689 GN=manG PE=1 SV=1
Q9NTJ4	2.33e-146	54	1037	68	1032	Alpha-mannosidase 2C1 OS=Homo sapiens OX=9606 GN=MAN2C1 PE=1 SV=1
Q91W89	1.53e-139	48	975	61	963	Alpha-mannosidase 2C1 OS=Mus musculus OX=10090 GN=Man2c1 PE=1 SV=1
P21139	1.68e-137	48	975	61	964	Alpha-mannosidase 2C1 OS=Rattus norvegicus OX=10116 GN=Man2c1 PE=1 SV=1
Q9UT61	6.05e-132	56	1035	98	1072	Alpha-mannosidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ams1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000016	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002148_01715.