CAZyme Information: MGYG000002178_01072

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; CAG-617
• CAZyme ID: MGYG000002178_01072
• CAZy Family: GH33
• CAZyme Description: Sialidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
400	MGYG000002178_77|CGC1	43961.32	7.3196

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002178	2197502	MAG	Spain	Europe

• Gene Location: Start: 2249; End: 3451 Strand: -

Full Sequence      

MKKKHLLAWLLALLPWTGGAQTVLFHTGDSTGYPYRIPAIATARDGQLVALSDRRPCGGD
IGYGRVDILMRTSHDDGETWTPAVTVLEGTGSGATTGYGDACLVADRERNELLLVCAAGD
VPYWSSRPSHPLRMVYTRARYDKRGKTWRWDAPTDITGQIYFDVFGGRINGLFMGSGRIC
QSARVKTGDYYRLYAALATTSGNFVIYSDNFGRTWQALGGAEASPAPRGDEPKCEELPDG
SVLLSSRKAGGRYFNIFRYTDVGRAEGSWAEAVDSKQARGGIANESGACNGEILIVPARD
VAGRKVELALQSLPAGPARQNVTIYYKELVTAADYDTPAHFAEDWDGAYRVSHTGSAYST
MTLQADGRIGFFYEEEPGWYQMVYRALTLEEITKGRYRSR

Enzyme Prediction     

No EC number prediction in MGYG000002178_01072.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH33	22	378	1.8e-51	0.9415204678362573

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd15482	Sialidase_non-viral	1.59e-64	22	388	1	339	Non-viral sialidases. Sialidases or neuraminidases function to bind and hydrolyze terminal sialic acid residues from various glycoconjugates, they play vital roles in pathogenesis, bacterial nutrition and cellular interactions. They have a six-bladed, beta-propeller fold with the non-viral sialidases containing 2-5 Asp-box motifs (most commonly Ser/Thr-X-Asp-[X]-Gly-X-Thr- Trp/Phe). This CD includes eubacterial and eukaryotic sialidases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12979.1	2.61e-182	3	397	2	397
EFC70822.1	2.81e-153	20	400	19	405
QYR10380.1	3.16e-114	20	398	22	396
ALO48969.1	2.07e-111	17	397	22	399
QFQ13500.1	2.03e-88	29	399	632	1009

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1EUR_A	1.64e-15	32	378	21	348	Sialidase[Micromonospora viridifaciens],1EUS_A Sialidase Complexed With 2-Deoxy-2,3-Dehydro-N- Acetylneuraminic Acid [Micromonospora viridifaciens]
1EUT_A	3.50e-15	32	378	21	348	Sialidase,Large 68kd Form, Complexed With Galactose [Micromonospora viridifaciens],1EUU_A Sialidase Or Neuraminidase, Large 68kd Form [Micromonospora viridifaciens]
1W8N_A	8.25e-15	32	378	17	344	Contributionof the Active Site Aspartic Acid to Catalysis in the Bacterial Neuraminidase from Micromonospora viridifaciens. [Micromonospora viridifaciens],1W8O_A Contribution of the Active Site Aspartic Acid to Catalysis in the Bacterial Neuraminidase from Micromonospora viridifaciens [Micromonospora viridifaciens]
1WCQ_A	1.10e-14	32	378	17	344	Mutagenesisof the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine. [Micromonospora viridifaciens],1WCQ_B Mutagenesis of the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine. [Micromonospora viridifaciens],1WCQ_C Mutagenesis of the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine. [Micromonospora viridifaciens]
2BZD_A	1.95e-14	32	378	17	344	Galactoserecognition by the carbohydrate-binding module of a bacterial sialidase. [Micromonospora viridifaciens],2BZD_B Galactose recognition by the carbohydrate-binding module of a bacterial sialidase. [Micromonospora viridifaciens],2BZD_C Galactose recognition by the carbohydrate-binding module of a bacterial sialidase. [Micromonospora viridifaciens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q02834	2.01e-14	32	378	63	390	Sialidase OS=Micromonospora viridifaciens OX=1881 GN=nedA PE=1 SV=1
P15698	2.05e-14	21	388	38	391	Sialidase OS=Paeniclostridium sordellii OX=1505 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000617	0.998521	0.000243	0.000214	0.000196	0.000184

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002178_01072.