CAZyme Information: MGYG000002163_00359

Basic Information

• Species: Methanobrevibacter_A smithii
• Lineage: Archaea; Methanobacteriota; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter_A; Methanobrevibacter_A smithii
• CAZyme ID: MGYG000002163_00359
• CAZy Family: GT66
• CAZyme Description: Dolichyl-phosphooligosaccharide-protein glycotransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
922	MGYG000002163_10|CGC2	100592.59	5.1842

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002163	1727775	Isolate	not provided	North America

• Gene Location: Start: 331289; End: 334057 Strand: -

Full Sequence      

MNKKTILTISKSAIIILILLAVVFALRAPAADLNILPTGDLKAEYEDASGLPYFSEMDSY
YNLRLTENFVDHGYVGDELVDGSNWDMHRNSPDGDKINYELAIVWVTSFFYNIANQFFGD
YTVQEVAFWTGAIVASLAVVPAFIFARRLTNDLGAITATLIIVLAPNYFAHTFPGFFDTD
MFYYIFSLFFIMFFMESLRSKNLIAKVVFAILSIVSIGLFSQSWTGYIFYVGLMGIFSVV
YLILCYVFNIGDSERELYPSKAAWFVHQKDLMSIVILGVIGFIGLAAFKGVDGVLGIFGS
LTGLLSLQSTSTVVGGFPNVLISVAELQMPSMLGAGMDSFLLANSNGVINGIGGIFVFFA
GLTVLFIFAKRIYDYRNVKQDRNTTKKPPKSQRLASSKKIDDDRKFKISLGSVSKFAKDN
EVTEAKRLNLMFATLFIVWVVITALAVSRGSRFITTIVLPFGLMAGIFVGYASDYVKTKL
DNDKMLMAVILVTGFFASYPLAAVTVPIFGIILFLLIAIAGGITIYGLKSSKSASTKVPV
KKYVAIIAIALALITPTVCGAYQTANQVVPGTSDAMWDSMAWINENTADNTVVASWWDFG
YLFEIAADRQVIFDGGSQSGNSRAFWLGQAMTTDNMDLSAGIFRMLGTSGENATNTLTDY
TGSPGKATDILIDILPKNAQDAKNTLINTYGLTTEQANTIIPLTHPDNPRPVIFVASSDM
LQKAGWWSYFGAWDFDKQNSTNFNYYVPSQQVTVEPGSTGRLALINESGLEYDAVITRGT
GNNTTTGHTEAVYSNNGSLLKINGSEFNPLNVSRIMVIEGNQLVKNESIAGAPSDSNYTL
FLMGENNVYTPIIMHNKLADSMFTRLYLLGGMGQNVFSMVHMENGVSLWQVNYNNTAAGG
TSTPAASGATDGNIGTTSPDRA

Enzyme Prediction     

No EC number prediction in MGYG000002163_00359.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	22	798	1.8e-144	0.9812409812409812

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1287	Stt3	2.12e-56	3	674	6	605	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].
pfam02516	STT3	1.04e-54	18	617	1	478	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATZ58987.1	0.0	1	922	1	922
ABQ86921.1	0.0	1	922	1	922
ALT69910.1	0.0	1	900	1	898
AMD16758.1	0.0	1	899	1	896
AGN16102.1	1.87e-306	1	913	1	902

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2EMT4	6.82e-73	10	879	36	889	Dolichyl-phosphooligosaccharide-protein glycotransferase OS=Methanococcus voltae OX=2188 GN=aglB PE=1 SV=1
Q58920	2.57e-52	11	893	26	927	Dolichyl-phosphooligosaccharide-protein glycotransferase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=aglB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.273389	0.723207	0.002297	0.000389	0.000319	0.000378

TMHMM  Annotations     

start	end
12	31
127	146
153	171
181	200
207	224
228	250
271	288
347	369
428	447
451	473
485	504
508	530
543	565