CAZyme Information: MGYG000002216_00351

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae
• CAZyme ID: MGYG000002216_00351
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1866		208108.74	4.4364

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002216	3003083	MAG	Spain	Europe

• Gene Location: Start: 14320; End: 19920 Strand: +

Full Sequence      

MKKRIMSILLSLTMIAGSCLPSMGTVFAAGEEPQVQTVAAENGERRGNLNYFSNQGKLNE
DAFVQLPMGAVEAKDWLKQQLYLQKNGLTGAIHDEYPLYGPDNGWRGGSGDVWEKGPYYL
RGLVSLAWVLDDEELKDKAMEWINFILDNQRDNGFMGPLSDGDGSSNSWDWWPRMVILQV
IRDYYEATELEGEPDARVMPFFEKYFRYQAERLHQKPLTDWAASRGGDNIEVLLWYYNRV
YSALNPTETDWIIDLALLLAGQTKSQNSGLDWNTVFNDTTVREHVVNTTQAMKTPAVLSQ
LPDHAQDADSLKQGIFNMGIDHGRVDNLANADEAARENYPYRGAELCSVVESLLSNEMSI
RITGESWLGDQMEQTAYNNLPAGYAPDYTGHNYFQAQNQVLATHGYHEFENDHGDSCSYG
ALTGFECCFPNMHMGWPKFVQSMWMATKDNGLAVVAYGPNTVTAKVADGKTAVFDEVTDY
PFEDTIALHYSGDTAQFPLKLRLPAWCEEPVVTVNGDRADLSVQEKGFAVVNRQWEAGDQ
VVVTFPMEVRASTWYNNAQAVEYGPLIFALKVEEDWRIATDDEAREIKYEPVGEFDRKEV
YPASDWNYALVLDEEDLQSSFEIIKQEDVALQPFTLSNAPITMKVKAQQVPQWKLKGNVV
PEPPYSPIAEDESLQTEVELVPYGCTRIRITQMPTVGTPSEDGITERTMEDAKVYEEDGV
KVTEFDNVVVPYAEDYILKVSYTGSGPMALNINEKYQETVEFGGSGTYTLENLNDIVPGT
NQYFDFGYGKYNNLRFYGDAVEITKIEILPVELFTEPVIKSAVLSEDGTSVTLNTNIDRG
AGFYTVRYGNESRNYTMTAENFFEKGATITGLTPGEEYYFQISMLVNGVEKTSNEIKAEK
PGEGEKIPSFKDDFSNAEASKEKWTVYDPNDAVQFTDGSLQVGTSPNVKIMAGSGDQIWT
DYAVETTLKGPENPQRDFGVMFRCTNVTGEGADSYQGYYVGIDAIGNGLNVGYANGSWND
IAKVPAFTYEPNKDYKLKILVCGNQFKVYVDGELAYELTDDKFAYGAVGLRSWNQPFEAN
SFQVRELTTEEKALFGGEQEPQEPQEPEAVAPEFIDDFEDAEASAQVWRLCGDQSKMHFI
GGKLSMDSSNNVKAVTGEDGWRDYVAEVSITLSGDSTQNAGLMYRVSQVEDQGSDGYHGY
FYGIGNNNDGTGYFILGYADGGWHQTRRSNLPAFENGKAYRLKVVVSGDRVAIFLDDELL
TRFVDNRYSYGKIGLRSYEKPFSADDVTVRPFTEDDEAVFDGMSRYVDETFGSYKTIQLK
FPKFSSTGKYKIEYGTESGKYTNEVYNLSHNVSWNGQSVSSDKLGLSLPENNKDYYLRLI
GLNGNQETIISDEVKVHTGDKWDLTEEMEKLSDCLEEATQTDAQGCTEESVYRLEWAIQN
AEKVSQEADSNLIDVRLAKNCLKVGMNELEKEQGEVEEADKTGLQFVYDLYKDKKESNYT
PESWKAFAEALARAEELLADEKVSQQEVDEAAKALQDAAEALVSVGGGLTEEDLKEAVEA
AEAAKEAAEKAQELAQAAKEEADRLAGEAAEAQKKAEEAWEKAQDMADASEEEKQEAIAQ
AEEATRQAEAAQKKAEEAQEALERAEAARDAALKAVESYQAEAEAIRSRLQAAKDEIAKA
EEEARKAKEEAERLKKEAEESARLAEETLKKLQENSQKQETEDTVKKGDIYTVGKLKYQV
TDVKKKTVTVTGAKKKGQKTVTIPATVKIHGESYKVTAVAKKAFYKNSSLRKVTIGKNVT
EIGKNAFGNTRNLKSVVIKTKNLKKVGKGAFKGVHKKCVIRVPSKKAKAYKKLIKKSGIA
KTVKVK

Enzyme Prediction     

No EC number prediction in MGYG000002216_00351.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	344	568	5.4e-31	0.42366412213740456
CBM66	933	1075	7e-21	0.8709677419354839
CBM66	1147	1279	4.2e-17	0.8

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	5.79e-44	100	569	50	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	4.72e-27	344	570	291	502	Uncharacterized conserved protein, DUF1680 family [Function unknown].
PTZ00121	PTZ00121	2.80e-15	1429	1804	1197	1541	MAEBL; Provisional
PTZ00121	PTZ00121	1.34e-12	1386	1805	1388	1806	MAEBL; Provisional
sd00036	LRR_3	1.56e-12	1759	1845	63	139	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALL07583.1	7.97e-147	44	783	21	745
QJE95537.1	3.79e-145	54	700	31	652
ATP55412.1	2.39e-142	51	703	28	658
AMP98056.1	7.41e-140	51	703	28	658
QNK64903.1	1.83e-139	38	715	12	670

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	2.30e-15	344	571	332	562	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.07e-14	344	571	332	562	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000553	0.632393	0.365913	0.000584	0.000315	0.000214

TMHMM  Annotations     

start	end
7	29